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|Antigen||Occludin (OCLN) Antibodies|
|Reactivity||Dog (Canine), Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This Occludin antibody is un-conjugated Alternatives|
Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), ELISA, Western Blotting (WB)
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Product Details anti-Occludin AntibodyTarget Details Occludin Application Details Handling Images
|Specificity||Recognizes human occludin at ~65kD. Reactivity of this antibody with the occludin protein has been confirmed by Western Blot using total cell lysates derived from MDCK cells, human T84 cells, human Caco-2 and rat fibroblasts transfected with a plasmid encoding human occludin. Species cross-reactivity has been confirmed in Western blot only.|
A GST fusion protein consisting of the C-terminal 150 amino acids of human occludin fused to GST.
Type of Immunogen: Fusion protein
Target Details OccludinProduct Details anti-Occludin Antibody Application Details Handling Images back to top
|Alternative Name||OCLN / Occludin (OCLN Antibody Abstract)|
Name/Gene ID: OCLN
Synonyms: OCLN, Occludin, BLCPMG
|Pathways||Cell-Cell Junction Organization, Hepatitis C|
Application DetailsProduct Details anti-Occludin Antibody Target Details Occludin Handling Images back to top
Approved: ELISA (0.1 - 1 μg/mL), IF, IHC-P (2.5 μg/mL), IP, WB (1 - 3 μg/mL)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL.
|Restrictions||For Research Use only|
HandlingProduct Details anti-Occludin Antibody Target Details Occludin Application Details Images back to top
|Buffer||PBS, pH 7.4, 0.09 % sodium azide.|
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeat freeze-thaw cycles.|
|Storage||4 °C,-20 °C|
Long term: -20°C
Short term: +4°C. Avoid repeat freeze-thaw cycles.
ImagesProduct Details anti-Occludin Antibody Target Details Occludin Application Details Handling back to top