anti-Mouse (Murine) C3 antibody for Simple Western

Recommended C3 Antibody (supplied by: Log in to see )

Antigen
Complement Component 3 (C3) Antibodies
  • C3
  • C3-1
  • sb:cb26
  • si:dkey-76b14.4
  • wu:fi34b03
  • wu:fj86e11
  • asp
  • cpamd1
  • AI255234
  • ASP
  • HSE-MSF
  • Plp
  • AHUS5
  • ARMD9
  • C3a
  • C3b
  • CPAMD1
  • C3d
  • c3
  • c3_D
  • complement component c3a
  • complement component 3
  • complement C3-like
  • complement component C3
  • endogenous retrovirus group K, member 13
  • c3a
  • C3
  • c3
  • LOC100060539
  • c3-1
  • ERVK-13
Epitope
C-Term
15
15
6
6
5
5
5
4
3
3
3
2
2
2
2
2
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
275
72
61
15
7
6
5
4
4
1
1
1
Host
Rabbit
140
115
91
25
14
8
Clonality
Polyclonal
Conjugate
This C3 antibody is un-conjugated
32
31
18
6
5
5
4
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Simple Western (SimWes), Western Blotting (WB)
196
183
69
66
65
54
51
47
44
39
35
29
11
5
5
4
3
2
1
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN442056
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Application / Reactivity Mouse (Murine)
Cytometry by Time of Flight (CyTOF) 2 Antibodies
ELISA 20 Antibodies
Enzyme Immunoassay (EIA) 4 Antibodies
Flow Cytometry (FACS) 19 Antibodies
Immunoassay (IA) 4 Antibodies
Immunocytochemistry (ICC) 16 Antibodies
Immunodiffusion (ID) 5 Antibodies
Immunofluorescence (IF) 22 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 1 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 13 Antibodies
Immunohistochemistry (IHC) 11 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 26 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 19 Antibodies
Immunoprecipitation (IP) 14 Antibodies
Immunosubtraction (ISub) 1 Antibodies
Simple Western (SimWes) 1 Antibodies
Western Blotting (WB) 48 Antibodies

General

Antigen Complement Component 3 (C3) Antibodies
Epitope C-Term
(15), (15), (6), (6), (5), (5), (5), (4), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(275), (72), (61), (15), (7), (6), (5), (4), (4), (1), (1), (1)
Host Rabbit
(140), (115), (91), (25), (14), (8)
Clonality
Polyclonal
Conjugate This C3 antibody is un-conjugated
(32), (31), (18), (6), (5), (5), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Simple Western (SimWes), Western Blotting (WB)
(196), (183), (69), (66), (65), (54), (51), (47), (44), (39), (35), (29), (11), (5), (5), (4), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1)
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Product Details anti-C3 Antibody

Target Details C3 Application Details Handling Images
Purification Immunogen affinity purified
Immunogen Recombinant protein encompassing a sequence within the C-terminus region of human C3. The exact sequence is proprietary.
Isotype IgG

Target Details C3

Product Details anti-C3 Antibody Application Details Handling Images back to top
Antigen
Alternative Name Complement C3 (C3 Antibody Abstract)
Background Gene Symbol: C3
Molecular Weight Theoretical MW: 187 kDa
Gene ID 718
UniProt P01024
Research Area Complement Factors
Pathways Complement System, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Regulation of G-Protein Coupled Receptor Protein Signaling

Application Details

Product Details anti-C3 Antibody Target Details C3 Handling Images back to top
Application Notes Western Blot 1:500-1:3000, Simple Western 1:50, Immunocytochemistry/Immunofluorescence 1:100-1:1000, Immunoprecipitation 1:100-1:500, Immunohistochemistry-Paraffin 1:100-1:1000Immunosubtraction. In Simple Western only 10 - 15 μL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product Details anti-C3 Antibody Target Details C3 Application Details Images back to top
Format Liquid
Concentration 0.16 mg/mL
Buffer PBS ( pH 7.0) and 20 % Glycerol
Buffer contains: 0.025 % Proclin 300
Preservative ProClin
Precaution of Use This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage -80 °C,-20 °C
Storage Comment Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.

Images

Product Details anti-C3 Antibody Target Details C3 Application Details Handling back to top
Supplier Images
Immunoprecipitation (IP) image for anti-C3 antibody (Complement Component 3) (C-Term) (ABIN442056) Immunoprecipitation: Complement C3 Antibody [NBP1-32080] - Immunoprecipitation of C3 ...
Simple Western (SimWes) image for anti-C3 antibody (Complement Component 3) (C-Term) (ABIN442056) Simple Western: Complement C3 Antibody [NBP1-32080] - Simple Western lane view shows ...
Immunofluorescence (IF) image for anti-C3 antibody (Complement Component 3) (C-Term) (ABIN442056) Immunocytochemistry/Immunofluorescence: Complement C3 Antibody [NBP1-32080] - HeLa ce...
Western Blotting (WB) image for anti-C3 antibody (Complement Component 3) (C-Term) (ABIN442056) Western Blot: Complement C3 Antibody [NBP1-32080] - A: Non-transfected 293T lysates B...
Western Blotting (WB) image for anti-C3 antibody (Complement Component 3) (C-Term) (ABIN442056) Western Blot: Complement C3 Antibody [NBP1-32080] - Lane A: Non-transfected 293T lysa...
Western Blotting (WB) image for anti-C3 antibody (Complement Component 3) (C-Term) (ABIN442056) Western Blot: Complement C3 Antibody [NBP1-32080] - A:Hep G2.
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-C3 antibody (Complement Component 3) (C-Term) (ABIN442056) Immunohistochemistry-Paraffin: Complement C3 Antibody [NBP1-32080] - Paraffin-embedde...
Western Blotting (WB) image for anti-C3 antibody (Complement Component 3) (C-Term) (ABIN442056) Western Blot: Complement C3 Antibody [NBP1-32080] - A. 293T whole cell lysate/extract...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-C3 antibody (Complement Component 3) (C-Term) (ABIN442056) Immunohistochemistry-Paraffin: Complement C3 Antibody [NBP1-32080] - Paraffin-embedde...
Immunofluorescence (IF) image for anti-C3 antibody (Complement Component 3) (C-Term) (ABIN442056) Immunocytochemistry/Immunofluorescence: Complement C3 Antibody [NBP1-32080] - Parafor...
Western Blotting (WB) image for anti-C3 antibody (Complement Component 3) (C-Term) (ABIN442056) Western Blot: Complement C3 Antibody [NBP1-32080] - Various whole cell extracts (30 u...