anti-Human CD55 antibody for Immunofluorescence (fixed cells)

Recommended CD55 Antibody (supplied by: Log in to see )

Antigen
Complement Decay-Accelerating Factor (CD55) Antibodies
  • Daf
  • Daf-GPI
  • Daf1
  • GPI-DAF
  • CR
  • CROM
  • DAF
  • TC
  • AI323748
  • Cd55b
  • Daf-TM
  • TM-DAF
  • CD55 antigen
  • CD55 molecule, decay accelerating factor for complement (Cromer blood group)
  • decay accelerating factor 2
  • Cd55
  • CD55
  • Daf2
Epitope
AA 1-353
18
11
10
9
9
8
8
8
8
5
5
4
4
4
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Human
412
56
22
9
9
4
3
2
2
2
Host
Rabbit
339
96
24
8
7
3
3
Clonality
Monoclonal
Conjugate
This CD55 antibody is un-conjugated
53
47
30
13
11
11
10
9
8
8
6
6
6
6
6
6
5
5
5
4
4
4
3
3
2
2
1
1
1
Application
Cell Culture (CC), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF), Immunofluorescence (fixed cells) (IF/ICC)
359
186
97
63
56
54
42
39
12
8
6
6
5
5
2
1
1
1
1
1
1
Supplier
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Catalog No. ABIN1995655
$ 273.90
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN2989541 IF/ICC IHC WB Rabbit IgG Log in to see Polyclonal
1 ABIN3037432 FACS IF/ICC FITC Mouse IgG1 Log in to see
1 ABIN3037433 FACS IF/ICC PE Mouse IgG1 Log in to see
1 ABIN3067169 FACS IF/ICC Mouse IgG1 Log in to see

General

Antigen Complement Decay-Accelerating Factor (CD55) Antibodies
Epitope AA 1-353
(18), (11), (10), (9), (9), (8), (8), (8), (8), (5), (5), (4), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(412), (56), (22), (9), (9), (4), (3), (2), (2), (2)
Host Rabbit
(339), (96), (24), (8), (7), (3), (3)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD55 antibody is un-conjugated
(53), (47), (30), (13), (11), (11), (10), (9), (8), (8), (6), (6), (6), (6), (6), (6), (5), (5), (5), (4), (4), (4), (3), (3), (2), (2), (1), (1), (1)
Application Cell Culture (CC), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF), Immunofluorescence (fixed cells) (IF/ICC)
(359), (186), (97), (63), (56), (54), (42), (39), (12), (8), (6), (6), (5), (5), (2), (1), (1), (1), (1), (1), (1)
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Product Details anti-CD55 Antibody

Target Details CD55 Application Details Handling Images
Specificity Human CD55 / DAF
No cross-reactivity in ELISA with
Human CD97
No Cross-Reactivity Human
Characteristics This antibody was obtained from a rabbit immunized with purified, recombinant Human CD55 / DAF (rh CD55, ABIN2002066 NP_000565.1, Met 1-Ser 353,.
Sterility 0.2 μm filtered
Immunogen Recombinant Human CD55 protein (ABIN2002066)
Clone 028
Isotype IgG

Target Details CD55

Product Details anti-CD55 Antibody Application Details Handling Images back to top
Antigen
Alternative Name CD55 (CD55 Antibody Abstract)
Background CD55, also well known as decay-accelerating factor (DAF), is a member of the RCA (regulators of complement activation) family characterized by four to 30 SCRs (short consensus repeats) in their plasma-exposed regions. This 70 -75 kDa glycoprotein CD55 containing four SCR modules is involved in the regulation of the complement cascade. It interferes with the functions of C3 and C5 convertases in both the classical and alternative pathways by preventing the assembly of the C3bBb complex or accelerating the disassembly of preformed convertase, and thus disrupts the cascade and protects the host cells from damage.
NCBI Accession NP_000565.1
Research Area Immunology, CD Antigens, Surface Receptors of Immune Cells
Pathways Complement System, Regulation of Leukocyte Mediated Immunity

Application Details

Product Details anti-CD55 Antibody Target Details CD55 Handling Images back to top
Application Notes ELISA: 0.1-0.2 μg/mL
This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human CD55. The detection limit for Human CD55 is approximately 0.00975 ng/well.
ELISA(Cap): 0.5-4 μg/mL
ICC/IF: 10-25 μg/mL
FCM: 0.5-2 μg/Test
Restrictions For Research Use only

Handling

Product Details anti-CD55 Antibody Target Details CD55 Application Details Images back to top
Format Liquid
Buffer 0.2 μm filtered solution in PBS
Preservative Without preservative
Handling Advice Avoid repeated freeze-thaw cycles.
Storage 4 °C,-20 °C,-80 °C
Storage Comment This antibody can be stored at 2°C-8°C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20°C to -80°C. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Expiry Date 12 months

Images

Product Details anti-CD55 Antibody Target Details CD55 Application Details Handling back to top
Supplier Images
Immunofluorescence (IF) image for anti-Complement Decay-Accelerating Factor (CD55) (AA 1-353) antibody (ABIN1995655) Immunofluorescence staining of Human CD55 in JURKAT cells. Cells were fixed with 4% P...