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anti-Mouse (Murine) C3 antibody for Immunodiffusion

Recommended C3 Antibody (supplied by: Log in to see )

Antigen
Complement Component 3 (C3) Antibodies
  • AHUS5
  • AI255234
  • ARMD9
  • asp
  • ASP
  • c3
  • C3
  • C3-1
  • C3a
  • C3b
  • C3d
  • c3_D
  • CPAMD1
  • cpamd1
  • HSE-MSF
  • Plp
  • sb:cb26
  • si:dkey-76b14.4
  • wu:fi34b03
  • wu:fj86e11
Reactivity
Mouse (Murine)
243
64
55
18
13
5
5
5
3
2
1
Host
Goat
141
102
85
24
13
7
Clonality
Polyclonal
Conjugate
This C3 antibody is conjugated to HRP
34
31
19
6
6
5
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
1
Application
Enzyme Immunoassay (EIA), Immunodiffusion (ID), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
193
187
70
67
58
45
44
42
39
34
33
24
20
5
4
1
1
1
1
1
1
Supplier
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Catalog No. ABIN783940
$ 496.65
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.00087119907 ABIN783939 ID IHC (fro) IF IP FITC Goat IgG Log in to see Polyclonal
0.00087119907 ABIN783938 EIA ID IHC (fro) IF IP WB Biotin Goat IgG Log in to see Polyclonal
0.00087119907 ABIN783937 EIA ID IHC (fro) IF IP Goat Log in to see Polyclonal
0.00087119907 ABIN783941 ID IP WB Goat Log in to see Polyclonal

Similar anti-C3 Antibodies

Application / Reactivity Mouse (Murine)
ELISA 20 Antibodies
Enzyme Immunoassay (EIA) 4 Antibodies
Flow Cytometry (FACS) 20 Antibodies
Immunoassay (IA) 17 Antibodies
Immunocytochemistry (ICC) 18 Antibodies
Immunodiffusion (ID) 5 Antibodies
Immunofluorescence (IF) 23 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 21 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 29 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 21 Antibodies
Immunoprecipitation (IP) 24 Antibodies
Western Blotting (WB) 42 Antibodies

General

Antigen Complement Component 3 (C3) Antibodies
Reactivity Mouse (Murine)
(243), (64), (55), (18), (13), (5), (5), (5), (3), (2), (1)
Host Goat
(141), (102), (85), (24), (13), (7)
Clonality
Polyclonal
Conjugate This C3 antibody is conjugated to HRP
(34), (31), (19), (6), (6), (5), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (1)
Application Enzyme Immunoassay (EIA), Immunodiffusion (ID), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
(193), (187), (70), (67), (58), (45), (44), (42), (39), (34), (33), (24), (20), (5), (4), (1), (1), (1), (1), (1), (1)
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Product Details anti-C3 Antibody

Target Details C3 Application Details Handling Images
Specificity In Immunoelectrophoresis against fresh Mouse serum, a single precipitin line is obtained in the beta-1 region representing native C3. Against serum containing partly activated C3, a precipitin line is obtained which extends from the beta-1 into the alpha-2 region, demonstrating a gradient. In old serum containing totally activated C3 a single precipitin line in the alpha-2 region is obtained. Antisera to C3c cab also react with the fragments C3b, C3bi and smaller fragments, since they all carry antigenic determinants of the C3c domain. The product does not react with any other protein components of Mouse serum or plasma. Cross-reactivity: The antiserum does not cross-react with any other component of Mouse plasma. Inter-species cross-reactivity is a normal feature of antibodies to plasma proteins since they frequently share antigenic determinants. Cross-reactivity of this antiserum has not been tested in detail.
Characteristics Molar Ratio: Peroxidase/IgG ~1.7
Purification The IgG fraction is isolated and purified from the antiserum and contains the bulk of the defined antibody specificity. It is free of other serum proteins as tested by immunoelectrophoresis and double radial immunodiffusion.
Immunogen C3c isolated and purified from pooled normal Mouse serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Isotype IgG

Target Details C3

Product Details anti-C3 Antibody Application Details Handling Images back to top
Antigen
Alternative Name Complement C3 (C3 Antibody Abstract)
Background C3 is the most abundant complement protein in rat serum. Its biological function strongly resembles that of C3 in man and other laboratory animal species. It has a central role in the activation system being common to both pathways. Activation of C3 is achieved by very specific limited proteolysis resulting in the release of a number of degradation fragments. The anaphylotoxin C3a promotes smooth muscle contraction and increases vascular permeability: the large C3b fragment is involved in binding to the complement activator and can be interact with specific receptors to allow efficient clearance of the activating cell or particle, degradation fragments of C3b (C3bi, C3c, C3dg C3d) are important in receptor binding and clearance mechanisms, in virus neutralization and possibly in the immune response.Synonyms: CPAMD1, Complement component 3
Gene ID 24232
NCBI Accession NP_058690
Research Area Complement Factors
Pathways Complement System

Application Details

Product Details anti-C3 Antibody Target Details C3 Handling Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-C3 Antibody Target Details C3 Application Details Images back to top
Format Liquid
Reconstitution Restore by adding 1 mL of sterile distilled water.
Concentration 10.0 mg/mL
Buffer PBS, pH 7.2 without preservatives
Preservative Without preservative
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Prior to reconstitution store at 2-8 °C. Following reconstitution store undiluted at 2-8 °C for one week or (in aliquots) at -20 °C for longer.