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anti-Rat (Rattus) CD55 antibody for Functional Studies

Recommended CD55 Antibody (supplied by: Log in to see )

Antigen
Complement Decay-Accelerating Factor (CD55) Antibodies
  • AI323748
  • Cd55b
  • CR
  • CROM
  • Daf
  • DAF
  • Daf-GPI
  • Daf-TM
  • Daf1
  • GPI-DAF
  • TC
  • TM-DAF
Reactivity
Rat (Rattus)
414
42
19
9
6
4
3
1
Host
Mouse
341
91
13
8
7
3
2
Clonality
Monoclonal
Conjugate
This CD55 antibody is un-conjugated
54
51
30
10
10
8
8
8
8
7
6
6
6
6
6
6
6
5
5
4
3
3
2
2
2
2
1
1
1
Application
Functional Studies (Func), Western Blotting (WB)
354
177
79
64
58
54
49
41
12
5
4
3
2
2
1
1
1
1
Supplier
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Catalog No. ABIN2192123
$ 515.62
Plus shipping costs $45.00

Top referenced anti-CD55 antibody for Functional Studies

Similar anti-CD55 Antibodies

Application / Reactivity Rat (Rattus)
ELISA 4 Antibodies
Functional Studies (Func) 1 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 1 Antibodies
Immunohistochemistry (IHC) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 1 Antibodies
Western Blotting (WB) 6 Antibodies

General

Antigen Complement Decay-Accelerating Factor (CD55) Antibodies
Reactivity Rat (Rattus)
(414), (42), (19), (9), (6), (4), (3), (1)
Host Mouse
(341), (91), (13), (8), (7), (3), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD55 antibody is un-conjugated
(54), (51), (30), (10), (10), (8), (8), (8), (8), (7), (6), (6), (6), (6), (6), (6), (6), (5), (5), (4), (3), (3), (2), (2), (2), (2), (1), (1), (1)
Application Functional Studies (Func), Western Blotting (WB)
(354), (177), (79), (64), (58), (54), (49), (41), (12), (5), (4), (3), (2), (2), (1), (1), (1), (1)
Pubmed 5 references available
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Product Details anti-CD55 Antibody

Target Details CD55 Application Details Handling References for anti-CD55 Antibody (ABIN2192123) Images
Sterility 0.2 μm filtered
Clone RDIII-7

Target Details CD55

Product Details anti-CD55 Antibody Application Details Handling References for anti-CD55 Antibody (ABIN2192123) Images back to top
Antigen
Alternative Name Complement Decay Accelerating Factor (CD55 Antibody Abstract)
Background The monoclonal antibody RDIII-7 recognizes complement decay accelerating factor (DAF), also designated as CD55. RDIII-7 recognizes the common extracellular SCR region of CD55, detecting all isoforms of the protein. Cells express on their surface several proteins which protect against complement attack, namely C receptor I (CR1), decay accelerating factor (DAF), membrane cofactor protein (MCP) and CD59. CR1, DAF and MCP regulate the activation pathways of complement by either accelerating decay of the C3 and C5 convertase (CR1, DAF), or acting as cofactors for the serine protease factor I, which cleaves and irreversibly inactivates C3b (CR1, MCP). Rat DAF (CD55) is a 60 kDa transmembrane protein that binds C3b and C4b to inhibit formation and half- life of the C3 convertases. It belongs to the receptors of complement activation (RCA) family. DAF is broadly distributed among cells in contact with plasma complment proteins, including both haematopoietic and nonhaematopoietic cells. Although DAF does not have an essential role in controlling hemolysis of erythrocytes, it has an important role in regulation of the deposition of C3 on nucleated cells. Together with other complement regulators DAF protects self cells from autologous complement- mediated injury. DAF cooperates with CD46 in circumventing autologous C3 deposition, while CD59 inhibits the pathway at the critical end-point. Aliases DAF, CD55 Immunogen NIH-3T3 cells expressing GPI-anchored rat DAF
Pathways Complement System

Application Details

Product Details anti-CD55 Antibody Target Details CD55 Handling References for anti-CD55 Antibody (ABIN2192123) Images back to top
Application Notes For immunohistology, flow cytometry and Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For functional studies, in vitro dilutions have to be optimized in user's experimental setting.
Restrictions For Research Use only

Handling

Product Details anti-CD55 Antibody Target Details CD55 Application Details References for anti-CD55 Antibody (ABIN2192123) Images back to top
Buffer PBS, containing 0.1 % bovine serum albumin.
Storage 4 °C
Storage Comment Product should be stored at 4 °C Under recommended storage conditions, product is stable for one year.
Expiry Date 12 months

References for anti-CD55 Antibody (ABIN2192123)

Product Details anti-CD55 Antibody Target Details CD55 Application Details Handling Images back to top
Product cited in:

Mizuno, Harris, Morgan: "Spermatogenic cells distal to the blood-testis barrier in rats lack C3 convertase regulators and may be at risk of complement-mediated injury." in: Journal of reproductive immunology, Vol. 69, Issue 1, pp. 23-34, 2006

Mizuno, Donev, Harris, Morgan: "CD55 in rat male reproductive tissue: differential expression in testis and expression of a unique truncated isoform on spermatozoa." in: Molecular immunology, Vol. 44, Issue 7, pp. 1613-22, 2006

Harris, Williams, Linton, Morgan: "Coupling complement regulators to immunoglobulin domains generates effective anti-complement reagents with extended half-life in vivo." in: Clinical and experimental immunology, Vol. 129, Issue 2, pp. 198-207, 2002

Harris, Spiller, Morgan: "Human and rodent decay-accelerating factors (CD55) are not species restricted in their complement-inhibiting activities." in: Immunology, Vol. 100, Issue 4, pp. 462-70, 2000

Spiller, Harris, Morgan: "Efficient generation of monoclonal antibodies against surface-expressed proteins by hyperexpression in rodent cells." in: Journal of immunological methods, Vol. 224, Issue 1-2, pp. 51-60, 1999