anti-Human CD59 antibody for Immunohistochemistry (Frozen Sections)

Recommended CD59 Antibody (supplied by: Log in to see )

Antigen
CD59 Antibodies
  • 16.3A5
  • 1F5
  • EJ16
  • EJ30
  • EL32
  • G344
  • HRF-20
  • HRF20
  • MAC-IP
  • MACIF
  • MEM43
  • MIC11
  • MIN1
  • MIN2
  • MIN3
  • MIRL
  • MSK21
  • p18-20
  • Cd59a
  • Cd59b
  • MACIP
  • AA987121
  • Cd59
  • protectin
  • CD59 molecule, complement regulatory protein
  • CD59a antigen
  • CD59b antigen
  • CD59
  • Cd59
  • Cd59a
  • Cd59b
Reactivity
Human
509
43
38
23
17
14
9
8
2
2
1
1
Host
Rat
409
124
40
7
1
Clonality
Monoclonal
Conjugate
This CD59 antibody is un-conjugated
63
53
44
14
12
12
12
11
11
10
10
10
10
10
10
10
6
6
5
1
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
423
206
192
130
112
90
83
46
45
25
13
9
8
4
3
2
1
1
1
1
1
1
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Catalog No. ABIN119472
$ 467.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN118747 EIA FACS IHC (fro) IHC (p) IP WB Mouse IgG2a Log in to see MEM-43 4
1 ABIN118751 Func FACS IHC (fro) IHC (p) WB Mouse IgG2a Log in to see MEM-43 2
1 ABIN1106481 IHC (fro) FACS IF FITC Mouse IgG1 Log in to see NaM172-2B5 6
1 ABIN1106482 IHC (fro) FACS IF PE Mouse IgG1 Log in to see NaM172-2B5 6
1 ABIN320004 FACS IHC (fro) WB Rat IgG2b Log in to see YTH53-1 4
1 ABIN2481003 ELISA FACS IEM IF IHC (fro) IHC (p) IP WB Mouse IgG2a Log in to see MEM-43 1
1 ABIN2481005 IHC (fro) FACS WB Mouse IgG2b Log in to see MEM-43-5 3
1 ABIN260113 Func ICC FACS IHC (fro) IF IHC (p) IHC WB Rat IgG2b Log in to see YTH53-1
1 ABIN625888 IHC (fro) FACS IHC (p) WB Rat IgG2b Log in to see
1 ABIN2481021 Func IHC (fro) FACS IF IHC (p) WB Rat IgG2b Log in to see YTH53-1 4
1 ABIN2481022 Func IHC (fro) FACS IHC (p) WB Rat IgG2b Log in to see YTH53-1 4
1 ABIN298471 FACS IHC (fro) IHC (p) IP ELISA WB Mouse IgG2a Log in to see MEM-43
1 ABIN211869 FACS IHC (fro) IHC (p) IP ELISA WB Mouse IgG2a Log in to see MEM-43
1 ABIN609364 IHC (fro) FACS IHC (p) WB Rat IgG2b Log in to see
1 ABIN576561 FACS IHC (fro) IHC (p) WB PE Rat IgG2b Log in to see
1 ABIN217791 FACS IHC (fro) IHC (p) WB FITC Rat IgG2b Log in to see
1 ABIN4295048 FACS ICC IF IHC (fro) IHC (p) WB Alexa Fluor 700 Rat IgG2b Log in to see YTH53-1
1 ABIN4295045 FACS ICC IF IHC (fro) IHC (p) WB Alexa Fluor 405 Rat IgG2b Log in to see YTH53-1
1 ABIN4295050 ICC IF IHC (fro) IHC (p) WB Biotin Rat IgG2b Log in to see YTH53-1
1 ABIN4295053 FACS ICC IF IHC (fro) IHC (p) WB DyLight 680 Rat IgG2b Log in to see YTH53-1

General

Antigen CD59 Antibodies
Reactivity Human
(509), (43), (38), (23), (17), (14), (9), (8), (2), (2), (1), (1)
Host Rat
(409), (124), (40), (7), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD59 antibody is un-conjugated
(63), (53), (44), (14), (12), (12), (12), (11), (11), (10), (10), (10), (10), (10), (10), (10), (6), (6), (5), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(423), (206), (192), (130), (112), (90), (83), (46), (45), (25), (13), (9), (8), (4), (3), (2), (1), (1), (1), (1), (1), (1)
Pubmed 6 references available
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Product Details anti-CD59 Antibody

