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|Antigen||Complement Component 3 (C3) Antibodies|
|Reactivity||Mouse (Murine) Alternatives|
|Conjugate||This C3 antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Western Blotting (WB)
|2 references available|
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Product Details anti-C3 AntibodyTarget Details C3 Application Details Handling References for anti-C3 antibody (ABIN114403) Images
|Cross-Reactivity (Details)||Species reactivity (tested):Mouse.|
|Purification||Protein G Chromatography.|
|Immunogen||C57BL/6 thymocytes incubated with a rat IgG2b anti-murine Thy-1 (RmT1) and C57BL/6 serum (as a source for C1q). Donor: Lou/c rat spleen cells. Fusion Partner: Mouse myeloma cell line P3X63 Ag. 8.653.|
Target Details C3Product Details anti-C3 Antibody Application Details Handling References for anti-C3 antibody (ABIN114403) Images back to top
|Alternative Name||Complement C3 (C3 Antibody Abstract)|
|Background||C3 is the most abundant complement protein in serum. C3 and its cleavage products, C3a and C3b, play a central role in the complement activation cascade. C3b forms an integral part of the C3 and C5 convertases as it promotes complement activation and the subsequent formation of the membrane attack complex. C3a possesses anaphlatoxic as well as various immunoregulatory properties. Also, C3 has been implicated in developmental and non-imflammatory process such as hematopoiesis, skeletal and vascular development and reproduction.Synonyms: C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1, CPAMD1, Complement component 3|
|Research Area||Complement Factors|
|Pathways||Complement System, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Regulation of G-Protein Coupled Receptor Protein Signaling|
Application DetailsProduct Details anti-C3 Antibody Target Details C3 Handling References for anti-C3 antibody (ABIN114403) Images back to top
ELISA. Western blot. Flow Cytometry. Immunofluorescence. Immunohistochemistry on Acetone Fixed Frozen Sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Protocol||FLOW CYTOMETRY ANALYSIS: 1. Prepare cell suspension in Media A. For cell preparations, deplete the red blood cellpopulation with cell separation medium . 2. Wash 2 times. 3. Resuspend cells to 1x10e6 cells in approximately 50 µl Media A in a microcentrifugetube, i. e. 50 µl of cells resuspended to 2x10e7 cells/ml (the contents of 1 tube = 1 test). 4. To each tube add 0. 5 µg of ABIN114403 per 10e6 cells. 5. Vortex the tubes to ensure thorough mixing of antibody and cells. 6. Incubate the tubes for 30 minutes at 4°C. 7. Wash 2 times at 4°C. 8. Add 100 µl of secondary antibody (FITC Goat anti-rat IgG (H+L)) at appropriate dilution. 9. Incubate tubes at 4°C for 30-60 minutes. (It is recommended that the tubes areprotected from light since most fluorochromes are light sensitive). 10. Wash 2 times at 4°C in Media B. 11. Resuspend the cell pellet in 50 µl ice cold Media B. 12. Transfer to suitable tubes for flow cytometric analysis containing 15 µl of propidiumiodide at 0. 5 mg/ml in PBS. (This stains dead cells by intercalating DNA). Media: A. Phosphate buffered saline (pH 7. 2) + 5% normal serum of host species + sodium azide(100 µl of 2M sodium azide in 100 mls). B. Phosphate buffered saline (pH 7. 2) + 0. 5% Bovine serum albumin + sodium azide (100µl of 2M sodium azide in 100 mls).|
|Restrictions||For Research Use only|
HandlingProduct Details anti-C3 Antibody Target Details C3 Application Details References for anti-C3 antibody (ABIN114403) Images back to top
|Buffer||PBS containing 0.02 % Sodium Azide as preservative.|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.|
References for anti-C3 antibody (ABIN114403)Product Details anti-C3 Antibody Target Details C3 Application Details Handling Images back to top
Mastellos, Prechl, László, Papp, Oláh, Argyropoulos, Franchini, Tudoran, Markiewski, Lambris, Erdei: "Novel monoclonal antibodies against mouse C3 interfering with complement activation: description of fine specificity and applications to various immunoassays." in: Molecular immunology, Vol. 40, Issue 16, pp. 1213-21, 2004
Kremmer, Thierfelder, Felber, Hoffmann-Fezer, Wasiliu: "Monoclonal antibodies to complement components without the need of their prior purification. II. Antibodies to mouse C3 and C4." in: Hybridoma, Vol. 9, Issue 4, pp. 309-17, 1990
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