anti-Rat (Rattus) Complement Factor H antibody for Western Blotting

Recommended Complement Factor H Antibody (supplied by: Log in to see )

Antigen
Complement Factor H (CFH) Antibodies
  • CFH
  • im:7147412
  • si:ch211-207o17.2
  • Mud-1
  • NOM
  • Sas-1
  • Sas1
  • AHUS1
  • AMBP1
  • ARMD4
  • ARMS1
  • CFHL3
  • FH
  • FHL1
  • HF
  • HF1
  • HF2
  • HUS
  • Fh
  • fH
  • complement factor H
  • complement component factor h
  • CFH
  • cfh
  • Cfh
Reactivity
Human, Mouse (Murine), Rat (Rattus)
242
35
29
4
4
3
3
2
2
Host
Rabbit
103
102
32
25
2
1
Clonality
Polyclonal
Conjugate
This Complement Factor H antibody is un-conjugated
20
14
6
5
4
3
3
3
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Immunochromatography (IC), Immunofluorescence (IF), Western Blotting (WB)
177
152
62
43
37
27
26
22
12
11
10
10
6
5
4
2
1
1
1
1
1
Supplier
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Available images

Catalog No. ABIN2966633
$ 487.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.1588345 ABIN3023097 IHC WB Rabbit IgG Log in to see Polyclonal
3.1588345 ABIN1858382 ICC IHC IP WB Rabbit IgG AA 975-1217 Log in to see Polyclonal
1 ABIN113379 ID IHC (fro) WB Sheep IgG Log in to see Polyclonal
1 ABIN2619171 ELISA WB Biotin Rabbit IgG AA 975-1217 Log in to see Polyclonal
1 ABIN2619174 ELISA WB FITC Rabbit IgG AA 975-1217 Log in to see Polyclonal
1 ABIN1421104 IHC (p) WB HRP Rabbit IgG Log in to see Polyclonal
1 ABIN2619168 ELISA WB Rabbit IgG AA 975-1217 Log in to see Polyclonal
1 ABIN1403233 IHC (p) WB Biotin Rabbit IgG Log in to see Polyclonal
1 ABIN2878086 IHC WB Rabbit Log in to see Polyclonal
1 ABIN1387098 IF (p) IHC (p) WB Rabbit IgG Log in to see Polyclonal
1 ABIN5540274 EIA IHC (fro) WB Mouse IgG1 Log in to see 1A2
1 ABIN3201472 ICC IHC (fro) IHC (p) ELISA WB Mouse IgG Log in to see
1 ABIN5540273 EIA IHC (fro) WB FITC Mouse IgG1 Log in to see 1A2
1 ABIN2896779 ELISA IF/ICC IHC WB Rabbit AA 975-1217 Log in to see Polyclonal

Similar anti-Complement Factor H Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
ELISA 5 Antibodies 6 Antibodies
Enzyme Immunoassay (EIA) 3 Antibodies 3 Antibodies
Immunochromatography (IC) 1 Antibodies 1 Antibodies
Immunocytochemistry (ICC) 2 Antibodies 1 Antibodies
Immunodiffusion (ID) 4 Antibodies 4 Antibodies
Immunofluorescence (IF) 1 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 1 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 13 Antibodies
Immunohistochemistry (IHC) 4 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 4 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 4 Antibodies
Immunoprecipitation (IP) 1 Antibodies
Western Blotting (WB) 15 Antibodies
Flow Cytometry (FACS) 2 Antibodies
Immunoassay (IA) 2 Antibodies

General

Antigen Complement Factor H (CFH) Antibodies
Reactivity Human, Mouse (Murine), Rat (Rattus)
(242), (35), (29), (4), (4), (3), (3), (2), (2)
Host Rabbit
(103), (102), (32), (25), (2), (1)
Clonality
Polyclonal
Conjugate This Complement Factor H antibody is un-conjugated
(20), (14), (6), (5), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunochromatography (IC), Immunofluorescence (IF), Western Blotting (WB)
(177), (152), (62), (43), (37), (27), (26), (22), (12), (11), (10), (10), (6), (5), (4), (2), (1), (1), (1), (1), (1)
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Product Details anti-Complement Factor H Antibody

