anti-Mouse (Murine) C3 antibody for Immunodiffusion

Recommended C3 Antibody (supplied by: Log in to see )

Antigen
Complement Component 3 (C3) Antibodies
  • C3
  • C3-1
  • sb:cb26
  • si:dkey-76b14.4
  • wu:fi34b03
  • wu:fj86e11
  • asp
  • cpamd1
  • AI255234
  • ASP
  • HSE-MSF
  • Plp
  • AHUS5
  • ARMD9
  • C3a
  • C3b
  • CPAMD1
  • C3d
  • c3
  • c3_D
  • complement component c3a
  • complement component 3
  • complement C3-like
  • complement component C3
  • endogenous retrovirus group K, member 13
  • c3a
  • C3
  • c3
  • LOC100060539
  • c3-1
  • ERVK-13
Reactivity
Mouse (Murine)
282
77
64
16
9
7
7
4
4
1
1
1
Host
Goat
140
120
102
27
15
9
Clonality
Polyclonal
Conjugate
This C3 antibody is conjugated to FITC
33
30
21
6
5
5
4
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
1
1
1
Application
Immunodiffusion (ID), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunoprecipitation (IP)
214
196
74
72
67
56
56
49
45
39
37
32
11
5
5
4
3
2
1
1
1
1
1
1
1
1
1
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Catalog No. ABIN783939
$ 404.25
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN783940 EIA ID IHC (fro) IF IP WB HRP Goat IgG Log in to see Polyclonal
1 ABIN783938 EIA ID IHC (fro) IF IP WB Biotin Goat IgG Log in to see Polyclonal
1 ABIN783937 EIA ID IHC (fro) IF IP Goat Log in to see Polyclonal
1 ABIN5573350 ELISA ID WB Goat Log in to see Polyclonal
1 ABIN783941 ID IP WB Goat Log in to see Polyclonal

General

Antigen Complement Component 3 (C3) Antibodies
Reactivity Mouse (Murine)
(282), (77), (64), (16), (9), (7), (7), (4), (4), (1), (1), (1)
Host Goat
(140), (120), (102), (27), (15), (9)
Clonality
Polyclonal
Conjugate This C3 antibody is conjugated to FITC
(33), (30), (21), (6), (5), (5), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Application Immunodiffusion (ID), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunoprecipitation (IP)
(214), (196), (74), (72), (67), (56), (56), (49), (45), (39), (37), (32), (11), (5), (5), (4), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
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Product Details anti-C3 Antibody

Target Details C3 Application Details Handling Images
Specificity In Immunoelectrophoresis against fresh Mouse serum, a single precipitin line is obtained in the beta-1 region representing native C3. Against serum containing partly activated C3, a precipitin line is obtained which extends from the beta-1 into the alpha-2 region, demonstrating a gradient. In old serum containing totally activated C3 a single precipitin line in the alpha-2 region is obtained. Antisera to C3c cab also react with the fragments C3b, C3bi and smaller fragments, since they all carry antigenic determinants of the C3c domain. The product does not react with any other proteins component of Mouse serum or plasma. Cross-reactivity: The antiserum does not cross-react with any other component of Mouse plasma. Inter-species cross-reactivity is a normal feature of antibodies to plasma proteins since they frequently share antigenic determinants. Cross-reactivity of this antiserum has not been tested in detail.
Characteristics Absorption / Emission: 492 nm / 515 nm
Molar Ratio: Fluorochrome/IgG ~1.9
Purification The IgG fraction is isolated and purified from the antiserum and contains the bulk of the defined antibody specificity. It is free of other serum proteins as tested by Immunoelectrophoresis and Double Radial Immunodiffusion
Immunogen C3c isolated and purified from pooled normal Mouse serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Isotype IgG

Target Details C3

Product Details anti-C3 Antibody Application Details Handling Images back to top
Antigen
Alternative Name Complement C3 (C3 Antibody Abstract)
Background C3 is the most abundant complement protein in rat serum. Its biological function strongly resembles that of C3 in man and other laboratory animal species. It has a central role in the activation system being common to both pathways. Activation of C3 is achieved by very specific limited proteolysis resulting in the release of a number of degradation fragments. The anaphylotoxin C3a promotes smooth muscle contraction and increases vascular permeability: the large C3b fragment is involved in binding to the complement activator and can be interact with specific receptors to allow efficient clearance of the activating cell or particle, degradation fragments of C3b (C3bi, C3c, C3dg C3d) are important in receptor binding and clearance mechanisms, in virus neutralization and possibly in the immune response.Synonyms: CPAMD1, Complement component 3
Gene ID 24232
NCBI Accession NP_058690
Research Area Complement Factors
Pathways Complement System, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Regulation of G-Protein Coupled Receptor Protein Signaling

Application Details

Product Details anti-C3 Antibody Target Details C3 Handling Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-C3 Antibody Target Details C3 Application Details Images back to top
Format Liquid
Reconstitution Restore by adding 1 mL of sterile distilled water
Concentration 6.5 mg/mL
Buffer PBS, pH 7.2 without preservatives
Preservative Without preservative
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Prior to reconstitution store at 2-8 °C. Following reconstitution store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.