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anti-Human CD59 antibody for Functional Studies

Recommended CD59 Antibody (supplied by: Log in to see )

Antigen
CD59 Antibodies
  • 16.3A5
  • 1F5
  • EJ16
  • EJ30
  • EL32
  • G344
  • HRF-20
  • HRF20
  • MAC-IP
  • MACIF
  • MEM43
  • MIC11
  • MIN1
  • MIN2
  • MIN3
  • MIRL
  • MSK21
  • p18-20
  • Cd59a
  • Cd59b
  • MACIP
  • AA987121
  • Cd59
  • protectin
  • CD59 molecule, complement regulatory protein
  • CD59a antigen
  • CD59b antigen
  • CD59
  • Cd59
  • Cd59a
  • Cd59b
Reactivity
Human
473
42
36
23
17
14
9
8
2
2
1
1
Host
Mouse
371
123
40
7
1
Clonality
Monoclonal
Conjugate
This CD59 antibody is un-conjugated
58
51
42
13
11
11
10
10
10
9
9
9
9
9
9
9
5
5
5
1
1
1
1
1
1
1
1
1
1
Application
Functional Studies (Func), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
387
189
184
116
97
82
69
42
30
19
13
9
8
3
2
2
1
1
1
1
1
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Catalog No. ABIN118751
$ 467.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN120694 Func FACS Mouse IgM Log in to see MEM-129 1
1 ABIN94204 Func FACS Mouse IgM Log in to see MEM-129 6
1 ABIN260113 Func ICC FACS IHC (fro) IF IHC (p) IHC WB Rat IgG2b Log in to see YTH53-1
1 ABIN3025490 Func FACS IF IHC (p) Mouse IgG1, kappa Log in to see MACIF-629
1 ABIN206909 Func FACS Mouse IgM Log in to see MEM-129
1 ABIN3024439 Func FACS IF IHC (p) Mouse IgG1, kappa Log in to see SPM616
1 ABIN2481021 Func IHC (fro) FACS IF IHC (p) WB Rat IgG2b Log in to see YTH53-1 4
1 ABIN2481022 Func IHC (fro) FACS IHC (p) WB Rat IgG2b Log in to see YTH53-1 4
1 ABIN269160 Func FACS Mouse IgM Log in to see MEM-129
1 ABIN4294909 FACS Func PerCP Mouse IgM Log in to see MEM-129
1 ABIN3025483 Func FACS IF Mouse IgM, kappa Log in to see 193-27
1 ABIN3199956 FACS Func Mouse IgM Log in to see MEM-129
1 ABIN3025488 Func FACS IF Mouse IgG1, kappa Log in to see BRA-10G
1 ABIN3025484 Func FACS IF Mouse IgM, kappa Log in to see 193-27
1 ABIN3025489 Func FACS IF Mouse IgG1, kappa Log in to see BRA-10G
1 ABIN3025492 Func FACS IF IHC (p) Mouse IgG1, kappa Log in to see MACIF-629
1 ABIN3024440 Func FACS IF IHC (p) Mouse IgG1, kappa Log in to see SPM616
1 ABIN3037467 Func FACS Mouse IgM Log in to see

Top referenced anti-CD59 antibody for Functional Studies

Similar anti-CD59 Antibodies

Application / Reactivity Human
Blocking Antibody (Inhibition) 1 Antibodies
Blocking Peptide (BP) 1 Antibodies
Cell Culture (CC) 1 Antibodies
Cytometry by Time of Flight (CyTOF) 9 Antibodies
ELISA 62 Antibodies
ELISA (Detection) 1 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies
Flow Cytometry (FACS) 358 Antibodies
Functional Studies (Func) 19 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 23 Antibodies
Immunofluorescence (IF) 69 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 3 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 13 Antibodies
Immunohistochemistry (IHC) 100 Antibodies

