anti-Human Complement Factor B antibody for Functional Studies

Recommended Complement Factor B Antibody (supplied by: Log in to see )

Antigen
Complement Factor B (CFB) Antibodies
  • bf
  • Bf-1
  • Brre-Bf
  • zgc:114032
  • Bf
  • Sp152
  • bfb-A
  • Bf B
  • LOC397725
  • fc04c04
  • wu:fc04c04
  • bf/c2
  • CFB
  • AHUS4
  • BF
  • BFD
  • CFAB
  • FB
  • FBI12
  • GBG
  • H2-Bf
  • PBF2
  • AI195813
  • AI255840
  • B
  • C2
  • Fb
  • Da1-24
  • complement factor B
  • zgc:158446
  • ATP synthase, H+ transporting, mitochondrial Fo complex, subunit s (factor B)
  • complement factor B-like
  • CFB
  • cfb
  • Bf
  • bfb-A
  • LOC397725
  • zgc:158446
  • bf/c2
  • Atp5s
  • Cfb
  • LOC100355944
Epitope
Fragment Ba
17
10
10
8
6
6
5
5
5
5
5
5
3
2
2
2
2
1
1
1
1
1
1
1
Reactivity
Human
177
50
47
2
1
1
1
1
1
Host
Mouse
129
56
16
7
Clonality
Monoclonal
Conjugate
This Complement Factor B antibody is un-conjugated
22
17
9
4
4
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Functional Studies (Func), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
139
124
54
29
23
20
16
13
12
11
9
6
5
5
4
3
2
1
1
Supplier
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Catalog No. ABIN181128
$ 577.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN2473064 Func IHC (fro) FACS ELISA WB Mouse IgG1 Fragment Ba Log in to see 014III-33-2-4-3 2
1 ABIN206673 Func FACS IHC (fro) ELISA WB Mouse IgG1 N-Term Log in to see
1 ABIN255394 Func FACS IHC (fro) IHC ELISA WB Mouse IgG1 Log in to see 014III-33-2-4-3
1 ABIN601090 Func IHC (fro) FACS IHC (p) ELISA WB Mouse IgG1 Log in to see
1 ABIN2965579 Func IHC (fro) FACS ELISA WB Mouse Fragment Ba Log in to see

Top referenced anti-Complement Factor B antibody for Functional Studies

Similar anti-Complement Factor B Antibodies

Application / Reactivity Human
ELISA 99 Antibodies
Enzyme Immunoassay (EIA) 13 Antibodies
Flow Cytometry (FACS) 30 Antibodies
Functional Studies (Func) 6 Antibodies
Immunoassay (IA) 3 Antibodies
Immunocytochemistry (ICC) 10 Antibodies
Immunodiffusion (ID) 5 Antibodies
Immunoelectrophoresis (IEP) 1 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 3 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 13 Antibodies
Immunohistochemistry (IHC) 39 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 12 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 23 Antibodies
Immunoprecipitation (IP) 14 Antibodies
Radioimmunoassay (RIA) 4 Antibodies
Simple Western (SimWes) 2 Antibodies
Western Blotting (WB) 109 Antibodies

General

Antigen Complement Factor B (CFB) Antibodies
Epitope Fragment Ba
(17), (10), (10), (8), (6), (6), (5), (5), (5), (5), (5), (5), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(177), (50), (47), (2), (1), (1), (1), (1), (1)
Host Mouse
(129), (56), (16), (7)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Complement Factor B antibody is un-conjugated
(22), (17), (9), (4), (4), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Functional Studies (Func), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
(139), (124), (54), (29), (23), (20), (16), (13), (12), (11), (9), (6), (5), (5), (4), (3), (2), (1), (1)
Pubmed 2 references available
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Product Details anti-Complement Factor B Antibody

Target Details Complement Factor B Application Details Handling ProductDetails: References for anti-Complement Factor B antibody (ABIN181128) Images
Specificity This antibody recognises the 30 kDa Ba fragment of 90 kDa complement factor B, present in blood serum.
Characteristics Synonyms: Properdin factor B, C3/C5 convertase, PBF2, CFB, BF, BFD, Glycine-rich beta glycoprotein
Purification Affinity chromatography on Protein A
Immunogen Purified human complement factor b.
Clone 014III-33-2-4-3
Isotype IgG1

Target Details Complement Factor B

Product Details anti-Complement Factor B Antibody Application Details Handling ProductDetails: References for anti-Complement Factor B antibody (ABIN181128) Images back to top
Antigen
Alternative Name Complement Factor B (CFB Antibody Abstract)
Background C3b associates with complement factor B, inducing conformational change. This enables complement factor D to cleave the N-terminal of complement factor B (the Ba subunit), leaving the 63 kDa Bb subunit associated with C3b, forming C3 convertase. Subunit Ba inhibits lymphocyte proliferation. Conversely, subunit Bb is involved in the proliferation of preactivated B lymphocytes.Synonyms: BF, BFD, C3/C5 convertase, CFB, Glycine-rich beta glycoprotein, PBF2, Properdin factor B
Gene ID 629
UniProt P00751
Research Area Coagulation
Pathways Complement System, Proton Transport, Ribonucleoside Biosynthetic Process

Application Details

Product Details anti-Complement Factor B Antibody Target Details Complement Factor B Handling ProductDetails: References for anti-Complement Factor B antibody (ABIN181128) Images back to top
Application Notes Western Blot. Functional Assays. Flow Cytometry. ELISA: This antibody binds both the Ba fragment and the whole protein so is not suitablefor all ELISA applications. Immunohistochemistry on frozen sections. Recommended Positiv Control: Kidney from post streptoccal glomerulonephritis patients.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-Complement Factor B Antibody Target Details Complement Factor B Application Details ProductDetails: References for anti-Complement Factor B antibody (ABIN181128) Images back to top
Concentration 1.0mg/mL
Buffer Borate buffered saline pH 8.2 - 8.4 containing 0.02 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Expiry Date 12 months

ProductDetails: References for anti-Complement Factor B antibody (ABIN181128)

Product Details anti-Complement Factor B Antibody Target Details Complement Factor B Application Details Handling Images back to top
Background publications

Ponnuraj, Xu, Macon, Moore, Volanakis, Narayana: "Structural analysis of engineered Bb fragment of complement factor B: insights into the activation mechanism of the alternative pathway C3-convertase." in: Molecular cell, Vol. 14, Issue 1, pp. 17-28, 2004

Xu, Narayana, Volanakis: "Structural biology of the alternative pathway convertase." in: Immunological reviews, Vol. 180, pp. 123-35, 2001