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anti-Mouse (Murine) CR1 antibody for Flow Cytometry

Recommended CR1 Antibody (supplied by: Log in to see )

Antigen
Complement Component Receptor 1 (CD35) (CR1) Antibodies
  • C3BR
  • C3DR
  • C4BR
  • CD21
  • CD35
  • Cr-1
  • Cr-2
  • Cr1
  • KN
Reactivity
Mouse (Murine)
194
17
14
10
10
5
5
2
Host
Rat
168
28
1
Clonality
Monoclonal
Conjugate
This CR1 antibody is conjugated to Biotin
33
30
9
3
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS)
148
76
60
42
41
38
21
20
11
11
11
4
4
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN2689207
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0010476487 ABIN967625 BR FACS IP WB Rat IgG2a, kappa Log in to see 8C12 11

Top referenced anti-CR1 antibody for Flow Cytometry

Similar anti-CR1 Antibodies

Application / Reactivity Mouse (Murine)
Blocking Reagent (BR) 1 Antibodies
ELISA 2 Antibodies
Flow Cytometry (FACS) 2 Antibodies
Immunohistochemistry (IHC) 2 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunoprecipitation (IP) 1 Antibodies
Western Blotting (WB) 3 Antibodies

General

Antigen Complement Component Receptor 1 (CD35) (CR1) Antibodies
Reactivity Mouse (Murine)
(194), (17), (14), (10), (10), (5), (5), (2)
Host Rat
(168), (28), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CR1 antibody is conjugated to Biotin
(33), (30), (9), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS)
(148), (76), (60), (42), (41), (38), (21), (20), (11), (11), (11), (4), (4), (1), (1), (1), (1), (1), (1), (1)
Pubmed 10 references available
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Product Details anti-CR1 Antibody

Target Details CR1 Application Details Handling References for anti-CR1 Antibody (ABIN2689207) Images
Brand BD Pharmingen™
Characteristics The 8C12 antibody recognizes an epitope present on the 190- kDa complement receptor protein, originally designated CR1 (CD35), but not the 145-150- kDa CR2 (CD21) molecule. Unlike the human system, in which these proteins are products of independent genes, both of these mouse receptors are membrane proteins resulting from the alternative splicing of mRNA transcribed from the Cr2 gene. Therefore, an alternative nomenclature has been proposed, designating the proteins Cr2-190 (CD21b) and Cr2-145 (CD21a), respectively. The epitope recognized by 8C12 mAb is only present on CD35/CD21b. Moreover, it has also been proposed that Crry is the true mouse genetic homologue of human CR1 (CD35). In the mouse, CD35 is expressed on the majority of peripheral B cells, on the majority of resident peritoneal macrophages, on peripheral blood granulocytes after treatment with N-formyl-Met-Leu-Phe, and on follicular dendritic cells, but not on thymocytes, T cells, erythrocytes, or platelets. In addition, it has not been detected, at the protein or mRNA level, in the macrophage cell line J774, bone marrow-derived macrophages, or thioglycollate-elicited peritoneal macrophages. The 8C12 mAb has been reported to inhibit rosette formation by C3bbearing sheep erythrocytes, to block the complement-dependent trapping of immune complexes by follicular dendritic cells, and to down-regulate mouse CD35 expression upon in vivo application, inhibiting only some primary antibody responses to immunization. B lymphocytes of Cr2[null] mice display impaired humoral immune responses in vivo. The 8C12 mAb recognizes an epitope on mouse CD35 distinct from the epitope recognized by anti-mouse CD21/CD35 mAb 7G6 (Cat. no. 553817), and it does not block binding by 7G6 mAb to CD35. *Please note that the isotype of 8C12 mAb was originally reported to be Rat IgG2c. Further investigations have demonstrated that the isotype of 8C12 mAb is Rat IgG2a.

BD Pharmingen™ Biotin Rat Anti-Mouse CD35 - Biotin - Clone 8C12 - Isotype Rat IgG2a, κ - Reactivity Ms - 0.5 mg
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Purified mouse CR1
Clone 8C12
Isotype IgG2a, kappa

Target Details CR1

Product Details anti-CR1 Antibody Application Details Handling References for anti-CR1 Antibody (ABIN2689207) Images back to top
Antigen
Alternative Name CD35 (CR1 Antibody Abstract)
Background Synonyms: CR1, CD21b
Research Area CD Antigens, Surface Receptors of Immune Cells
Pathways Complement System

Application Details

Product Details anti-CR1 Antibody Target Details CR1 Handling References for anti-CR1 Antibody (ABIN2689207) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-CR1 Antibody Target Details CR1 Application Details References for anti-CR1 Antibody (ABIN2689207) Images back to top
Concentration 0.5 mg/mL
Buffer Aqueous buffered solution containing ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.
Storage 4 °C
Storage Comment Store undiluted at 4°C.

References for anti-CR1 Antibody (ABIN2689207)

Product Details anti-CR1 Antibody Target Details CR1 Application Details Handling Images back to top
Product cited in:

Fischer, Goerg, Shen, Prodeus, Goodnow, Kelsoe, Carroll: "Dependence of germinal center B cells on expression of CD21/CD35 for survival." in: Science (New York, N.Y.), Vol. 280, Issue 5363, pp. 582-5, 1998

Fu, Huang, Wang, Chaplin: "B lymphocytes induce the formation of follicular dendritic cell clusters in a lymphotoxin alpha-dependent fashion." in: The Journal of experimental medicine, Vol. 187, Issue 7, pp. 1009-18, 1998

Hu, Martin, Weis, Weis: "Expression of the murine CD21 gene is regulated by promoter and intronic sequences." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 158, Issue 10, pp. 4758-68, 1997

Ahearn, Fischer, Croix, Goerg, Ma, Xia, Zhou, Howard, Rothstein, Carroll: "Disruption of the Cr2 locus results in a reduction in B-1a cells and in an impaired B cell response to T-dependent antigen." in: Immunity, Vol. 4, Issue 3, pp. 251-62, 1996

Molina, Holers, Li, Fung, Mariathasan, Goellner, Strauss-Schoenberger, Karr, Chaplin: "Markedly impaired humoral immune response in mice deficient in complement receptors 1 and 2." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 93, Issue 8, pp. 3357-61, 1996

Yoshida, van den Berg, Dijkstra: "Two functionally different follicular dendritic cells in secondary lymphoid follicles of mouse spleen, as revealed by CR1/2 and FcR gamma II-mediated immune-complex trapping." in: Immunology, Vol. 80, Issue 1, pp. 34-9, 1993

Martin, Weis: "Murine macrophages lack expression of the Cr2-145 (CR2) and Cr2-190 (CR1) gene products." in: European journal of immunology, Vol. 23, Issue 11, pp. 3037-42, 1993

Kinoshita, Thyphronitis, Tsokos, Finkelman, Hong, Sakai, Inoue: "Characterization of murine complement receptor type 2 and its immunological cross-reactivity with type 1 receptor." in: International immunology, Vol. 2, Issue 7, pp. 651-9, 1991

Heyman, Wiersma, Kinoshita: "In vivo inhibition of the antibody response by a complement receptor-specific monoclonal antibody." in: The Journal of experimental medicine, Vol. 172, Issue 2, pp. 665-8, 1990

Kurtz, OToole, Christensen, Weis: "The murine complement receptor gene family. IV. Alternative splicing of Cr2 gene transcripts predicts two distinct gene products that share homologous domains with both human CR2 and CR1." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 144, Issue 9, pp. 3581-91, 1990