anti-Human C3 antibody for Flow Cytometry

Recommended C3 Antibody (supplied by: Log in to see )

Antigen
Complement Component 3 (C3) Antibodies
  • C3
  • C3-1
  • sb:cb26
  • si:dkey-76b14.4
  • wu:fi34b03
  • wu:fj86e11
  • asp
  • cpamd1
  • AI255234
  • ASP
  • HSE-MSF
  • Plp
  • AHUS5
  • ARMD9
  • C3a
  • C3b
  • CPAMD1
  • C3d
  • c3
  • c3_D
  • complement component c3a
  • complement component 3
  • complement C3-like
  • complement component C3
  • endogenous retrovirus group K, member 13
  • c3a
  • C3
  • c3
  • LOC100060539
  • c3-1
  • ERVK-13
Reactivity
Human
282
79
65
16
9
7
7
4
4
1
1
1
Host
Mouse
139
121
103
27
15
9
Clonality
Monoclonal
Conjugate
This C3 antibody is un-conjugated
33
31
21
6
5
5
4
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
1
1
1
Application
Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
214
196
74
73
67
57
55
50
44
39
38
32
11
5
5
4
3
2
1
1
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN317012
$ 731.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN181107 EIA FACS IHC (fro) WB Mouse IgG1 Log in to see 053A-1149-3-1-4 (1003) 2
1 ABIN181129 EIA FACS IHC (fro) Mouse IgG1 Log in to see 053A-514-3-1-4 2
1 ABIN181106 EIA FACS IHC (fro) WB Mouse IgG1 Log in to see 10-02A 1
1 ABIN951635 EIA FACS WB Chicken Ig Log in to see Polyclonal 1
1 ABIN308050 FACS ELISA FITC Mouse IgG1 Log in to see 6C9
1 ABIN4880587 ELISA FACS ICC IHC WB Mouse IgG1 AA 1521-1649 Log in to see 4C6A8
1 ABIN951634 EIA FACS WB HRP Chicken Ig Log in to see Polyclonal 1
1 ABIN2477804 IHC (fro) FACS ELISA Mouse IgG1 Log in to see 053A-514-3-1-4 2
1 ABIN2477803 IHC (fro) FACS ELISA WB Mouse IgG1 Log in to see 053A-1149-3-1-4 (also known as 1003) 1
1 ABIN308051 FACS ELISA PE Mouse IgG1 Log in to see 6C9
1 ABIN308049 FACS ELISA Biotin Mouse IgG1 Log in to see 6C9
1 ABIN308053 FACS FITC Mouse IgG1 Log in to see 10C7
1 ABIN308052 FACS ELISA Biotin Mouse IgG1 Log in to see 10C7
1 ABIN308054 FACS ELISA PE Mouse IgG1 Log in to see 10C7
1 ABIN4903345 FACS IF WB Mouse IgG Log in to see
1 ABIN601081 IHC (fro) FACS IHC (p) ELISA WB Mouse IgG1 Log in to see
1 ABIN601082 IHC (fro) FACS IHC (p) ELISA Mouse IgG1 Log in to see
1 ABIN212862 FACS ELISA Mouse IgG1 Log in to see 6C9
1 ABIN212863 FACS ELISA Mouse IgG1 Log in to see 10C7
1 ABIN2236866 FACS IHC ELISA WB Mouse IgG1 Log in to see 10B388

General

Antigen Complement Component 3 (C3) Antibodies
Reactivity Human
(282), (79), (65), (16), (9), (7), (7), (4), (4), (1), (1), (1)
Host Mouse
(139), (121), (103), (27), (15), (9)
Clonality (Clone)
Monoclonal   ( )
Conjugate This C3 antibody is un-conjugated
(33), (31), (21), (6), (5), (5), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
(214), (196), (74), (73), (67), (57), (55), (50), (44), (39), (38), (32), (11), (5), (5), (4), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-C3 Antibody

Target Details C3 Application Details Handling References for anti-C3 antibody (ABIN317012) Images
Specificity AM05608PU-N Recognises 42 kDa inactive complement component 3b (iC3b) neoantigen present in blood serum.
Cross-Reactivity (Details) Species reactivity (tested):Human.
Characteristics Synonyms: CPAMD1, Complement component 3, C3 and PZP-like alpha-2-macroglobulindomain-containing protein 1
Purification Purified IgG prepared by affinity chromatography on Protein A
Immunogen Purified Human iC3b
Clone 013III-1-16
Isotype IgG2b

Target Details C3

Product Details anti-C3 Antibody Application Details Handling References for anti-C3 antibody (ABIN317012) Images back to top
Antigen
Alternative Name Complement C3 (C3 Antibody Abstract)
Background Cleavage of C3b into iC3b by the protease factor I, in the presence of cofactors, changes the structure and resulting binding properties, preventing binding by complement factor B and properdin. This prevents further progression of the complement pathway, providing another mechanism by which the complement pathway may be regulated. iC3b is thought to play a role in inducing tolerance, binding complement receptor type 3 on antigen presenting cells and stimulating them to produce transforming growth factor beta2 and interleukin-10. It is thought to stimulate B-cells via the CD21 receptor providing a link between the innate and adaptive immune responses. Additionally iC3b acts as an opsonin which is recognised by complement receptor 3 expressed on leukocytes or complement receptor of the immunoglobulin superfamily (CRIg) on Kupffer cells, stimulating phagocytosis of the tagged cells. iC3b levels are often elevated in diseases such as Systemic Lupus Erythematosis and Rheumatoid arthritis.Synonyms: C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1, CPAMD1, Complement component 3
Gene ID 718
UniProt P01024
Research Area Complement Factors
Pathways Complement System, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Regulation of G-Protein Coupled Receptor Protein Signaling

Application Details

Product Details anti-C3 Antibody Target Details C3 Handling References for anti-C3 antibody (ABIN317012) Images back to top
Application Notes ELISA. Western blot. Flow cytometry. Immunohistochemistry on Frozen Sections. Histology Positive Control Tissue: Kidney from post streptoccal glomerulonephritispatients.
Restrictions For Research Use only

Handling

Product Details anti-C3 Antibody Target Details C3 Application Details References for anti-C3 antibody (ABIN317012) Images back to top
Concentration 1.0 mg/mL
Buffer Borate buffered saline pH 8.2-8.4, 0.09 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Expiry Date 12 months

References for anti-C3 antibody (ABIN317012)

Product Details anti-C3 Antibody Target Details C3 Application Details Handling Images back to top
Background publications

Janssen, Gros: "Structural insights into the central complement component C3." in: Molecular immunology, Vol. 44, Issue 1-3, pp. 3-10, 2006

Bergmann-Leitner, Leitner, Tsokos: "Complement 3d: from molecular adjuvant to target of immune escape mechanisms." in: Clinical immunology (Orlando, Fla.), Vol. 121, Issue 2, pp. 177-85, 2006

Sohn, Bora, Suk, Molina, Kaplan, Bora: "Tolerance is dependent on complement C3 fragment iC3b binding to antigen-presenting cells." in: Nature medicine, Vol. 9, Issue 2, pp. 206-12, 2003