anti-Mouse (Murine) Occludin antibody for Immunoprecipitation

Recommended Occludin Antibody (supplied by: Log in to see )

Antigen
Occludin (OCLN) Antibodies
  • tpmt
  • OCLN
  • BLCPMG
  • AI503564
  • Ocl
  • ocln
  • oclnb
  • wu:fd23h10
  • wu:fi13c01
  • zgc:113992
  • zgc:56359
  • occludin
  • occludin a
  • OCLN
  • ocln
  • Ocln
  • ocln-b
  • oclna
Epitope
C-Term
33
15
13
7
7
6
5
5
4
4
3
3
2
2
1
1
Reactivity
Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
111
55
49
17
16
4
4
4
3
2
2
2
Host
Rabbit
85
30
3
Clonality
Polyclonal
Conjugate
This Occludin antibody is un-conjugated
6
6
5
3
3
3
1
1
1
1
1
1
1
1
1
1
1
Application
Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), ELISA, Western Blotting (WB)
100
54
28
25
21
13
10
6
3
1
1
1
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Catalog No. ABIN305888
$ 676.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN1175609 ICC IHC IP WB Rabbit IgG AA 17-107 Log in to see Polyclonal

General

Antigen Occludin (OCLN) Antibodies
Epitope C-Term
(33), (15), (13), (7), (7), (6), (5), (5), (4), (4), (3), (3), (2), (2), (1), (1)
Reactivity Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
(111), (55), (49), (17), (16), (4), (4), (4), (3), (2), (2), (2)
Host Rabbit
(85), (30), (3)
Clonality
Polyclonal
Conjugate This Occludin antibody is un-conjugated
(6), (6), (5), (3), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), ELISA, Western Blotting (WB)
(100), (54), (28), (25), (21), (13), (10), (6), (3), (1), (1), (1)
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Product Details anti-Occludin Antibody

Target Details Occludin Application Details Handling Images
Brand IHC-plus™
Specificity Recognizes human occludin at ~65kD. Reactivity of this antibody with the occludin protein has been confirmed by Western Blot using total cell lysates derived from MDCK cells, human T84 cells, human Caco-2 and rat fibroblasts transfected with a plasmid encoding human occludin. Species cross-reactivity has been confirmed in Western blot only.
Purification Immunoaffinity purified
Immunogen A GST fusion protein consisting of the C-terminal 150 amino acids of human occludin fused to GST.

Type of Immunogen: Fusion protein
Isotype IgG

Target Details Occludin

Product Details anti-Occludin Antibody Application Details Handling Images back to top
Antigen
Alternative Name OCLN / Occludin (OCLN Antibody Abstract)
Background Name/Gene ID: OCLN

Synonyms: OCLN, Occludin, BLCPMG
Gene ID 100506658
UniProt Q16625
Pathways Cell-Cell Junction Organization, Hepatitis C

Application Details

Product Details anti-Occludin Antibody Target Details Occludin Handling Images back to top
Application Notes Approved: ELISA (0.1 - 1 μg/mL), IF, IHC-P (2.5 μg/mL), IP, WB (1 - 3 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL.
Restrictions For Research Use only

Handling

Product Details anti-Occludin Antibody Target Details Occludin Application Details Images back to top
Format Liquid
Concentration Lot specific
Buffer PBS, pH 7.4, 0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeat freeze-thaw cycles.
Storage 4 °C,-20 °C
Storage Comment Long term: -20°C
Short term: +4°C. Avoid repeat freeze-thaw cycles.

Images

Product Details anti-Occludin Antibody Target Details Occludin Application Details Handling back to top
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Occludin (OCLN) (C-Term) antibody (ABIN305888) Human Kidney (formalin-fixed, paraffin-embedded) stained with OCLN antibody ABIN30588...