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anti-Human CD59 antibody for Immunoprecipitation

Recommended CD59 Antibody (supplied by: Log in to see )

Antigen
CD59 Antibodies
  • 1F5
  • 16.3A5
  • AA987121
  • Cd59
  • Cd59a
  • Cd59b
  • EJ16
  • EJ30
  • EL32
  • G344
  • HRF-20
  • HRF20
  • MAC-IP
  • MACIF
  • MACIP
  • MEM43
  • MIC11
  • MIN1
  • MIN2
  • MIN3
  • MIRL
  • MSK21
  • p18-20
  • protectin
Epitope
AA 1-101
13
7
7
7
7
6
5
5
5
4
3
2
2
1
1
1
Reactivity
Human
340
22
19
10
9
7
2
2
2
1
1
Host
Rabbit
249
108
25
7
1
Clonality
Polyclonal
Conjugate
This CD59 antibody is un-conjugated
57
48
38
10
9
2
2
1
1
1
1
1
1
1
1
1
Minimum Detection Limit
0.00975 ng/well
Application
Immunoprecipitation (IP), ELISA, Western Blotting (WB)
245
137
95
78
75
70
31
29
17
14
11
6
5
3
2
2
1
Supplier
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Catalog No. ABIN1999739
$ 218.90
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.00089235755 ABIN118747 EIA FACS IHC (fro) IHC (p) IP WB Mouse IgG2a Log in to see MEM-43 4
0.00089235755 ABIN1449269 FACS IP Mouse IgG2b Log in to see WK8 2
0.00089235755 ABIN118752 FACS IHC (p) IP Mouse IgG2a Log in to see MEM-43 12
0.00089235755 ABIN400666 FACS IHC (p) IP WB Mouse IgG2b Log in to see MEM-43-5
0.00089235755 ABIN94199 FACS IHC (p) IP Mouse IgG2a Log in to see MEM-43 9
0.00089235755 ABIN94203 FACS IHC (p) IP WB Mouse IgG2b Log in to see MEM-43-5 7
0.00089235755 ABIN125699 FACS IHC (p) IP PE Mouse IgG2a Log in to see MEM-43 9
0.00089235755 ABIN298470 FACS IHC (p) IP WB Mouse IgG2b Log in to see MEM-43-5
0.00089235755 ABIN290285 FACS IHC (p) IP Mouse IgG2a Log in to see MEM-43
0.00089235755 ABIN234709 FACS IP Mouse IgG2a Log in to see MEM-43 1
0.00089235755 ABIN120611 FACS IHC (p) IP WB Mouse IgG2b Log in to see MEM-43-5
0.00089235755 ABIN2469884 FACS IP IHC Mouse IgG2a Log in to see MEM-43
0.00089235755 ABIN2469885 FACS IP IHC WB Mouse IgG2b Log in to see MEM-43-5
0.00089235755 ABIN298471 FACS IHC (fro) IHC (p) IP ELISA WB Mouse IgG2a Log in to see MEM-43
0.00089235755 ABIN290056 FACS IP Mouse IgG1 kappa Log in to see YUK1
0.00089235755 ABIN1723362 FACS IHC (p) IP WB Mouse IgG2b Log in to see MEM-43-5
0.00089235755 ABIN1723361 FACS IHC (p) IP Mouse IgG2a Log in to see MEM-43
0.00089235755 ABIN2165016 IHC (af) IHC (fp) FACS IP ELISA WB Mouse IgG2a Log in to see
0.00089235755 ABIN2481003 IHC (fro) FACS IF IHC (p) IP ELISA WB Mouse IgG2a Log in to see MEM-43 1
0.00089235755 ABIN2470177 FACS IP IHC ELISA WB Mouse IgG2a Log in to see MEM-43

Top referenced anti-CD59 antibody for Immunoprecipitation

Similar anti-CD59 Antibodies

Application / Reactivity Human
Blocking Antibody (Inhibition) 1 Antibodies
ELISA 68 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies
Flow Cytometry (FACS) 241 Antibodies
Functional Studies (Func) 18 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 8 Antibodies
Immunofluorescence (IF) 32 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 75 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 6 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 5 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 23 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 67 Antibodies
Immunoprecipitation (IP) 68 Antibodies

General

Antigen CD59 Antibodies
Epitope AA 1-101
(13), (7), (7), (7), (7), (6), (5), (5), (5), (4), (3), (2), (2), (1), (1), (1)
Reactivity Human
(340), (22), (19), (10), (9), (7), (2), (2), (2), (1), (1)
Host Rabbit
(249), (108), (25), (7), (1)
Clonality
Polyclonal
Conjugate This CD59 antibody is un-conjugated
(57), (48), (38), (10), (9), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Minimum Detection Limit 0.00975 ng/well
Application Immunoprecipitation (IP), ELISA, Western Blotting (WB)
(245), (137), (95), (78), (75), (70), (31), (29), (17), (14), (11), (6), (5), (3), (2), (2), (1)
Pubmed 4 references available
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Product Details anti-CD59 Antibody

