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anti-Mouse (Murine) Complement Factor H antibody for Enzyme Immunoassay

Recommended Complement Factor H Antibody (supplied by: Log in to see )

Antigen
Complement Factor H (CFH) Antibodies
  • AHUS1
  • AMBP1
  • ARMD4
  • ARMS1
  • CFH
  • CFHL3
  • fH
  • Fh
  • FH
  • FHL1
  • HF
  • HF1
  • HF2
  • HUS
  • im:7147412
  • Mud-1
  • NOM
  • Sas-1
  • Sas1
  • si:ch211-207o17.2
Reactivity
Horse (Equine), Cat (Feline), Human, Mouse (Murine), Rat (Rattus)
204
29
23
2
2
2
2
1
1
Host
Sheep
93
71
31
26
2
1
Clonality
Polyclonal
Conjugate
This Complement Factor H antibody is un-conjugated
17
11
5
3
3
3
2
2
2
2
2
2
2
2
2
Application
Enzyme Immunoassay (EIA), Immunodiffusion (ID)
139
124
43
39
31
22
21
18
12
11
8
6
5
4
2
1
1
1
1
1
1
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Catalog No. ABIN113017
$ 456.50
Plus shipping costs $45.00

Top referenced anti-Complement Factor H antibody for Enzyme Immunoassay

Similar anti-Complement Factor H Antibodies

Application / Reactivity Horse (Equine) Cat (Feline) Human Mouse (Murine) Rat (Rattus)
Western Blotting (WB) 1 Antibodies 1 Antibodies 132 Antibodies 14 Antibodies 10 Antibodies
Radioimmunoassay (RIA) 5 Antibodies
Radial Immunodiffusion (RID) 1 Antibodies
Ouchterlony Double Diffusion (ODD) 1 Antibodies
Immunoprecipitation (IP) 31 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 37 Antibodies 4 Antibodies 5 Antibodies
Immunohistochemistry (IHC) 45 Antibodies 1 Antibodies 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies 1 Antibodies 9 Antibodies

General

Antigen Complement Factor H (CFH) Antibodies
Reactivity Horse (Equine), Cat (Feline), Human, Mouse (Murine), Rat (Rattus)
(204), (29), (23), (2), (2), (2), (2), (1), (1)
Host Sheep
(93), (71), (31), (26), (2), (1)
Clonality
Polyclonal
Conjugate This Complement Factor H antibody is un-conjugated
(17), (11), (5), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application Enzyme Immunoassay (EIA), Immunodiffusion (ID)
(139), (124), (43), (39), (31), (22), (21), (18), (12), (11), (8), (6), (5), (4), (2), (1), (1), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-Complement Factor H Antibody

Target Details Complement Factor H Application Details Handling References for anti-Complement Factor H Antibody (ABIN113017) Images
Specificity Gives a single arc when tested by IEP against Human plasma. Identity is confirmed by double diffusion (Ouchterlony) vs. Human plasma and a known anti-Human Factor H. The cross reactivity of this product against the sera of the species listed below has been assessed by Double Diffusion using 10 μL of antisera against 10 μL of species sera. These results are intended as a guideline only. It is possible that cross-reactivity against species listed as negative above may be observed when using different test conditions or techniques.
Cross-Reactivity (Details) Species reactivity (tested):Cat + Chicken - Cow - Dog + Guinea Pig + Horse + Human + Mouse + Pig - Rabbit - Rat + Goat -
Purification Delipidation and Absorbtion to monospecificity by use of solid-phase absorbants. The titer is adjusted so that inter-batch variation is within 10%. Product is 0.2 µm filtered.
Immunogen Human 155 kDa Factor H purified from plasma, shown to be pure by IEP.

Target Details Complement Factor H

Product Details anti-Complement Factor H Antibody Application Details Handling References for anti-Complement Factor H Antibody (ABIN113017) Images back to top
Antigen
Background The complement Factor H protein is secreted into the bloodstream and acts in the regulation of complement activation. Mutations leading to changes in this protein have been linked with HUS (hemolytic-uremic syndrome) and chronic hypocomplementemic nephropathy.Synonyms: CFH, H factor 1, HF, HF1, HF2
Gene ID 3075
NCBI Accession NP_000177
UniProt P08603
Research Area Coagulation, Complement Factors
Pathways Complement System

Application Details

Product Details anti-Complement Factor H Antibody Target Details Complement Factor H Handling References for anti-Complement Factor H Antibody (ABIN113017) Images back to top
Application Notes Suitable for use in a variety of Gel techniques including Radial Immunodiffusion (RID),Double Diffusion and Immunoelectrophoresis (IEP). RID: 1 μL antiserum/cm2 gel vs.5 μL neat-1/10 dilution Human plasma. Double Diffusion: 10 μL antiserum vs.10 μL plasma. IEP: 100 μL antiserum vs.5 μL plasma. The use of 3 % PEG 6000 with 1.2 % agarose in a suitable buffer (such as TBE or tris-barbital pH > 8.2) is recommended.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-Complement Factor H Antibody Target Details Complement Factor H Application Details References for anti-Complement Factor H Antibody (ABIN113017) Images back to top
Format Liquid
Concentration 9.5 mg/mL (OD280 nm, E1% = 14.5)
Buffer Glycine buffered saline, pH 7.4 with 0.09 % Sodium Azide, 0.1 % EACA, 0.01 % Benzamidine and 1 mM EDTA.
Preservative Sodium azide, Benzamidine, EACA
Precaution of Use This product contains Sodium azide, Benzamidine and EACA: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-Complement Factor H Antibody (ABIN113017)

Product Details anti-Complement Factor H Antibody Target Details Complement Factor H Application Details Handling Images back to top
Background publications

Chung, Liszewski, Nybakken, Davis, Townsend, Fremont, Atkinson, Diamond: "West Nile virus nonstructural protein NS1 inhibits complement activation by binding the regulatory protein factor H." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 103, Issue 50, pp. 19111-6, 2006