You are viewing an incomplete version of our website. Please click to reload the website as full version.

anti-Mouse (Murine) CD55 antibody for Immunofluorescence

Recommended CD55 Antibody (supplied by: Log in to see )

Antigen
Complement Decay-Accelerating Factor (CD55) Antibodies
  • AI323748
  • Cd55b
  • CR
  • CROM
  • Daf
  • DAF
  • Daf-GPI
  • Daf-TM
  • Daf1
  • GPI-DAF
  • TC
  • TM-DAF
Reactivity
Mouse (Murine)
414
41
19
9
7
4
3
1
Host
Armenian Hamster
342
91
13
8
6
3
2
Clonality
Monoclonal
Conjugate
This CD55 antibody is conjugated to PE
54
50
30
10
10
8
8
8
8
7
6
6
6
6
6
6
6
5
5
4
3
3
2
2
2
2
1
1
1
Application
Flow Cytometry (FACS), Immunofluorescence (IF)
353
178
79
64
58
53
49
41
12
5
4
3
3
2
1
1
1
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Catalog No. ABIN1112474
$ 777.59
Plus shipping costs $45.00

Similar anti-CD55 Antibodies

Application / Reactivity Mouse (Murine)
ELISA 11 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies
Flow Cytometry (FACS) 32 Antibodies
Immunocytochemistry (ICC) 1 Antibodies
Immunofluorescence (IF) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 2 Antibodies
Western Blotting (WB) 15 Antibodies

General

Antigen Complement Decay-Accelerating Factor (CD55) Antibodies
Reactivity Mouse (Murine)
(414), (41), (19), (9), (7), (4), (3), (1)
Host Armenian Hamster
(342), (91), (13), (8), (6), (3), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD55 antibody is conjugated to PE
(54), (50), (30), (10), (10), (8), (8), (8), (8), (7), (6), (6), (6), (6), (6), (6), (6), (5), (5), (4), (3), (3), (2), (2), (2), (2), (1), (1), (1)
Application Flow Cytometry (FACS), Immunofluorescence (IF)
(353), (178), (79), (64), (58), (53), (49), (41), (12), (5), (4), (3), (3), (2), (1), (1), (1), (1)
Supplier Log in to see

Product Details anti-CD55 Antibody

Target Details CD55 Application Details Handling Images
Characteristics The anti-Mouse DAF monoclonal antibody detects DAF; one of several membrane proteins that prevent host cells from homologous complement attack. The two mouse DAF isoforms are effective against rat complement, but not against human and guinea pig complement. GPI-DAF is expressed only in testis. Both types of DAF transfectans avoided C3 deposition more successfully than non-transfectant cells.
Immunogen GPI-DAF transfected CHO cells. Species
Clone RIKO-3
Isotype IgG

Target Details CD55

Product Details anti-CD55 Antibody Application Details Handling Images back to top
Antigen
Alternative Name CD55 (CD55 Antibody Abstract)
Background CD55, also known as DAF (decay accelerating factor), is expressed on the surfaces of red blood cells, hematopoietic cells and many non-hemotopietic cells. There are two isoforms of mouse CD55, a GPI-anchored isoform and a transmembrane isoform. Both are recognized by RIKO-3. The function of CD55 is to protect damage by complement deposition
Research Area Immunology, CD Antigens, Surface Receptors of Immune Cells
Pathways Complement System

Application Details

Product Details anti-CD55 Antibody Target Details CD55 Handling Images back to top
Application Notes It is recommended for use in flow cytometry. This reagent is effective for direct immunofluorescence staining of mouse tissue for flow cytometric analysis using less than or equal to 0,25 µg/10^6 cells
Comment

R-Phycoerythrin (R-PE). Abs/Em: 545/575 nm.

Reagent Preparation 1. Transfer the sample of blood to a 12 x 75 mm polystyrene test tube (10^6 cells). 2. Add anti-mouse CD55 PE and mix gently with a vortex mixer. The optimal volume should be determined by the individual laboratory. CD55 recognizes the erythrocytes, so if you want a white blood cell labeling will be required prior lyse with ammonium chloride. 3. The recommended negative control is a non-reactive PE-conjugated antibody of the same isotype. 4. Incubate in the dark at room temperature at 4°C for 30 minutes or at room temperature (20-25 °C) for 15 minutes. 5. Add 1,5 mL of Lysing Solution to each sample and mix gently with a vortex mixer. Incubate for 10 minutes at room temperature in the dark. 6. Centrifuge at 1000 x g for 5 minutes. Gently aspirate the supernatant and discard it leaving approximately 50 µl of fluid. 7. Add 2 mL 0.01 mol/L PBS (It betters that it containing 2% bovine serum albumin) and resuspend the cells by using a vortex mixer. 8. Centrifuge at 1000 x g for 5 minutes. Gently aspirate the supernatant and discard it leaving approximately 50 µl of fluid. 9. Resuspend pellet in an appropriate fluid for flow cytometry, e.g. 0.3 mL PBS. The PBS should contain 1% paraformaldehyde (fixative) if samples are not analysed the same day. 10. Analyse on a flow cytometer or store at 2-8 °C in the dark until analysis. Samples can be run up to 24 hours after lysis
Restrictions For Research Use only

Handling

Product Details anti-CD55 Antibody Target Details CD55 Application Details Images back to top
Format Liquid
Buffer The conjugate is provided in liquid form in buffer containing 1% bovine serum albumin (BSA) and 0,09% Sodium azide, pH 7.2.
Preservative Sodium azide
Precaution of Use 1. The device is not intended for clinical use including diagnosis, prognosis, and monitoring of a disease state, and it must not be used in conjunction with patient records or treatment. 2. This product contains Sodium azide (NaN3), a chemical highly toxic in pure form. At product concentrations, though not classified as hazardous, Sodium azide may react with lead and copper plumbing to form highly explosive build-ups of metal azides. Upon disposal, flush with large volumes of water to prevent metal azide build-up in plumbing. 3. As with any product derived from biological sources, proper handling procedures should be used.
Storage 4 °C