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anti-Human Complement Factor I antibody for Enzyme Immunoassay

Recommended Complement Factor I Antibody (supplied by: Log in to see )

Antigen
Complement Factor I (CFI) Antibodies
  • AHUS3
  • ahus3
  • c3b-ina
  • C3b-INA
  • c3bc4bi
  • C3BINA
  • c3bina
  • Cfi
  • cfi
  • CFI
  • factor I
  • FI
  • gb:ai721528
  • IF
  • KAF
  • kaf
  • MGC53615
Reactivity
Human
104
1
Host
Mouse
75
27
2
1
Clonality
Monoclonal
Conjugate
This Complement Factor I antibody is conjugated to FITC
4
2
Application
Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Immunoprecipitation (IP), Radioimmunoassay (RIA), Western Blotting (WB)
89
38
12
9
9
8
7
6
5
3
2
2
2
1
Supplier
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Catalog No. ABIN951648
$ 308.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008857296 ABIN951647 EIA FACS IP RIA WB Biotin Mouse IgG1 Log in to see OX-21 4
0.0008857296 ABIN181115 EIA Func IHC (fro) WB Mouse IgG1 Log in to see 3R-8 2
0.0008857296 ABIN2487190 EIA FACS IHC (p) IP WB Mouse IgG1 Log in to see OX-21
0.0008857296 ABIN118974 EIA IP RIA WB Mouse IgG1 Log in to see OX-21 1
0.0008857296 ABIN1106813 EIA Mouse IgG2b Log in to see KT22
0.0008857296 ABIN1106815 EIA IHC (fro) WB Rabbit Log in to see Polyclonal
0.0008857296 ABIN1803649 EIA RIA Biotin Rabbit IgG Log in to see Polyclonal
0.0008857296 ABIN1803648 EIA RIA Rabbit IgG Log in to see Polyclonal

Top referenced anti-Complement Factor I antibody for Enzyme Immunoassay

Similar anti-Complement Factor I Antibodies

Application / Reactivity Human
ELISA 40 Antibodies
Enzyme Immunoassay (EIA) 9 Antibodies
Flow Cytometry (FACS) 9 Antibodies
Functional Studies (Func) 3 Antibodies
Immunocytochemistry (ICC) 2 Antibodies
Immunodiffusion (ID) 2 Antibodies
Immunofluorescence (IF) 2 Antibodies
Immunohistochemistry (IHC) 10 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 5 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 7 Antibodies
Immunoprecipitation (IP) 14 Antibodies
Radioimmunoassay (RIA) 10 Antibodies
Western Blotting (WB) 92 Antibodies

General

Antigen Complement Factor I (CFI) Antibodies
Reactivity Human
(104), (1)
Host Mouse
(75), (27), (2), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Complement Factor I antibody is conjugated to FITC
(4), (2)
Application Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Immunoprecipitation (IP), Radioimmunoassay (RIA), Western Blotting (WB)
(89), (38), (12), (9), (9), (8), (7), (6), (5), (3), (2), (2), (2), (1)
Pubmed 1 reference available
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Product Details anti-Complement Factor I Antibody

Target Details Complement Factor I Application Details Handling References for anti-Complement Factor I Antibody (ABIN951648) Images
Specificity Anti-human factor I (C3b inactivator) monoclonal antibody clone OX-21 recognizes human complement protein factor I. Factor I has two polypeptide chains, 56 kDa and 36 kDa. This antibody detects only the 56 kDa chain. This clone has been reported not to cross react with rat.
Purification Affinity chromatography on Protein G
Immunogen Human complement Factor I Donor: Immunized FI cross DBA/2 x BALB/c mouse spleen cells Fusion Partner: NS-1 mouse myeloma
Clone OX-21
Isotype IgG1

Target Details Complement Factor I

Product Details anti-Complement Factor I Antibody Application Details Handling References for anti-Complement Factor I Antibody (ABIN951648) Images back to top
Antigen
Alternative Name Complement Factor I (CFI Antibody Abstract)
Background Factor I is a serine protease with a high degree of specificity for C3b and C4b. It requires protein cofactors for cleavage of these complement protein breakdown products: Factor H, CR1 and MCP are required for C3b cleavage and C4bp or CR1 for C4b cleavage.Synonyms: AHUS3, C3B/C4B inactivator, C3BINA, C3b-INA, CFI, IF, KAF
Gene ID 3426
NCBI Accession NP_000195
UniProt P05156
Research Area Coagulation
Pathways Complement System

Application Details

Product Details anti-Complement Factor I Antibody Target Details Complement Factor I Handling References for anti-Complement Factor I Antibody (ABIN951648) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-Complement Factor I Antibody Target Details Complement Factor I Application Details References for anti-Complement Factor I Antibody (ABIN951648) Images back to top
Concentration 0,1 mg/mL
Buffer PBS containing 0.02 % sodium azide (NaN3) as preservative and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/mL.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer.

References for anti-Complement Factor I Antibody (ABIN951648)

Product Details anti-Complement Factor I Antibody Target Details Complement Factor I Application Details Handling Images back to top
Background publications

Hsiung, Barclay, Brandon, Sim, Porter: "Purification of human C3b inactivator by monoclonal-antibody affinity chromatography." in: The Biochemical journal, Vol. 203, Issue 1, pp. 293-8, 1982