anti-Mouse (Murine) C3 antibody for Immunohistochemistry (Frozen Sections)

Recommended C3 Antibody (supplied by: Log in to see )

Antigen
Complement Component 3 (C3) Antibodies
  • C3
  • C3-1
  • sb:cb26
  • si:dkey-76b14.4
  • wu:fi34b03
  • wu:fj86e11
  • asp
  • cpamd1
  • AI255234
  • ASP
  • HSE-MSF
  • Plp
  • AHUS5
  • ARMD9
  • C3a
  • C3b
  • CPAMD1
  • C3d
  • c3
  • c3_D
  • complement component c3a
  • complement component 3
  • complement C3-like
  • complement component C3
  • endogenous retrovirus group K, member 13
  • c3a
  • C3
  • c3
  • LOC100060539
  • c3-1
  • ERVK-13
Reactivity
Mouse (Murine)
279
77
64
16
9
7
7
4
4
1
1
1
Host
Rat
139
119
102
26
15
9
Clonality
Monoclonal
Conjugate
This C3 antibody is un-conjugated
33
31
21
6
5
5
4
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
1
1
1
Application
Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Western Blotting (WB)
210
195
74
72
67
57
54
48
44
39
38
32
11
5
5
4
3
2
1
1
1
1
1
1
1
1
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Available images

Catalog No. ABIN114403
$ 522.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN681913 IHC (fro) IF (p) IHC (p) Rabbit IgG AA 1611-1631 Log in to see Polyclonal 2
1 ABIN152429 CyTOF FACS IA ICC IF IHC IHC (fro) IHC (p) IP WB Rat IgG2a Log in to see 11H9 1
1 ABIN681922 IHC (fro) IHC (p) HRP Rabbit IgG AA 1611-1631 Log in to see Polyclonal
1 ABIN783940 EIA ID IHC (fro) IF IP WB HRP Goat IgG Log in to see Polyclonal
1 ABIN783939 ID IHC (fro) IF IP FITC Goat IgG Log in to see Polyclonal
1 ABIN681915 IHC (fro) IHC (p) Biotin Rabbit IgG AA 1611-1631 Log in to see Polyclonal
1 ABIN200615 FACS IHC (fro) IP ELISA WB Rat IgG2a Log in to see 11H9
1 ABIN4299771 CyTOF FACS IA ICC IF IHC IHC (fro) IHC (p) IP WB Rat IgG2a Log in to see 11H9
1 ABIN783938 EIA ID IHC (fro) IF IP WB Biotin Goat IgG Log in to see Polyclonal
1 ABIN1106780 IHC (fro) WB Rabbit Ig Log in to see Polyclonal
1 ABIN200616 IHC (fro) WB Rabbit IgG Log in to see Polyclonal
1 ABIN4299773 FACS ICC IF IHC (fro) IHC (p) WB Alexa Fluor 488 Rat IgG2a Log in to see 11H9
1 ABIN4299783 FACS ICC IF IHC (fro) IHC (p) WB DyLight 680 Rat IgG2a Log in to see 11H9
1 ABIN4299781 FACS ICC IF IHC (fro) IHC (p) WB DyLight 550 Rat IgG2a Log in to see 11H9
1 ABIN4299774 FACS ICC IF IHC (fro) IHC (p) WB Alexa Fluor 647 Rat IgG2a Log in to see 11H9
1 ABIN4299778 FACS ICC IF IHC (fro) IHC (p) WB DyLight 405 Rat IgG2a Log in to see 11H9
1 ABIN4299782 FACS ICC IF IHC (fro) IHC (p) WB DyLight 650 Rat IgG2a Log in to see 11H9
1 ABIN4299777 FACS ICC IF IHC (fro) IHC (p) WB DyLight 350 Rat IgG2a Log in to see 11H9
1 ABIN4299776 ICC IF IHC (fro) IHC (p) WB Biotin Rat IgG2a Log in to see 11H9
1 ABIN4299780 FACS ICC IF IHC (fro) IHC (p) WB DyLight 488 Rat IgG2a Log in to see 11H9

Top referenced anti-C3 antibody for Immunohistochemistry (Frozen Sections)

Similar anti-C3 Antibodies

Application / Reactivity Mouse (Murine)
Cytometry by Time of Flight (CyTOF) 2 Antibodies
ELISA 23 Antibodies
Enzyme Immunoassay (EIA) 4 Antibodies
Flow Cytometry (FACS) 21 Antibodies
Immunoassay (IA) 4 Antibodies
Immunocytochemistry (ICC) 18 Antibodies
Immunodiffusion (ID) 6 Antibodies
Immunofluorescence (IF) 24 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 1 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 13 Antibodies
Immunohistochemistry (IHC) 14 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 28 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 21 Antibodies
Immunoprecipitation (IP) 15 Antibodies
Immunosubtraction (ISub) 1 Antibodies
Simple Western (SimWes) 1 Antibodies
Western Blotting (WB) 53 Antibodies

