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anti-Rat (Rattus) C3 antibody for Immunofluorescence

Recommended C3 Antibody (supplied by: Log in to see )

Antigen
Complement Component 3 (C3) Antibodies
  • AHUS5
  • AI255234
  • ARMD9
  • asp
  • ASP
  • c3
  • C3
  • C3-1
  • C3a
  • C3b
  • C3d
  • c3_D
  • CPAMD1
  • cpamd1
  • HSE-MSF
  • Plp
  • sb:cb26
  • si:dkey-76b14.4
  • wu:fi34b03
  • wu:fj86e11
Reactivity
Rat (Rattus)
246
67
55
18
13
5
5
5
3
2
1
Host
Goat
143
103
85
25
13
8
Clonality
Polyclonal
Conjugate
This C3 antibody is conjugated to FITC
34
30
20
6
6
5
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
1
Application
Immunodiffusion (ID), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunoprecipitation (IP)
195
192
71
70
60
47
46
43
41
34
33
26
22
5
4
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN568526
$ 392.70
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0010840179 ABIN568527 EIA ID IHC (fro) IF IP WB HRP Goat IgG Log in to see Polyclonal
0.0010840179 ABIN568525 EIA ID IHC (fro) IF IP WB Biotin Goat IgG Log in to see Polyclonal
0.0010840179 ABIN1173828 ICC IF IHC WB FITC Rabbit IgG Log in to see Polyclonal
0.0010840179 ABIN568524 EIA ID IHC (fro) IF IP Goat IgG Log in to see Polyclonal

Similar anti-C3 Antibodies

Application / Reactivity Rat (Rattus)
ELISA 34 Antibodies
Enzyme Immunoassay (EIA) 4 Antibodies
Immunoassay (IA) 1 Antibodies
Immunocytochemistry (ICC) 2 Antibodies
Immunodiffusion (ID) 5 Antibodies
Immunofluorescence (IF) 5 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 9 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 8 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 24 Antibodies
Immunoprecipitation (IP) 6 Antibodies
Western Blotting (WB) 11 Antibodies

General

Antigen Complement Component 3 (C3) Antibodies
Reactivity Rat (Rattus)
(246), (67), (55), (18), (13), (5), (5), (5), (3), (2), (1)
Host Goat
(143), (103), (85), (25), (13), (8)
Clonality
Polyclonal
Conjugate This C3 antibody is conjugated to FITC
(34), (30), (20), (6), (6), (5), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (1)
Application Immunodiffusion (ID), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunoprecipitation (IP)
(195), (192), (71), (70), (60), (47), (46), (43), (41), (34), (33), (26), (22), (5), (4), (1), (1), (1), (1), (1), (1), (1)
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Product Details anti-C3 Antibody

Target Details C3 Application Details Handling Images
Specificity In Immunoelectrophoresis against fresh Rat serum, a single precipitin line is obtained in the beta-1 region representing native C3. Against serum containing partly activated C3, a precipitin line is obtained which extends from the beta-1 into the alpha-2 region, demonstrating a gradient. In old serum containing totally activated C3 a single precipitin line in the alpha-2 region is obtained. Antisera to C3c cab also react with the fragments C3b, C3bi and smaller fragments, since they all carry antigenic determinants of the C3c domain. The product does not react with any other protein components of Rat serum or plasma. Cross-reactivity: The antiserum does not cross-react with any other component of Rat plasma. Inter-species cross-reactivity is a normal feature of antibodies to plasma proteins since they frequently share antigenic determinants. Cross-reactivity of this antiserum has not been tested in detail. Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies cross-reacting with other with other plasma proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.
Characteristics Absorption / Emission: 492 nm / 515 nm
Molar Ratio: Fluorescein/IgG ~1.8
Purification The IgG fraction is isolated and purified from the antiserum and contains the bulk of the defined antibody specificity. It is free of other serum proteins as tested by immunoelectrophoresis and double radial immunodiffusion
Immunogen C3c isolated and purified from pooled normal Rat serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Isotype IgG

Target Details C3

Product Details anti-C3 Antibody Application Details Handling Images back to top
Antigen
Alternative Name Complement C3 (C3 Antibody Abstract)
Background C3 is the most abundant complement protein in rat serum. Its biological function strongly resembles that of C3 in man and other laboratory animal species. It has a central role in the activation system being common to both pathways. Activation of C3 is achieved by very specific limited proteolysis resulting in the release of a number of degradation fragments. The anaphylotoxin C3a promotes smooth muscle contraction and increases vascular permeability: the large C3b fragment is involved in binding to the complement activator and can be interact with specific receptors to allow efficient clearance of the activating cell or particle, degradation fragments of C3b (C3bi, C3c, C3dg C3d) are important in receptor binding and clearance mechanisms, in virus neutralization and possibly in the immune response.Synonyms: CPAMD1, Complement component 3
Gene ID 24232
NCBI Accession NP_058690
UniProt P01026
Research Area Complement Factors
Pathways Complement System

Application Details

Product Details anti-C3 Antibody Target Details C3 Handling Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-C3 Antibody Target Details C3 Application Details Images back to top
Format Liquid
Reconstitution Restore by adding 1.0 mL of sterile distilled water
Concentration 10.0 mg/mL
Buffer PBS, pH 7.2 without preservatives and foreign proteins
Preservative Without preservative
Storage 4 °C/-20 °C
Storage Comment Store the antibody lyophilized at 2-8 °C and reconstituted at 2-8 °C for one week or (in aliquots) at -20 °C for longer. If a slight precipitation occurs upon storage, this should be removed by centrifugation.