Target Details CD59 Application Details Handling ProductDetails: References for anti-CD59 antibody (ABIN119472) Images
Specificity This antibody recognises the CD59 cell surface antigen. Clone YTH53.1 has been shown to block the normal function of CD59.
Purification Affinity Chromatography on Protein G
Immunogen Human Peripheral Blood T cells. Spleen cells from an immunised DA rat were fused with cells of the Y3/Ag1.2.3 rat myeloma line.
Clone YTH53-1
Isotype IgG2b

Target Details CD59

Product Details anti-CD59 Antibody Application Details Handling ProductDetails: References for anti-CD59 antibody (ABIN119472) Images back to top
Antigen
Alternative Name CD59 (CD59 Antibody Abstract)
Background CD59 is an 18-20kD GPI linked glycoprotein broadly expressed by human leucocytes and erythrocytes. CD59 is expressed by erythrocytes, and therefore staining in whole blood systems requires a higher concentration of antibody.Synonyms: 20 kDa homologous restriction factor, HRF-20, HRF20, MAC-IP, MAC-inhibitory protein, MACIF, MEM43 antigen, MIC11, MIN1, MIN2, MIN3, MIRL, MSK21, Membrane attack complex inhibition factor, Membrane inhibitor of reactive lysis, Protectin
Gene ID 966
NCBI Accession NP_000602
UniProt P13987
Research Area Cardiovascular, Atherosclerosis, Hematopoietic Progenitors, Tyrosine Kinases, Innate Immunity, CD Antigens, Surface Receptors of Immune Cells
Pathways Complement System

Application Details

Product Details anti-CD59 Antibody Target Details CD59 Handling ProductDetails: References for anti-CD59 antibody (ABIN119472) Images back to top
Application Notes Western Blot. Flow cytometry: 1/50 - 1/100, Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Immunohistochemistry on frozen sections. Immunohistochemistry on paraffin sections: 1/5 - 1/10, This product requires antigen retrieval using heat treatment prior to staining of paraffin sections, Sodium citrate buffer pH 6.0 is recommended for this purpose. Recommended Positive Control: Placenta. Removal of sodium azide is recommended prior to use in functional assays.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-CD59 Antibody Target Details CD59 Application Details ProductDetails: References for anti-CD59 antibody (ABIN119472) Images back to top
Concentration 1.0 mg/mL
Buffer PBS containing 0.09 % Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

ProductDetails: References for anti-CD59 antibody (ABIN119472)

Product Details anti-CD59 Antibody Target Details CD59 Application Details Handling Images back to top
Background publications

Yang, Tyrrell, Han, Jaffe: "Expression and modulation of RPE cell membrane complement regulatory proteins." in: Investigative ophthalmology & visual science, Vol. 50, Issue 7, pp. 3473-81, 2009

Ajona, Hsu, Corrales, Montuenga, Pio: "Down-regulation of human complement factor H sensitizes non-small cell lung cancer cells to complement attack and reduces in vivo tumor growth." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 178, Issue 9, pp. 5991-8, 2007

Davies, Wilson, Bramley, Willers, Van Heyningen, Bickmore, Lachmann: "Identification of MIC 11 antigen as an epitope of the CD59 molecule." in: Immunology, Vol. 85, Issue 2, pp. 220-7, 1995

Walsh, Tone, Waldmann: "Transfection of human CD59 complementary DNA into rat cells confers resistance to human complement." in: European journal of immunology, Vol. 21, Issue 3, pp. 847-50, 1991

Davies, Simmons, Hale, Harrison, Tighe, Lachmann, Waldmann: "CD59, an LY-6-like protein expressed in human lymphoid cells, regulates the action of the complement membrane attack complex on homologous cells." in: The Journal of experimental medicine, Vol. 170, Issue 3, pp. 637-54, 1989

Gilman, Wiggs, Chamberlin: "Nucleotide sequences of two Bacillus subtilis promoters used by Bacillus subtilis sigma-28 RNA polymerase." in: Nucleic acids research, Vol. 9, Issue 22, pp. 5991-6000, 1982

Images

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Supplier Images
Flow Cytometry (FACS) image for anti-CD59 (CD59) antibody (ABIN119472) Staining of human peripheral blood granulocytes with RAT ANTI HUMAN CD59 (ABIN119472).