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anti-Human C3 antibody for Enzyme Immunoassay

Recommended C3 Antibody (supplied by: Log in to see )

Antigen
Complement Component 3 (C3) Antibodies
  • C3
  • C3-1
  • sb:cb26
  • si:dkey-76b14.4
  • wu:fi34b03
  • wu:fj86e11
  • asp
  • cpamd1
  • AI255234
  • ASP
  • HSE-MSF
  • Plp
  • AHUS5
  • ARMD9
  • C3a
  • C3b
  • CPAMD1
  • C3d
  • c3
  • c3_D
  • complement component c3a
  • complement component 3
  • complement C3-like
  • complement component C3
  • endogenous retrovirus group K, member 13
  • c3a
  • C3
  • c3
  • LOC100060539
  • c3-1
  • ERVK-13
Reactivity
Human
264
73
61
15
7
6
5
4
3
1
1
1
Host
Mouse
133
110
91
25
14
8
Clonality
Monoclonal
Conjugate
This C3 antibody is un-conjugated
31
29
18
5
5
4
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
193
182
70
65
60
52
45
42
41
39
35
24
11
5
4
3
2
2
2
1
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN181105
$ 577.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN1106781 EIA WB Mouse IgG1 C-Term Log in to see 2898 2
1 ABIN112153 EIA IHC (fro) WB Mouse IgG1 Log in to see H206 1
1 ABIN317012 EIA FACS IHC (fro) WB Mouse IgG2b Log in to see 013III-1-16 3
1 ABIN1106782 EIA WB Mouse IgG1 C-Term Log in to see 755 1
1 ABIN1106783 EIA WB Biotin Mouse IgG1 Log in to see 474 4
1 ABIN1107627 EIA IP Rat IgG1 Log in to see 9 5
1 ABIN181106 EIA FACS IHC (fro) WB Mouse IgG1 Log in to see 10-02A 1
1 ABIN181107 EIA FACS IHC (fro) WB Mouse IgG1 Log in to see 053A-1149-3-1-4 (1003) 2
1 ABIN181129 EIA FACS IHC (fro) Mouse IgG1 Log in to see 053A-514-3-1-4 2
1 ABIN111064 EIA IHC (fro) WB Mouse IgG2b Log in to see H13 3
1 ABIN1106778 EIA WB Mouse IgG1 Log in to see 474 4
1 ABIN951635 EIA FACS WB Chicken Ig Log in to see Polyclonal 1
1 ABIN113306 EIA WB HRP Sheep Log in to see Polyclonal 1
1 ABIN951634 EIA FACS WB HRP Chicken Ig Log in to see Polyclonal 1
1 ABIN784157 EIA IHC (fro) IHC (p) Sheep IgG Log in to see Polyclonal
1 ABIN113307 EIA ID Sheep Log in to see Polyclonal
1 ABIN568570 DB EIA ID IHC (fro) IF IP WB Biotin Goat IgG Log in to see Polyclonal
1 ABIN568571 EIA ID IHC (fro) IF IP FITC Goat IgG Log in to see Polyclonal
1 ABIN568572 DB EIA ID IHC (fro) IF IP WB HRP Goat IgG Log in to see Polyclonal
1 ABIN568574 EIA ID IHC (fro) IF IP TRITC Goat IgG Log in to see Polyclonal

Top referenced anti-C3 antibody for Enzyme Immunoassay

Similar anti-C3 Antibodies

Application / Reactivity Human
Dot Blot (DB) 2 Antibodies
ELISA 124 Antibodies
Enzyme Immunoassay (EIA) 28 Antibodies
Flow Cytometry (FACS) 26 Antibodies
Functional Studies (Func) 1 Antibodies
Immunoassay (IA) 5 Antibodies
Immunocytochemistry (ICC) 7 Antibodies
Immunodiffusion (ID) 12 Antibodies
Immunoelectrophoresis (IEP) 1 Antibodies
Immunofluorescence (IF) 10 Antibodies
Immunofluorescence (Cultured Cells) (IF (cc)) 1 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 1 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 39 Antibodies
Immunohistochemistry (IHC) 24 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 34 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 36 Antibodies

General

Antigen Complement Component 3 (C3) Antibodies
Reactivity Human
(264), (73), (61), (15), (7), (6), (5), (4), (3), (1), (1), (1)
Host Mouse
(133), (110), (91), (25), (14), (8)
Clonality (Clone)
Monoclonal   ( )
Conjugate This C3 antibody is un-conjugated
(31), (29), (18), (5), (5), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Western Blotting (WB)
(193), (182), (70), (65), (60), (52), (45), (42), (41), (39), (35), (24), (11), (5), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-C3 Antibody

Target Details C3 Application Details Handling References for anti-C3 Antibody (ABIN181105) Images
Specificity This antibody recognises 9 kDa complement component 3a (C3a) which is present in the blood serum. The antibody shows minimal reactivity with other complement proteins in radioimmunoassays and haemagglutination.
Characteristics Synonyms: CPAMD1, Complement component 3, C3 and PZP-like alpha-2-macroglobulindomain-containing protein 1
Purification Affinity Chromatography on Protein A
Immunogen Native purified Human C3a. Genename: C3a
Clone 01-10-21
Isotype IgG1

Target Details C3

Product Details anti-C3 Antibody Application Details Handling References for anti-C3 Antibody (ABIN181105) Images back to top
Antigen
Alternative Name Complement C3 (C3 Antibody Abstract)
Background C3a is generated over the course of complement activation where complement 3 (C3) convertases cleave C3 to C3a and C3b, which is further degraded into iC3b and C3dg/C3d. C3 is crucial in the induction of tolerance generated when an antigen is introduced into immunoprivileged sites and this is exploited by pathogens and cancer cells to evade the immune system by inhibiting complement activation. C3a mediates local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes. C3a is expressed at significantly higher levels in colorectal cancer.Synonyms: C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1, CPAMD1, Complement component 3
Gene ID 718
UniProt P01024
Research Area Complement Factors
Pathways Complement System, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Regulation of G-Protein Coupled Receptor Protein Signaling

Application Details

Product Details anti-C3 Antibody Target Details C3 Handling References for anti-C3 Antibody (ABIN181105) Images back to top
Application Notes Western BLot. ELISA: 1/1500-1/6000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-C3 Antibody Target Details C3 Application Details References for anti-C3 Antibody (ABIN181105) Images back to top
Format Liquid
Concentration 1.0 mg/mL
Buffer Borate buffered saline, pH 8.2 containing 0.09 % Sodium Azide as preservative
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Expiry Date 12 months

References for anti-C3 Antibody (ABIN181105)

Product Details anti-C3 Antibody Target Details C3 Application Details Handling Images back to top
Background publications

Habermann, Roblick, Luke, Prieto, Finlay, Podust, Roman, Oevermann, Schiedeck, Homann, Duchrow, Conrads, Veenstra, Burt, Bruch, Auer, Ried: "Increased serum levels of complement C3a anaphylatoxin indicate the presence of colorectal tumors." in: Gastroenterology, Vol. 131, Issue 4, pp. 1020-9; quiz 1284, 2006

Hugli: "Human anaphylatoxin (C3a) from the third component of complement. Primary structure." in: The Journal of biological chemistry, Vol. 250, Issue 21, pp. 8293-301, 1976