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anti-Human CD55 antibody for Intracellular Flow Cytometry

Recommended CD55 Antibody (supplied by: Log in to see )

Antigen
Complement Decay-Accelerating Factor (CD55) Antibodies
  • AI323748
  • Cd55b
  • CR
  • CROM
  • Daf
  • DAF
  • Daf-GPI
  • Daf-TM
  • Daf1
  • GPI-DAF
  • TC
  • TM-DAF
Reactivity
Human
287
35
9
7
6
4
3
1
Host
Mouse
220
86
8
7
6
3
2
Clonality
Monoclonal
Conjugate
This CD55 antibody is conjugated to PE
49
44
25
10
4
3
3
3
2
2
1
1
1
1
Application
Intracellular Flow Cytometry (ICFC)
226
137
63
61
41
36
35
25
10
4
4
3
3
2
1
1
1
1
Supplier
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Catalog No. ABIN2663532
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008768327 ABIN2658691 FACS ICFC APC Mouse IgG1 kappa Log in to see JS11 3
0.0008768327 ABIN2664770 ICFC Mouse IgG1 kappa Log in to see JS11 3
0.0008768327 ABIN2658692 FACS ICFC APC Mouse IgG1 kappa Log in to see JS11 3
0.0008768327 ABIN2664771 ICFC Mouse IgG1 kappa Log in to see JS11 3

Top referenced anti-CD55 antibody for Intracellular Flow Cytometry

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General

Antigen Complement Decay-Accelerating Factor (CD55) Antibodies
Reactivity Human
(287), (35), (9), (7), (6), (4), (3), (1)
Host Mouse
(220), (86), (8), (7), (6), (3), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD55 antibody is conjugated to PE
(49), (44), (25), (10), (4), (3), (3), (3), (2), (2), (1), (1), (1), (1)
Application Intracellular Flow Cytometry (ICFC)
(226), (137), (63), (61), (41), (36), (35), (25), (10), (4), (4), (3), (3), (2), (1), (1), (1), (1)
Pubmed 4 references available
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Product Details anti-CD55 Antibody

Target Details CD55 Application Details Handling References for anti-CD55 Antibody (ABIN2663532) Images
Purification The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Clone JS11
Isotype IgG1 kappa

Target Details CD55

Product Details anti-CD55 Antibody Application Details Handling References for anti-CD55 Antibody (ABIN2663532) Images back to top
Antigen
Alternative Name CD55 (CD55 Antibody Abstract)
Background CD55 is a 60-70 kD glycosylphosphatidylinositol (GPI)-anchored single chain glycoprotein also known as decay-accelerating factor (DAF). It is expressed on hematopoietic cells including erythrocytes and many non-hematopoietic cells. CD55 accelerates the dissociation of the components of the C3-convertases (namely C2a from C4b in the C4bC2a complex, a C3-convertase of the classical pathway, and factor Bb from the C3bBb complex, a C3-convertase of the alternative pathway) to protect cells from inappropriate damage caused by autologous complement. CD55 has been reported to reduce the efficiency of NK cell lysis and induce signal transduction in T cells. CD55 has also been shown to interact with CD97 and bind to Coxsackie and Echovirus.
Research Area Immunology, CD Antigens, Surface Receptors of Immune Cells
Pathways Complement System

Application Details

Product Details anti-CD55 Antibody Target Details CD55 Handling References for anti-CD55 Antibody (ABIN2663532) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-CD55 Antibody Target Details CD55 Application Details References for anti-CD55 Antibody (ABIN2663532) Images back to top
Buffer Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide and 0.2 % (w/v) BSA .
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Protect from prolonged exposure to light. Do not freeze.
Storage 4 °C
Storage Comment The antibody solution should be stored undiluted between 2°C and 8°C.

References for anti-CD55 Antibody (ABIN2663532)

Product Details anti-CD55 Antibody Target Details CD55 Application Details Handling Images back to top
Background publications

DeZern, Uknis, Yuan, Mukhina, Varela, Saye, Pu, Brodsky: "Complement blockade with a C1 esterase inhibitor in paroxysmal nocturnal hemoglobinuria." in: Experimental hematology, Vol. 42, Issue 10, pp. 857-61.e1, 2014

Peyron, Bordier, NDiaye, Maridonneau-Parini: "Nonopsonic phagocytosis of Mycobacterium kansasii by human neutrophils depends on cholesterol and is mediated by CR3 associated with glycosylphosphatidylinositol-anchored proteins." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 165, Issue 9, pp. 5186-91, 2000

Hamann, Vogel, van Schijndel, van Lier: "The seven-span transmembrane receptor CD97 has a cellular ligand (CD55, DAF)." in: The Journal of experimental medicine, Vol. 184, Issue 3, pp. 1185-9, 1997

Fujita, Inoue, Ogawa, Iida, Tamura: "The mechanism of action of decay-accelerating factor (DAF). DAF inhibits the assembly of C3 convertases by dissociating C2a and Bb." in: The Journal of experimental medicine, Vol. 166, Issue 5, pp. 1221-8, 1987

Images

Product Details anti-CD55 Antibody Target Details CD55 Application Details Handling References for anti-CD55 Antibody (ABIN2663532) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-CD55 antibody (Complement Decay-Accelerating Factor)  (PE) (ABIN2663532) anti-Complement Decay-Accelerating Factor (CD55) antibody (PE)