Target Details CD59 Application Details Handling References for anti-CD59 Antibody (ABIN1999739) Images
Specificity Human CD59
Characteristics Produced in rabbits immunized with purified, recombinant Human CD59 (rh CD59, P13987-1, Met 1-Glu 101). CD59 specific IgG was purified by Human CD59 affinity chromatography.
Purification Affinity Purified
Sterility 0.2 μm filtered
Isotype IgG

Target Details CD59

Product Details anti-CD59 Antibody Application Details Handling References for anti-CD59 Antibody (ABIN1999739) Images back to top
Antigen
Alternative Name CD59 (CD59 Antibody Abstract)
Background CD59 glycoprotein, also known as 20 kDa homologous restriction factor, HRF20, MAC-inhibitory protein, Membrane attack complex inhibition factor, Membrane inhibitor of reactive lysis, MIC11, MIRL and CD59, is a cell membrane protein which contains one UPAR/Ly6 domain. CD59 is a small, highly glycosylated, GPI-linked protein, with a wide expression profile. The soluble form of CD59 from urine retains its specific complement binding activity, but exhibits greatly reduced ability to inhibit MAC assembly on cell membranes. CD59 is a potent inhibitor of the complement membrane attack complex (MAC) action. CD59 was first identified as a regulator of the terminal pathway of complement. It acts by binding to the C8 and/or C9 complements of the assembling MAC, thereby preventing incorporation of the multiple copies of C9 required for complete formation of the osmolytic pore. This inhibitor appears to be species-specific. CD59 is involved in signal transduction for T-cell activation complexed to a protein tyrosine kinase. Defects in CD59 are the cause of CD59 deficiency (CD59D).
UniProt P13987-1
Research Area Cardiovascular, Atherosclerosis, Hematopoietic Progenitors, Tyrosine Kinases, Innate Immunity, CD Antigens, Surface Receptors of Immune Cells
Pathways Complement System

Application Details

Product Details anti-CD59 Antibody Target Details CD59 Handling References for anti-CD59 Antibody (ABIN1999739) Images back to top
Application Notes ELISA ELISA: 0.1-0.2 μg/mL This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human CD59. The detection limit for Human CD59 is approximately 0.00975 ng/well.
Restrictions For Research Use only

Handling

Product Details anti-CD59 Antibody Target Details CD59 Application Details References for anti-CD59 Antibody (ABIN1999739) Images back to top
Buffer PBS with 5% trehalose
Preservative Without preservative
Handling Advice Avoid repeated freeze-thaw cycles.
Storage 4 °C/-20 °C/-80 °C
Storage Comment This antibody can be stored at 2°C-8°C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20°C to -70°C. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly.
Expiry Date 12 months

References for anti-CD59 Antibody (ABIN1999739)

Product Details anti-CD59 Antibody Target Details CD59 Application Details Handling Images back to top
Background publications

Heibeck, Ding, Opresko, Zhao, Schepmoes, Yang, Tolmachev, Monroe, Camp, Smith, Wiley, Qian: "An extensive survey of tyrosine phosphorylation revealing new sites in human mammary epithelial cells." in: Journal of proteome research, Vol. 8, Issue 8, pp. 3852-61, 2009

Picariello, Ferranti, Mamone, Roepstorff, Addeo: "Identification of N-linked glycoproteins in human milk by hydrophilic interaction liquid chromatography and mass spectrometry." in: Proteomics, Vol. 8, Issue 18, pp. 3833-47, 2008

Rudd, Morgan, Wormald, Harvey, van den Berg, Davis, Ferguson, Dwek: "The glycosylation of the complement regulatory protein, human erythrocyte CD59." in: The Journal of biological chemistry, Vol. 272, Issue 11, pp. 7229-44, 1997

Fletcher, Harrison, Lachmann, Neuhaus: "Structure of a soluble, glycosylated form of the human complement regulatory protein CD59." in: Structure (London, England : 1993), Vol. 2, Issue 3, pp. 185-99, 1994

Images

Product Details anti-CD59 Antibody Target Details CD59 Application Details Handling References for anti-CD59 Antibody (ABIN1999739) back to top
Supplier Images
Western Blotting (WB) image for anti-CD59 antibody (CD59) (AA 1-101) (ABIN1999739) anti-CD59 (CD59) (AA 1-101) antibody
Immunoprecipitation (IP) image for anti-CD59 antibody (CD59) (AA 1-101) (ABIN1999739) anti-CD59 (CD59) (AA 1-101) antibody (Image 2)