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anti-Monkey CR1 antibody for Western Blotting

Recommended CR1 Antibody (supplied by: Log in to see )

Antigen
Complement Component Receptor 1 (CD35) (CR1) Antibodies
  • C3BR
  • C3DR
  • C4BR
  • CD21
  • CD35
  • Cr-1
  • Cr-2
  • Cr1
  • KN
Reactivity
Baboon, Human, Monkey
193
16
13
10
10
6
5
2
Host
Mouse
167
28
2
Clonality
Monoclonal
Conjugate
This CR1 antibody is un-conjugated
33
30
10
3
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
148
75
59
42
41
38
21
20
11
11
11
4
4
1
1
1
1
1
1
1
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Catalog No. ABIN118609
$ 253.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0011806369 ABIN118608 FACS IHC (fro) IHC (p) WB Mouse IgG1 Log in to see E11 4
0.0011806369 ABIN319720 FACS IHC (fro) IHC (p) WB Mouse IgG1 Log in to see E11 2
0.0011806369 ABIN501189 FACS IHC (fro) IHC (p) WB Mouse IgG1 Log in to see E11 2
0.0011806369 ABIN118606 FACS IHC (fro) IHC (p) WB Mouse IgG1 Log in to see E11 4
0.0011806369 ABIN1723986 IHC (fro) FACS IHC (p) WB Mouse IgG1 Log in to see E11
0.0011806369 ABIN3199787 FACS ICC IHC IP WB FITC Mouse IgG1 Log in to see E11
0.0011806369 ABIN3199788 FACS ICC IHC IP WB PE Mouse IgG1 Log in to see E11
0.0011806369 ABIN3199786 FACS ICC IHC IP WB Mouse IgG1 Log in to see E11
0.0011806369 ABIN3037175 ICC FACS IF IP IHC WB Mouse IgG1 Log in to see
0.0011806369 ABIN3037176 ICC FACS IF IP IHC WB FITC Mouse IgG1 Log in to see
0.0011806369 ABIN3037177 ICC FACS IF IP IHC WB PE Mouse IgG1 Log in to see

Top referenced anti-CR1 antibody for Western Blotting

Similar anti-CR1 Antibodies

Application / Reactivity Monkey Human
Flow Cytometry (FACS) 17 Antibodies 147 Antibodies
Immunocytochemistry (ICC) 6 Antibodies 21 Antibodies
Immunofluorescence (IF) 3 Antibodies 20 Antibodies
Immunohistochemistry (IHC) 6 Antibodies 43 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 7 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 7 Antibodies
Immunoprecipitation (IP) 6 Antibodies
Western Blotting (WB) 12 Antibodies
ELISA 11 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies
Functional Studies (Func) 1 Antibodies
Immunoassay (IA) 1 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies

General

Antigen Complement Component Receptor 1 (CD35) (CR1) Antibodies
Reactivity Baboon, Human, Monkey
(193), (16), (13), (10), (10), (6), (5), (2)
Host Mouse
(167), (28), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CR1 antibody is un-conjugated
(33), (30), (10), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(148), (75), (59), (42), (41), (38), (21), (20), (11), (11), (11), (4), (4), (1), (1), (1), (1), (1), (1), (1)
Pubmed 4 references available
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Product Details anti-CR1 Antibody

Target Details CR1 Application Details Handling References for anti-CR1 Antibody (ABIN118609) Images
Purification Affinity Chromatography on Protein A
Immunogen Human acute monocytic leukaemia cells. Remarks: Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
Clone E11
Isotype IgG1

Target Details CR1

Product Details anti-CR1 Antibody Application Details Handling References for anti-CR1 Antibody (ABIN118609) Images back to top
Antigen
Alternative Name CD35 / C3BR (CR1 Antibody Abstract)
Background CD35 is a membrane glycoprotein found on peripheral blood cells, glomerular podocytes, and follicular dendritic cells. The protein is a receptor for complement components C3b and C4b and regulates the activity of the complement cascade. Variation in this protein is the basis of the Knops blood group system. The two most common alleles, F and S, differ by 8 exons and are thought to be the result of an unequal crossover event. A secreted form of the protein present in plasma has been described, but its full length nature has not been determined.Synonyms: C3b/C4b receptor, CR1, Complement receptor type 1
Gene ID 9606
UniProt P17927
Research Area CD Antigens, Surface Receptors of Immune Cells
Pathways Complement System

Application Details

Product Details anti-CR1 Antibody Target Details CR1 Handling References for anti-CR1 Antibody (ABIN118609) Images back to top
Application Notes Flow Cytometry: 1/50 - 1/200, Use 10 μL of the suggested working dilution to label 10^6 cells or 100 μL whole blood. Immunohistochemistry on frozen sections: 1 μg/mL - 10 μg/mL. Immunohistochemistry on paraffin sections: 1 μg/mL - 10 μg/mL, This product requires antigen retrieval using heat treatment prior to staining of paraffin sections, Sodium citrate buffer pH 6.0 is recommended for this purpose. Recommended Positive Control: Human Tonsil.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-CR1 Antibody Target Details CR1 Application Details References for anti-CR1 Antibody (ABIN118609) Images back to top
Concentration 1.0 mg/mL
Buffer PBS, pH 7.4, 0.09 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-CR1 Antibody (ABIN118609)

Product Details anti-CR1 Antibody Target Details CR1 Application Details Handling Images back to top
Background publications

Yoshino, Ami, Terao, Tashiro, Honda: "Upgrading of flow cytometric analysis for absolute counts, cytokines and other antigenic molecules of cynomolgus monkeys (Macaca fascicularis) by using anti-human cross-reactive antibodies." in: Experimental animals / Japanese Association for Laboratory Animal Science, Vol. 49, Issue 2, pp. 97-110, 2000

Sopper, Stahl-Hennig, Demuth, Johnston, Dörries, ter Meulen: "Lymphocyte subsets and expression of differentiation markers in blood and lymphoid organs of rhesus monkeys." in: Cytometry, Vol. 29, Issue 4, pp. 351-62, 1998

Birmingham, Logar, Shen, Chen: "The baboon erythrocyte complement receptor is a glycophosphatidylinositol-linked protein encoded by a homologue of the human CR1-like genetic element." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 157, Issue 6, pp. 2586-92, 1996

Hogg, Ross, Jones, Slusarenko, Walport, Lachmann: "Identification of an anti-monocyte monoclonal antibody that is specific for membrane complement receptor type one (CR1)." in: European journal of immunology, Vol. 14, Issue 3, pp. 236-43, 1984

Images

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Supplier Images
Flow Cytometry (FACS) image for anti-CR1 antibody (Complement Component Receptor 1 (CD35)) (ABIN118609) ABIN118609 staining of human peripheral blood monocytes.