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anti-Mouse (Murine) CD59 antibody for Western Blotting

Recommended CD59 Antibody (supplied by: Log in to see )

Antigen
CD59 Antibodies
  • 1F5
  • 16.3A5
  • AA987121
  • Cd59
  • Cd59a
  • Cd59b
  • EJ16
  • EJ30
  • EL32
  • G344
  • HRF-20
  • HRF20
  • MAC-IP
  • MACIF
  • MACIP
  • MEM43
  • MIC11
  • MIN1
  • MIN2
  • MIN3
  • MIRL
  • MSK21
  • p18-20
  • protectin
Reactivity
Human, Mouse (Murine)
503
36
32
24
17
15
9
7
2
2
1
1
Host
Mouse
405
110
44
7
1
Clonality
Monoclonal
Conjugate
This CD59 antibody is un-conjugated
63
58
44
14
11
11
10
9
9
9
9
9
9
9
9
9
9
5
5
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
417
193
187
103
97
96
73
48
34
32
11
6
5
3
2
2
1
Supplier
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Catalog No. ABIN94203
$ 231.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.13341247 ABIN1077443 ELISA WB Rabbit IgG N-Term Log in to see
0.13341247 ABIN1174500 IHC ELISA WB Biotin Rabbit IgG AA 22-96 Log in to see Polyclonal
0.13341247 ABIN1174498 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 22-96 Log in to see Polyclonal
0.13341247 ABIN2434430 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
0.0011728023 ABIN400666 FACS IHC (p) IP WB Mouse IgG2b Log in to see MEM-43-5
0.0011728023 ABIN2469885 FACS IP IHC WB Mouse IgG2b Log in to see MEM-43-5
0.0011728023 ABIN1723362 FACS IHC (p) IP WB Mouse IgG2b Log in to see MEM-43-5
0.0011728023 ABIN2618690 WB FITC Rabbit IgG AA 22-96 Log in to see Polyclonal
0.0011728023 ABIN269032 FACS IHC (p) IP IHC WB Mouse IgG2b Log in to see MEM-43-5 1
0.0011728023 ABIN2860739 ELISA WB Rabbit N-Term Log in to see Polyclonal
0.0011728023 ABIN2962204 IHC (p) ELISA WB Rabbit IgG N-Term Log in to see Polyclonal
0.0011728023 ABIN2342600 IC IHC ELISA WB Rabbit IgG AA 22-96 Log in to see Polyclonal
0.0011728023 ABIN4294899 FACS IHC IHC (p) IP WB DyLight 350 Mouse IgG2b Log in to see MEM-43-5
0.0011728023 ABIN4294903 FACS IHC IHC (p) IP WB DyLight 405 Mouse IgG2b Log in to see MEM-43-5
0.0011728023 ABIN4294908 FACS IHC IHC (p) IP WB PerCP Mouse IgG2b Log in to see MEM-43-5
0.0011728023 ABIN4294916 FACS IHC IHC (p) IP WB DyLight 755 Mouse IgG2b Log in to see MEM-43-5
0.0011728023 ABIN4294920 FACS IHC IHC (p) IP WB DyLight 680 Mouse IgG2b Log in to see MEM-43-5
0.0011728023 ABIN4294912 FACS IHC IHC (p) IP WB Allophycocyanin Mouse IgG2b Log in to see MEM-43-5
0.0011728023 ABIN4294928 FACS IHC IHC (p) IP WB DyLight 405LS Mouse IgG2b Log in to see MEM-43-5
0.0011728023 ABIN4294931 FACS IHC IHC (p) IP WB PE Mouse IgG2b Log in to see MEM-43-5

Top referenced anti-CD59 antibody for Western Blotting

Similar anti-CD59 Antibodies

Application / Reactivity Mouse (Murine) Human
ELISA 11 Antibodies 69 Antibodies
Flow Cytometry (FACS) 20 Antibodies 393 Antibodies
Immunochromatography (IC) 1 Antibodies 1 Antibodies
Immunocytochemistry (ICC) 2 Antibodies
Immunohistochemistry (IHC) 20 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 21 Antibodies
Immunoprecipitation (IP) 20 Antibodies
Western Blotting (WB) 29 Antibodies
Blocking Antibody (Inhibition) 1 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies
Functional Studies (Func) 31 Antibodies

General

Antigen CD59 Antibodies
Reactivity Human, Mouse (Murine)
(503), (36), (32), (24), (17), (15), (9), (7), (2), (2), (1), (1)
Host Mouse
(405), (110), (44), (7), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD59 antibody is un-conjugated
(63), (58), (44), (14), (11), (11), (10), (9), (9), (9), (9), (9), (9), (9), (9), (9), (9), (5), (5), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
(417), (193), (187), (103), (97), (96), (73), (48), (34), (32), (11), (6), (5), (3), (2), (2), (1)
Pubmed 7 references available
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Product Details anti-CD59 Antibody

