anti-Human CD59 antibody for Enzyme Immunoassay

Recommended CD59 Antibody (supplied by: Log in to see )

Antigen
CD59 Antibodies
  • 16.3A5
  • 1F5
  • EJ16
  • EJ30
  • EL32
  • G344
  • HRF-20
  • HRF20
  • MAC-IP
  • MACIF
  • MEM43
  • MIC11
  • MIN1
  • MIN2
  • MIN3
  • MIRL
  • MSK21
  • p18-20
  • Cd59a
  • Cd59b
  • MACIP
  • AA987121
  • Cd59
  • protectin
  • CD59 molecule, complement regulatory protein
  • CD59a antigen
  • CD59b antigen
  • CD59
  • Cd59
  • Cd59a
  • Cd59b
Reactivity
Human
510
43
38
23
17
14
9
8
2
2
1
1
Host
Mouse
408
125
41
7
1
Clonality
Monoclonal
Conjugate
This CD59 antibody is un-conjugated
63
53
44
14
12
12
12
11
11
10
10
10
10
10
10
10
6
6
5
1
1
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
423
207
192
130
113
90
82
46
45
25
13
9
8
3
3
2
1
1
1
1
1
1
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Catalog No. ABIN118747
$ 1,061.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN2718236 EIA WB Rabbit IgG Log in to see Polyclonal

General

Antigen CD59 Antibodies
Reactivity Human
(510), (43), (38), (23), (17), (14), (9), (8), (2), (2), (1), (1)
Host Mouse
(408), (125), (41), (7), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD59 antibody is un-conjugated
(63), (53), (44), (14), (12), (12), (12), (11), (11), (10), (10), (10), (10), (10), (10), (10), (6), (6), (5), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
(423), (207), (192), (130), (113), (90), (82), (46), (45), (25), (13), (9), (8), (3), (3), (2), (1), (1), (1), (1), (1), (1)
Pubmed 4 references available
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Product Details anti-CD59 Antibody

Target Details CD59 Application Details Handling References for anti-CD59 antibody (ABIN118747) Images
Specificity This antibody reacts with a PI-linked glycoprotein, Mw 18-20 kDa found on all types of leucocytes including platelets. MEM-43 has been shown to stimulate NK activity.
Purification Affinity chromatography on Protein A
Clone MEM-43
Isotype IgG2a

Target Details CD59

Product Details anti-CD59 Antibody Application Details Handling References for anti-CD59 antibody (ABIN118747) Images back to top
Antigen
Alternative Name CD59 (CD59 Antibody Abstract)
Background The antigen is expressed on many haemapoietic and non-haemapoietic cells.Synonyms: 20 kDa homologous restriction factor, HRF-20, HRF20, MAC-IP, MAC-inhibitory protein, MACIF, MEM43 antigen, MIC11, MIN1, MIN2, MIN3, MIRL, MSK21, Membrane attack complex inhibition factor, Membrane inhibitor of reactive lysis, Protectin
Gene ID 966
NCBI Accession NP_000602
UniProt P13987
Research Area Cardiovascular, Atherosclerosis, Hematopoietic Progenitors, Tyrosine Kinases, Innate Immunity, CD Antigens, Surface Receptors of Immune Cells
Pathways Complement System

Application Details

Product Details anti-CD59 Antibody Target Details CD59 Handling References for anti-CD59 antibody (ABIN118747) Images back to top
Application Notes Flow Cytometry: 1/5 - 1/10, Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. ELISA. Immunoprecipitation. Western Blot: The epitope recognised by MEM-43 is lost after reduction, therefore, non- reducing conditions are required for western blotting techniques. Immunohistochemistry on frozen and paraffin sections: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections, Sodium citrate buffer pH 6.0 is recommended for this purpose. Recommended Positive Control: Tonsil.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-CD59 Antibody Target Details CD59 Application Details References for anti-CD59 antibody (ABIN118747) Images back to top
Concentration 1.0 mg/mL
Buffer PBS
Preservative Azide free
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store the antibody at -20 °C. Can be shipped at 2 - 8 °C.

References for anti-CD59 antibody (ABIN118747)

Product Details anti-CD59 Antibody Target Details CD59 Application Details Handling Images back to top
Background publications

Tandon, Yan, Morgan, Weetman: "Expression and function of multiple regulators of complement activation in autoimmune thyroid disease." in: Immunology, Vol. 81, Issue 4, pp. 643-7, 1994

Stefanová, Horejsí: "Association of the CD59 and CD55 cell surface glycoproteins with other membrane molecules." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 147, Issue 5, pp. 1587-92, 1991

Stefanová, Hilgert, Kristofová, Brown, Low, Horejsí: "Characterization of a broadly expressed human leucocyte surface antigen MEM-43 anchored in membrane through phosphatidylinositol." in: Molecular immunology, Vol. 26, Issue 2, pp. 153-61, 1989

Horejsí, Angelisová, Bazil, Kristofová, Stoyanov, Stefanová, Hausner, Vosecký, Hilgert et al.: "Monoclonal antibodies against human leucocyte antigens. II. Antibodies against CD45 (T200), CD3 (T3), CD43, CD10 (CALLA), transferrin receptor (T9), a novel broadly expressed 18-kDa antigen (MEM-43) ..." in: Folia biologica, Vol. 34, Issue 1, pp. 23-34, 1988

Images

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Supplier Images
Flow Cytometry (FACS) image for anti-CD59 (CD59) antibody (ABIN118747) Staining of human peripheral blood lymphocytes with MOUSE ANTI HUMAN CD59: AZIDE FREE...