anti-Rat (Rattus) Complement Factor H antibody for Western Blotting

Recommended Complement Factor H Antibody (supplied by: Log in to see )

Antigen
Complement Factor H (CFH) Antibodies
  • CFH
  • im:7147412
  • si:ch211-207o17.2
  • Mud-1
  • NOM
  • Sas-1
  • Sas1
  • AHUS1
  • AMBP1
  • ARMD4
  • ARMS1
  • CFHL3
  • FH
  • FHL1
  • HF
  • HF1
  • HF2
  • HUS
  • Fh
  • fH
  • complement factor H
  • complement component factor h
  • CFH
  • cfh
  • Cfh
Reactivity
Mouse (Murine), Rat (Rattus), Human
241
34
28
4
4
3
3
2
2
Host
Rabbit
103
102
32
24
2
1
Clonality
Polyclonal
Conjugate
This Complement Factor H antibody is un-conjugated
20
14
6
5
4
3
3
3
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), Western Blotting (WB)
177
151
60
43
37
27
26
22
12
11
10
10
6
5
4
2
1
1
1
1
1
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Available images

Catalog No. ABIN3023097
$ 302.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
2.9061391 ABIN1858382 ICC IHC IP WB Rabbit IgG AA 975-1217 Log in to see Polyclonal
1 ABIN113379 ID IHC (fro) WB Sheep IgG Log in to see Polyclonal
1 ABIN1421104 IHC (p) WB HRP Rabbit IgG Log in to see Polyclonal
1 ABIN1403233 IHC (p) WB Biotin Rabbit IgG Log in to see Polyclonal
1 ABIN1387098 IF (p) IHC (p) WB Rabbit IgG Log in to see Polyclonal
1 ABIN2619174 ELISA WB FITC Rabbit IgG AA 975-1217 Log in to see Polyclonal
1 ABIN2619171 ELISA WB Biotin Rabbit IgG AA 975-1217 Log in to see Polyclonal
1 ABIN2619168 ELISA WB Rabbit IgG AA 975-1217 Log in to see Polyclonal
1 ABIN2878086 IHC WB Rabbit Log in to see Polyclonal
1 ABIN5540274 EIA IHC (fro) WB Mouse IgG1 Log in to see 1A2
1 ABIN3201472 ICC IHC (fro) IHC (p) ELISA WB Mouse IgG Log in to see
1 ABIN5540273 EIA IHC (fro) WB FITC Mouse IgG1 Log in to see 1A2
1 ABIN2896779 ELISA IF/ICC IHC WB Rabbit AA 975-1217 Log in to see Polyclonal

General

Antigen Complement Factor H (CFH) Antibodies
Reactivity Mouse (Murine), Rat (Rattus), Human
(241), (34), (28), (4), (4), (3), (3), (2), (2)
Host Rabbit
(103), (102), (32), (24), (2), (1)
Clonality
Polyclonal
Conjugate This Complement Factor H antibody is un-conjugated
(20), (14), (6), (5), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (IHC), Western Blotting (WB)
(177), (151), (60), (43), (37), (27), (26), (22), (12), (11), (10), (10), (6), (5), (4), (2), (1), (1), (1), (1), (1)
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Product Details anti-Complement Factor H Antibody

Target Details Complement Factor H Application Details Handling Images
Purification Affinity purification
Immunogen Recombinant protein of human CFH
Isotype IgG

Target Details Complement Factor H

Product Details anti-Complement Factor H Antibody Application Details Handling Images back to top
Antigen
Alternative Name CFH (CFH Antibody Abstract)
Background CFH (Complement factor H) is a member of the Regulator of Complement Activation (RCA) gene cluster and a protein with twenty short concensus repeat (SCR) domains. This protein is secreted into the bloodstream and has an essential role in the regulation of complement activation, restricting this innate defense mechanism to microbial infections. Mutations in this CFH have been associated with hemolytic-uremic syndrome (HUS) and chronic hypocomplementemic nephropathy. 1. Entrez Gene: gene-centered information at NCBI. Nucleic Acids Res. 2005 Jan 1, 33:D54-8.
Synonyms: ARMD4, ARMS1, CFHL3, FH, FHL1, HF, HF1, HF2, HUS, MGC88246
Gene ID 3075
UniProt P08603
Pathways Complement System, Cellular Response to Molecule of Bacterial Origin

Application Details

Product Details anti-Complement Factor H Antibody Target Details Complement Factor H Handling Images back to top
Application Notes Recommended dilutions: WB: 1:500-1:2000, IHC: 1:50-1:200
Restrictions For Research Use only

Handling

Product Details anti-Complement Factor H Antibody Target Details Complement Factor H Application Details Images back to top
Format Liquid
Buffer PBS with 0.02 % sodium azide, 50 % glycerol, pH 7.3
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze / thaw cycles
Storage -20 °C/-80 °C

Images

Product Details anti-Complement Factor H Antibody Target Details Complement Factor H Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-Complement Factor H (CFH) antibody (ABIN3023097) Western blot analysis of extracts of HepG2 cell line, using CFH antibody.
'Independent Validation' Badge
Antigen Commplement Factor H
Lot Number 4390101
Method validated Immunofluorescence
Positive Control MRL/lpr lupus mice (14 weeks of age)
Negative Control FH knockout MRL/lpr lupus mice
Notes Passed. The complement factor H antibody ABIN3023097 works very well on murine kidney samples and shows the expected CFH localization and strong staining.
Primary Antibody ABIN3023097
Secondary Antibody goat anti-rabbit rhodamine conjugated antibody (ThermoFisher Scientific, T-2769, lot 6251)
Protocol
  • Prepare kidneys of wt and complement factor H ko MRL/lpr lupus mice of 14 weeks of age.
  • Snap-freeze kidneys in 2-methylbutane cooled on dry ice and keep at −80°C until use.
  • Sections were cut at 8µm using the cryostat.
  • Dry sections for 30min at RT.
  • Hydrate sections in PBS 2x for 15min at RT.
  • Fix cryostat sections with 4% paraformaldehyde (PFA) for 15min at RT.
  • Wash sections 2x with PBS for 15min at RT.
  • Incubate sections with primary rabbit anti-Complement Factor H antibody (antibodies-online, ABIN3023097, 0004390101) diluted 1:100 in PBS ON at 4°C.
  • Wash sections 2x for 15min with PBS.
  • Incubate sections with secondary goat anti-rabbit rhodamine conjugated antibody (ThermoFisher Scientific, T-2769, lot 6251) diluted 1:100 in PBS for 30min at RT.
  • Wash sections 2x for 15min with PBS.
  • Incubate sections with ActinGreen 488 ReadyProbes Reagent (ThermoFisher Scientific, R37122, lot 1700569) diluted 1:100 in PBS for 30min at RT.
  • Wash sections 2x for 15min with PBS.
  • Image acquisition on a Zeiss microscope (Carl Zeiss, Oberkochen, Germany).
Validation Images
Immunofluorescence validation image for anti-Complement Factor H (CFH) antibody (ABIN3023097) IF staining with ABIN3023097 of kidneys harvested from 14 week old MRL/lpr mice suffi...