anti-Rat (Rattus) CD55 antibody for Immunohistochemistry (Frozen Sections)

Recommended CD55 Antibody (supplied by: Log in to see )

Antigen
Complement Decay-Accelerating Factor (CD55) Antibodies
  • Daf
  • Daf-GPI
  • Daf1
  • GPI-DAF
  • CR
  • CROM
  • DAF
  • TC
  • AI323748
  • Cd55b
  • Daf-TM
  • TM-DAF
  • CD55 antigen
  • CD55 molecule, decay accelerating factor for complement (Cromer blood group)
  • decay accelerating factor 2
  • Cd55
  • CD55
  • Daf2
Reactivity
Rat (Rattus)
424
65
22
9
8
4
3
2
2
2
Host
Mouse
346
100
32
8
7
4
3
Clonality
Monoclonal
Conjugate
This CD55 antibody is un-conjugated
53
47
30
15
13
13
12
9
8
8
6
6
6
6
6
6
6
6
6
5
5
5
3
3
2
2
1
1
1
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
377
189
97
65
58
53
42
40
12
11
6
6
6
5
3
2
1
1
1
1
1
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Catalog No. ABIN2192124
$ 509.36
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN5541171 FACS Func ICC IF IHC (fro) WB Mouse IgG1 Log in to see RDIII-7
1 ABIN5541172 EIA FACS Func IHC (fro) WB Mouse IgG1 Log in to see RDIII-24

General

Antigen Complement Decay-Accelerating Factor (CD55) Antibodies
Reactivity Rat (Rattus)
(424), (65), (22), (9), (8), (4), (3), (2), (2), (2)
Host Mouse
(346), (100), (32), (8), (7), (4), (3)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD55 antibody is un-conjugated
(53), (47), (30), (15), (13), (13), (12), (9), (8), (8), (6), (6), (6), (6), (6), (6), (6), (6), (6), (5), (5), (5), (3), (3), (2), (2), (1), (1), (1)
Application Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
(377), (189), (97), (65), (58), (53), (42), (40), (12), (11), (6), (6), (6), (5), (3), (2), (1), (1), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-CD55 Antibody

Target Details CD55 Application Details Handling References for anti-CD55 antibody (ABIN2192124) Images
Sterility 0.2 μm filtered
Clone RDII-24

Target Details CD55

Product Details anti-CD55 Antibody Application Details Handling References for anti-CD55 antibody (ABIN2192124) Images back to top
Antigen
Alternative Name Complement Decay Accelerating Factor (CD55 Antibody Abstract)
Background The monoclonal antibody RDII-24 recognizes complement decay accelerating factor (DAF), also designated as CD55.. Cells express on their surface several proteins which protect against complement attack, namely C receptor I (CR1), decay accelerating factor (DAF), membrane cofactor protein (MCP) and CD59. CR1, DAF and MCP regulate the activation pathways of complement by either accelerating decay of the C3 and C5 convertase (CR1, DAF), or acting as cofactors for the serine protease factor I, which cleaves and irreversibly inactivates C3b (CR1, MCP). Rat DAF (CD55) is a 60 kDa transmembrane protein that binds C3b and C4b to inhibit formation and half- life of the C3 convertases. It belongs to the receptors of complement activation (RCA) family. DAF is broadly distributed among cells in contact with plasma complment proteins, including both haematopoietic and nonhaematopoietic cells. Although DAF does not have an essential role in controlling hemolysis of erythrocytes, it has an important role in regulation of the deposition of C3 on nucleated cells. Together with other complement regulators DAF protects self cells from autologous complement- mediated injury. DAF cooperates with CD46 in circumventing autologous C3 deposition, while CD59 inhibits the pathway at the critical end-point. Aliases DAF, CD55 Immunogen NIH-3T3 cells expressing GPI-anchored rat DAF
Pathways Complement System, Regulation of Leukocyte Mediated Immunity

Application Details

Product Details anti-CD55 Antibody Target Details CD55 Handling References for anti-CD55 antibody (ABIN2192124) Images back to top
Application Notes For immunohistology, flow cytometry and Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For functional studies, in vitro dilutions have to be optimized in user's experimental setting.
Restrictions For Research Use only

Handling

Product Details anti-CD55 Antibody Target Details CD55 Application Details References for anti-CD55 antibody (ABIN2192124) Images back to top
Buffer PBS, containing 0.1 % bovine serum albumin.
Storage 4 °C
Storage Comment Product should be stored at 4 °C Under recommended storage conditions, product is stable for one year.
Expiry Date 12 months

References for anti-CD55 antibody (ABIN2192124)

Product Details anti-CD55 Antibody Target Details CD55 Application Details Handling Images back to top
Product cited in:

Harris, Williams, Linton, Morgan: "Coupling complement regulators to immunoglobulin domains generates effective anti-complement reagents with extended half-life in vivo." in: Clinical and experimental immunology, Vol. 129, Issue 2, pp. 198-207, 2002

Spiller, Harris, Morgan: "Efficient generation of monoclonal antibodies against surface-expressed proteins by hyperexpression in rodent cells." in: Journal of immunological methods, Vol. 224, Issue 1-2, pp. 51-60, 1999