Target Details CD59 Application Details Handling References for anti-CD59 Antibody (ABIN94203) Images
Specificity The antibody MEM-43/5 reacts with well defined epitope (around L33) on CD59 (Protectin), a 18-20 kDa glycosylphosphatidylinositol (GPI)-anchored glycoprotein expressed on all hematopoietic cells, it is widely present on cells in all tissues. The MEM-43/5 does not compete with most other CD59 antibodies. HLDA V, WS Code AS S012
Purification Purified from ascites by protein-A affinity chromatography.
Purity > 95 % (by SDS-PAGE)
Immunogen Thymocytes and T lymphocytes
Clone MEM-43-5
Isotype IgG2b

Target Details CD59

Product Details anti-CD59 Antibody Application Details Handling References for anti-CD59 Antibody (ABIN94203) Images back to top
Antigen
Alternative Name CD59 (CD59 Antibody Abstract)
Background CD59 (Protectin) is a small (18-20 kDa) GPI-anchored ubiquitously expressed inhibitor of the membrane attack complex (MAC). It is thus the key regulator that preserves the autologous cells from terminal effector mechanism of the complement cascade. CD59 associates with C5b-8 complex and thereby counteracts appropriate formation of cytolytic pore within the plasma membrane. CD59 is also an low-affinity ligand of human CD2 and causes T cell costimulation.
Research Area Cardiovascular, Atherosclerosis, Hematopoietic Progenitors, Tyrosine Kinases, Innate Immunity, CD Antigens, Surface Receptors of Immune Cells
Pathways Complement System

Application Details

Product Details anti-CD59 Antibody Target Details CD59 Handling References for anti-CD59 Antibody (ABIN94203) Images back to top
Application Notes Western Blotting: Recommended dilution: 1-2 µg/mL
Positive control: mouse spleen
Application note: Non-reducing conditions.
Flow Cytometry: Recommended dilution: 1 µg/mL
Positive control: blood
Immunohistochemistry (paraffin sections) Recommended dilution: 5 µg/mL

Working concentrations should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-CD59 Antibody Target Details CD59 Application Details References for anti-CD59 Antibody (ABIN94203) Images back to top
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8 °C. Do not use after expiration date stamped on vial label.

References for anti-CD59 Antibody (ABIN94203)

Product Details anti-CD59 Antibody Target Details CD59 Application Details Handling Images back to top
Product cited in:

Drbal, Moertelmaier, Holzhauser, Muhammad, Fuertbauer, Howorka, Hinterberger, Stockinger, Schütz: "Single-molecule microscopy reveals heterogeneous dynamics of lipid raft components upon TCR engagement." in: International immunology, Vol. 19, Issue 5, pp. 675-84, 2007

Stulnig, Berger, Sigmund, Stockinger, Horejsí, Waldhäusl: "Signal transduction via glycosyl phosphatidylinositol-anchored proteins in T cells is inhibited by lowering cellular cholesterol." in: The Journal of biological chemistry, Vol. 272, Issue 31, pp. 19242-7, 1997

Bodian, Davis, Morgan, Rushmere: "Mutational analysis of the active site and antibody epitopes of the complement-inhibitory glycoprotein, CD59." in: The Journal of experimental medicine, Vol. 185, Issue 3, pp. 507-16, 1997

Background publications

Baalasubramanian, Harris, Donev, Mizuno, Omidvar, Song, Morgan: "CD59a is the primary regulator of membrane attack complex assembly in the mouse." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 173, Issue 6, pp. 3684-92, 2004

Menu, Tsai, Bothwell, Sims, Bierer: "CD59 costimulation of T cell activation. CD58 dependence and requirement for glycosylation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 153, Issue 6, pp. 2444-56, 1994

Rooney, Davies, Griffiths, Williams, Davies, Meri, Lachmann, Morgan: "The complement-inhibiting protein, protectin (CD59 antigen), is present and functionally active on glomerular epithelial cells." in: Clinical and experimental immunology, Vol. 83, Issue 2, pp. 251-6, 1991

Meri, Morgan, Davies, Daniels, Olavesen, Waldmann, Lachmann: "Human protectin (CD59), an 18,000-20,000 MW complement lysis restricting factor, inhibits C5b-8 catalysed insertion of C9 into lipid bilayers." in: Immunology, Vol. 71, Issue 1, pp. 1-9, 1990

Images

Product Details anti-CD59 Antibody Target Details CD59 Application Details Handling References for anti-CD59 Antibody (ABIN94203) back to top
Supplier Images
Western Blotting (WB) image for anti-CD59 antibody (CD59) (ABIN94203) Western Blotting analysis (non-reducing conditions) of whole cell lysate of HPB-ALL h...