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anti-Human CD59 antibody for Flow Cytometry

Recommended CD59 Antibody (supplied by: Log in to see )

Antigen
CD59 Antibodies
  • 1F5
  • 16.3A5
  • AA987121
  • Cd59
  • Cd59a
  • Cd59b
  • EJ16
  • EJ30
  • EL32
  • G344
  • HRF-20
  • HRF20
  • MAC-IP
  • MACIF
  • MACIP
  • MEM43
  • MIC11
  • MIN1
  • MIN2
  • MIN3
  • MIRL
  • MSK21
  • p18-20
  • protectin
Reactivity
Human
340
22
19
10
9
7
2
2
2
1
1
Host
Rat
249
109
24
7
1
Clonality
Monoclonal
Conjugate
This CD59 antibody is un-conjugated
57
48
38
10
9
2
2
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
244
137
96
78
76
69
31
28
17
14
11
6
5
3
2
2
1
Supplier
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Catalog No. ABIN119472
$ 467.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.30190992 ABIN118747 EIA FACS IHC (fro) IHC (p) IP WB Mouse IgG2a Log in to see MEM-43 4
0.30190992 ABIN1449270 FACS FITC Mouse IgG2b Log in to see WK8 2
0.30190992 ABIN1449269 FACS IP Mouse IgG2b Log in to see WK8 2
0.30190992 ABIN118753 FACS PE Mouse IgG2a Log in to see MEM-43 4
0.30190992 ABIN1098134 ICC FACS IHC ELISA WB Mouse IgG1 AA 31-111 Log in to see 8D2B8 2
0.30190992 ABIN94200 FACS Biotin Mouse IgG2a Log in to see MEM-43 9
0.30190992 ABIN951359 FACS Biotin Mouse IgG1 Log in to see 1F5 6
0.30190992 ABIN951360 FACS FITC Mouse IgG1 Log in to see 1F5 6
0.30190992 ABIN951361 FACS PE Mouse IgG1 Log in to see 1F5 6
0.30190992 ABIN94199 FACS IHC (p) IP Mouse IgG2a Log in to see MEM-43 9
0.30190992 ABIN94201 FACS FITC Mouse IgG2a Log in to see MEM-43 9
0.30190992 ABIN125700 FACS APC Mouse IgG2a Log in to see MEM-43 8
0.30190992 ABIN125699 FACS IHC (p) IP PE Mouse IgG2a Log in to see MEM-43 9
0.30190992 ABIN2663538 FACS PE Mouse IgG2a kappa Log in to see p282 (H19) 3
0.30190992 ABIN2661788 FACS FITC Mouse IgG2a kappa Log in to see p282 (H19) 4
0.30190992 ABIN2145201 FACS Mouse IgG1 Log in to see 4AOV9A2 2
0.30190992 ABIN2145203 FACS Mouse IgG1 Log in to see 4AOV9A2 2
0.30190992 ABIN2664779 FACS Mouse IgG2a kappa Log in to see p282 (H19) 3
0.30190992 ABIN262738 FACS FITC Mouse IgG2a kappa Log in to see p282
0.30190992 ABIN262736 FACS Mouse IgG2a kappa Log in to see p282

Top referenced anti-CD59 antibody for Flow Cytometry

Similar anti-CD59 Antibodies

Application / Reactivity Human
Blocking Antibody (Inhibition) 1 Antibodies
ELISA 68 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies
Flow Cytometry (FACS) 241 Antibodies
Functional Studies (Func) 18 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 8 Antibodies
Immunofluorescence (IF) 32 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 75 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 6 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 5 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 23 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 67 Antibodies
Immunoprecipitation (IP) 68 Antibodies

General

Antigen CD59 Antibodies
Reactivity Human
(340), (22), (19), (10), (9), (7), (2), (2), (2), (1), (1)
Host Rat
(249), (109), (24), (7), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD59 antibody is un-conjugated
(57), (48), (38), (10), (9), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(244), (137), (96), (78), (76), (69), (31), (28), (17), (14), (11), (6), (5), (3), (2), (2), (1)
Pubmed 6 references available
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Product Details anti-CD59 Antibody