General

Antigen Complement Component 3 (C3) Antibodies
Reactivity Mouse (Murine)
(279), (77), (64), (16), (9), (7), (7), (4), (4), (1), (1), (1)
Host Rat
(139), (119), (102), (26), (15), (9)
Clonality (Clone)
Monoclonal   ( )
Conjugate This C3 antibody is un-conjugated
(33), (31), (21), (6), (5), (5), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Western Blotting (WB)
(210), (195), (74), (72), (67), (57), (54), (48), (44), (39), (38), (32), (11), (5), (5), (4), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 2 references available
Supplier Log in to see

Product Details anti-C3 Antibody

Target Details C3 Application Details Handling ProductDetails: References for anti-C3 antibody (ABIN114403) Images
Cross-Reactivity (Details) Species reactivity (tested):Mouse.
Purification Protein G Chromatography.
Immunogen C57BL/6 thymocytes incubated with a rat IgG2b anti-murine Thy-1 (RmT1) and C57BL/6 serum (as a source for C1q). Donor: Lou/c rat spleen cells. Fusion Partner: Mouse myeloma cell line P3X63 Ag. 8.653.
Clone RMC11H9
Isotype IgG2a

Target Details C3

Product Details anti-C3 Antibody Application Details Handling ProductDetails: References for anti-C3 antibody (ABIN114403) Images back to top
Antigen
Alternative Name Complement C3 (C3 Antibody Abstract)
Background C3 is the most abundant complement protein in serum. C3 and its cleavage products, C3a and C3b, play a central role in the complement activation cascade. C3b forms an integral part of the C3 and C5 convertases as it promotes complement activation and the subsequent formation of the membrane attack complex. C3a possesses anaphlatoxic as well as various immunoregulatory properties. Also, C3 has been implicated in developmental and non-imflammatory process such as hematopoiesis, skeletal and vascular development and reproduction.Synonyms: C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1, CPAMD1, Complement component 3
Gene ID 10090
UniProt P01027
Research Area Complement Factors
Pathways Complement System, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Regulation of G-Protein Coupled Receptor Protein Signaling

Application Details

Product Details anti-C3 Antibody Target Details C3 Handling ProductDetails: References for anti-C3 antibody (ABIN114403) Images back to top
Application Notes ELISA. Western blot. Flow Cytometry. Immunofluorescence. Immunohistochemistry on Acetone Fixed Frozen Sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Protocol FLOW CYTOMETRY ANALYSIS: 1. Prepare cell suspension in Media A. For cell preparations, deplete the red blood cellpopulation with cell separation medium . 2. Wash 2 times. 3. Resuspend cells to 1x10e6 cells in approximately 50 µl Media A in a microcentrifugetube, i. e. 50 µl of cells resuspended to 2x10e7 cells/ml (the contents of 1 tube = 1 test). 4. To each tube add 0. 5 µg of ABIN114403 per 10e6 cells. 5. Vortex the tubes to ensure thorough mixing of antibody and cells. 6. Incubate the tubes for 30 minutes at 4°C. 7. Wash 2 times at 4°C. 8. Add 100 µl of secondary antibody (FITC Goat anti-rat IgG (H+L)) at appropriate dilution. 9. Incubate tubes at 4°C for 30-60 minutes. (It is recommended that the tubes areprotected from light since most fluorochromes are light sensitive). 10. Wash 2 times at 4°C in Media B. 11. Resuspend the cell pellet in 50 µl ice cold Media B. 12. Transfer to suitable tubes for flow cytometric analysis containing 15 µl of propidiumiodide at 0. 5 mg/ml in PBS. (This stains dead cells by intercalating DNA). Media: A. Phosphate buffered saline (pH 7. 2) + 5% normal serum of host species + sodium azide(100 µl of 2M sodium azide in 100 mls). B. Phosphate buffered saline (pH 7. 2) + 0. 5% Bovine serum albumin + sodium azide (100µl of 2M sodium azide in 100 mls).
Restrictions For Research Use only

Handling

Product Details anti-C3 Antibody Target Details C3 Application Details ProductDetails: References for anti-C3 antibody (ABIN114403) Images back to top
Concentration 1.0 mg/mL
Buffer PBS containing 0.02 % Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

ProductDetails: References for anti-C3 antibody (ABIN114403)

Product Details anti-C3 Antibody Target Details C3 Application Details Handling Images back to top
Background publications

Mastellos, Prechl, László, Papp, Oláh, Argyropoulos, Franchini, Tudoran, Markiewski, Lambris, Erdei: "Novel monoclonal antibodies against mouse C3 interfering with complement activation: description of fine specificity and applications to various immunoassays." in: Molecular immunology, Vol. 40, Issue 16, pp. 1213-21, 2004

Kremmer, Thierfelder, Felber, Hoffmann-Fezer, Wasiliu: "Monoclonal antibodies to complement components without the need of their prior purification. II. Antibodies to mouse C3 and C4." in: Hybridoma, Vol. 9, Issue 4, pp. 309-17, 1990

Images

Product Details anti-C3 Antibody Target Details C3 Application Details Handling ProductDetails: References for anti-C3 antibody (ABIN114403) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-C3 antibody (Complement Component 3) (ABIN114403) Representative histogram of thymocytes stained with ABIN114403-FITC label