Target Details Complement Factor H Application Details Handling Images
Specificity Recognizes endogenous levels of FHL-1 protein.
Characteristics Rabbit polyclonal antibody to FHL-1
Purification The antibody was purified by immunogen affinity chromatography.
Immunogen Recombinant full length protein of human FHL-1

Target Details Complement Factor H

Product Details anti-Complement Factor H Antibody Application Details Handling Images back to top
Antigen
Alternative Name FHL-1 (CFH Antibody Abstract)
Background SLIM1, Four and a half LIM domains protein 1, FHL-1, Skeletal muscle LIM-protein 1, SLIM, SLIM-1
Gene ID 2273, 14199, 25177
UniProt Q13642, P97447, Q9WUH4
Pathways Complement System, Cellular Response to Molecule of Bacterial Origin

Application Details

Product Details anti-Complement Factor H Antibody Target Details Complement Factor H Handling Images back to top
Application Notes WB (1:500 - 1:2000), IF/IC (1:50 - 1:100)
Restrictions For Research Use only

Handling

Product Details anti-Complement Factor H Antibody Target Details Complement Factor H Application Details Images back to top
Format Liquid
Buffer Liquid in 0.42 % Potassium phosphate, 0.87 % Sodium chloride, pH 7.3, 30 % glycerol, and 0.01 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
Expiry Date 12 months

Images

Product Details anti-Complement Factor H Antibody Target Details Complement Factor H Application Details Handling back to top
Supplier Images
Immunofluorescence (IF) image for anti-Complement Factor H antibody (CFH) (ABIN2966633) Immunofluorescent analysis of FHL-1 staining in Hela cells. Formalin-fixed cells were...
Western Blotting (WB) image for anti-Complement Factor H antibody (CFH) (ABIN2966633) Western blot analysis of FHL-1 expression in 22RV1 (A), MCF7 (B), mouse kidney (C) wh...
'Independent Validation' Badge
Antigen Commplement Factor H
Lot Number AL1F04A
Method validated Immunofluorescence
Positive Control MRL/lpr lupus mice (14 weeks of age)
Negative Control FH knockout MRL/lpr lupus mice
Notes Passed. ABIN2966633 works well on murine kidney samples and shows the expected CFH1 localization.
Primary Antibody ABIN2966633
Secondary Antibody goat anti-rabbit rhodamine conjugated antibody (ThermoFisher Scientific, T-2769, lot 6251)
Protocol
  • Prepare kidneys of wt and complement factor H ko MRL/lpr lupus mice of 14 weeks of age.
  • Snap-freeze kidneys in 2-methylbutane cooled on dry ice and keep at −80°C until use.
  • Sections were cut at 8µm using the cryostat.
  • Dry sections for 30min at RT.
  • Hydrate sections in PBS 2x for 15min at RT.
  • Fix cryostat sections with 4% paraformaldehyde (PFA) for 15min at RT.
  • Wash sections 2x with PBS for 15min at RT.
  • Incubate sections with primary rabbit anti-Complement Factor H antibody (antibodies-online, ABIN2966633, AL1F04A) diluted 1:100 in PBS ON at 4°C.
  • Wash sections 2x for 15min with PBS.
  • Incubate sections with secondary goat anti-rabbit rhodamine conjugated antibody (ThermoFisher Scientific, T-2769, lot 6251) diluted 1:100 in PBS for 30min at RT.
  • Wash sections 2x for 15min with PBS.
  • Incubate sections with ActinGreen 488 ReadyProbes Reagent (ThermoFisher Scientific, R37122, lot 1700569) diluted 1:100 in PBS for 30min at RT.
  • Wash sections 2x for 15min with PBS.
  • Image acquisition on a Zeiss microscope (Carl Zeiss, Oberkochen, Germany).
Validation Images
Immunofluorescence validation image for anti-Complement Factor H (CFH) antibody (ABIN2966633) IF staining with ABIN2966633 of kidneys harvested from 14 week old MRL/lpr mice suffi...