General

Antigen CD59 Antibodies
Reactivity Human
(473), (42), (36), (23), (17), (14), (9), (8), (2), (2), (1), (1)
Host Mouse
(371), (123), (40), (7), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD59 antibody is un-conjugated
(58), (51), (42), (13), (11), (11), (10), (10), (10), (9), (9), (9), (9), (9), (9), (9), (5), (5), (5), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Functional Studies (Func), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(387), (189), (184), (116), (97), (82), (69), (42), (30), (19), (13), (9), (8), (3), (2), (2), (1), (1), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-CD59 Antibody

Target Details CD59 Application Details Handling References for anti-CD59 Antibody (ABIN118751) Images
Specificity Reacts with a PI-linked glycoprotein, Mw 18-20 kDa found on all types of leucocytes including platelets. The antigen is expressed on many haemapoietic and non-haemapoietic cells. MEM-43 has been shown to stimulate NK activity. CD59 is expressed by erythrocytes and therefore staining in whole blood systems requires a high concentration of antibody. The epitope recognised by MEM-43 is lost after reduction, therefore, non-reducing conditions are required for western blotting techniques (2, 5).
Purification Affinity Chromatography on Protein A.
Clone MEM-43
Isotype IgG2a

Target Details CD59

Product Details anti-CD59 Antibody Application Details Handling References for anti-CD59 Antibody (ABIN118751) Images back to top
Antigen
Alternative Name CD59 (CD59 Antibody Abstract)
Background CD59, an LY6 like protein expressed in human lymphoid cells, regulates the action of the complement membrane attack complex on homologous cells. It is a potent inhibitor of the complement membrane attack complex action. It acts by binding to the C8 and/or C9 complements of the membrane attack complex, thereby preventing incorporation of the multiple copies of C9 required for complete formation of the osmolytic pore. This inhibitor appears to be species-specific. CD59 is also involved in signal transduction for T-cell activation complexed to a protein tyrosine kinase.Synonyms: 20 kDa homologous restriction factor, HRF-20, HRF20, MAC-IP, MAC-inhibitory protein, MACIF, MEM43 antigen, MIC11, MIN1, MIN2, MIN3, MIRL, MSK21, Membrane attack complex inhibition factor, Membrane inhibitor of reactive lysis, Protectin
Gene ID 966
NCBI Accession NP_000602
UniProt P13987
Research Area Cardiovascular, Atherosclerosis, Hematopoietic Progenitors, Tyrosine Kinases, Innate Immunity, CD Antigens, Surface Receptors of Immune Cells
Pathways Complement System

Application Details

Product Details anti-CD59 Antibody Target Details CD59 Handling References for anti-CD59 Antibody (ABIN118751) Images back to top
Application Notes ELISA. Immunoprecipitation. Western blot (non-reducing conditions). Flow Cytometry (Use 10 μL of 1/5-1/10 diluted antibody to label 106 cells in 100 μL). Immunohistochemistry on Frozen and Paraffin Sections: Requires antigen retrieval using heat treatment methods prior to staining of paraffin sections, Sodium citrate buffer pH 6.0 is recommended for this purpose. Positive control: Tonsil.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-CD59 Antibody Target Details CD59 Application Details References for anti-CD59 Antibody (ABIN118751) Images back to top
Concentration 1.0 mg/mL
Buffer PBS with 0.09 % Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-CD59 Antibody (ABIN118751)

Product Details anti-CD59 Antibody Target Details CD59 Application Details Handling Images back to top
Background publications

Stefanová, Horejsí: "Association of the CD59 and CD55 cell surface glycoproteins with other membrane molecules." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 147, Issue 5, pp. 1587-92, 1991

Horejsí, Angelisová, Bazil, Kristofová, Stoyanov, Stefanová, Hausner, Vosecký, Hilgert et al.: "Monoclonal antibodies against human leucocyte antigens. II. Antibodies against CD45 (T200), CD3 (T3), CD43, CD10 (CALLA), transferrin receptor (T9), a novel broadly expressed 18-kDa antigen (MEM-43) ..." in: Folia biologica, Vol. 34, Issue 1, pp. 23-34, 1988