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|Application / Reactivity||Human||Mouse (Murine)||Rat (Rattus)|
|Immunofluorescence (fixed cells) (IF/ICC)||3 Antibodies||1 Antibodies|
|Western Blotting (WB)||160 Antibodies||78 Antibodies||49 Antibodies|
|Proximity Ligation Assay (PLA)||3 Antibodies|
|Immunoprecipitation (IP)||30 Antibodies||19 Antibodies||17 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||46 Antibodies||31 Antibodies||21 Antibodies|
|Immunohistochemistry (IHC)||69 Antibodies||39 Antibodies||22 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||9 Antibodies||6 Antibodies||5 Antibodies|
|Antigen||X-Linked Inhibitor of Apoptosis (XIAP) Antibodies|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This XIAP antibody is un-conjugated Alternatives|
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Product Details anti-XIAP AntibodyTarget Details XIAP Application Details Handling Images
|Specificity||This antibody detects endogenous levels of XIAP protein. (region surrounding Gly81)|
Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.
|Purification||The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen|
|Purity||> 95 % pure by SDS-PAGE|
Target Details XIAPProduct Details anti-XIAP Antibody Application Details Handling Images back to top
|Alternative Name||IAP3 / BIRC4 (XIAP Antibody Abstract)|
|Background||The human X chromosome linked Inhibitor of Apoptosis Protein (XIAP) is a major member of the inhibitor of apoptosis (IAP) family. XIAP functions by direct binding and inhibition of several caspases, including Caspase 3, Caspase 7 and Caspase 9. During apoptosis XIAP is specifically cleaved by Caspase 3 and partially loses its function. In addition to blocking the apoptosis signaling pathway, XIAP can also bind several other proteins in the bone morphogenetic protein (BMP) signaling pathway and act as a coupler between the membrane BMP receptor and the TAB1 TAK1 complex by binding to these proteins.Synonyms: API3, Baculoviral IAP repeat-containing protein 4, E3 ubiquitin-protein ligase XIAP, HILP, Inhibitor of apoptosis protein 3, X-linked IAP, X-linked inhibitor of apoptosis protein, XIAP|
|Molecular Weight||approx. 57 kDa|
|Research Area||Cancer, Apoptosis/Necrosis|
|Pathways||Apoptosis, Caspase Cascade in Apoptosis, Transition Metal Ion Homeostasis|
Application DetailsProduct Details anti-XIAP Antibody Target Details XIAP Handling Images back to top
ELISA: 1: 20000approx. 1: 40000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-XIAP Antibody Target Details XIAP Application Details Images back to top
|Buffer||Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||DO NOT FREEZE!|
|Storage Comment||Store the antibody undiluted at 2-8 °C.|
ImagesProduct Details anti-XIAP Antibody Target Details XIAP Application Details Handling back to top