Target Details CD59 Application Details Handling References for anti-CD59 Antibody (ABIN119472) Images
Specificity This antibody recognises the CD59 cell surface antigen. Clone YTH53.1 has been shown to block the normal function of CD59.
Purification Affinity Chromatography on Protein G
Immunogen Human Peripheral Blood T cells. Spleen cells from an immunised DA rat were fused with cells of the Y3/Ag1.2.3 rat myeloma line.
Clone YTH53-1
Isotype IgG2b

Target Details CD59

Product Details anti-CD59 Antibody Application Details Handling References for anti-CD59 Antibody (ABIN119472) Images back to top
Antigen
Alternative Name CD59 (CD59 Antibody Abstract)
Background CD59 is an 18-20kD GPI linked glycoprotein broadly expressed by human leucocytes and erythrocytes. CD59 is expressed by erythrocytes, and therefore staining in whole blood systems requires a higher concentration of antibody.Synonyms: 20 kDa homologous restriction factor, HRF-20, HRF20, MAC-IP, MAC-inhibitory protein, MACIF, MEM43 antigen, MIC11, MIN1, MIN2, MIN3, MIRL, MSK21, Membrane attack complex inhibition factor, Membrane inhibitor of reactive lysis, Protectin
Gene ID 966
NCBI Accession NP_000602
UniProt P13987
Research Area Cardiovascular, Atherosclerosis, Hematopoietic Progenitors, Tyrosine Kinases, Innate Immunity, CD Antigens, Surface Receptors of Immune Cells
Pathways Complement System

Application Details

Product Details anti-CD59 Antibody Target Details CD59 Handling References for anti-CD59 Antibody (ABIN119472) Images back to top
Application Notes Western Blot. Flow cytometry: 1/50 - 1/100, Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Immunohistochemistry on frozen sections. Immunohistochemistry on paraffin sections: 1/5 - 1/10, This product requires antigen retrieval using heat treatment prior to staining of paraffin sections, Sodium citrate buffer pH 6.0 is recommended for this purpose. Recommended Positive Control: Placenta. Removal of sodium azide is recommended prior to use in functional assays.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-CD59 Antibody Target Details CD59 Application Details References for anti-CD59 Antibody (ABIN119472) Images back to top
Concentration 1.0 mg/mL
Buffer PBS containing 0.09 % Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-CD59 Antibody (ABIN119472)

Product Details anti-CD59 Antibody Target Details CD59 Application Details Handling Images back to top
Background publications

Yang, Tyrrell, Han, Jaffe: "Expression and modulation of RPE cell membrane complement regulatory proteins." in: Investigative ophthalmology & visual science, Vol. 50, Issue 7, pp. 3473-81, 2009

Ajona, Hsu, Corrales, Montuenga, Pio: "Down-regulation of human complement factor H sensitizes non-small cell lung cancer cells to complement attack and reduces in vivo tumor growth." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 178, Issue 9, pp. 5991-8, 2007

Davies, Wilson, Bramley, Willers, Van Heyningen, Bickmore, Lachmann: "Identification of MIC 11 antigen as an epitope of the CD59 molecule." in: Immunology, Vol. 85, Issue 2, pp. 220-7, 1995

Walsh, Tone, Waldmann: "Transfection of human CD59 complementary DNA into rat cells confers resistance to human complement." in: European journal of immunology, Vol. 21, Issue 3, pp. 847-50, 1991

Davies, Simmons, Hale, Harrison, Tighe, Lachmann, Waldmann: "CD59, an LY-6-like protein expressed in human lymphoid cells, regulates the action of the complement membrane attack complex on homologous cells." in: The Journal of experimental medicine, Vol. 170, Issue 3, pp. 637-54, 1989

Gilman, Wiggs, Chamberlin: "Nucleotide sequences of two Bacillus subtilis promoters used by Bacillus subtilis sigma-28 RNA polymerase." in: Nucleic acids research, Vol. 9, Issue 22, pp. 5991-6000, 1982

Images

Product Details anti-CD59 Antibody Target Details CD59 Application Details Handling References for anti-CD59 Antibody (ABIN119472) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-CD59 antibody (CD59) (ABIN119472) Staining of human peripheral blood granulocytes with RAT ANTI HUMAN CD59 (ABIN119472).