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Human FCN1 ELISA Kit

Recommended FCN1 Product (supplied by: Log in to see )

Antigen
Ficolin (Collagen/fibrinogen Domain Containing) 1 (FCN1) ELISA Kits
  • FCN1
  • Fcn1
  • FCN2
  • Fcna
  • FCNB
  • FCNM
Epitope
AA 30-326
Reactivity
Human
Alternatives
Kits with alternative reactivity to:
10
7
5
2
1
1
1
1
Methode Type
Sandwich ELISA
Detection Range
0.78-50 ng/mL
Minimum Detection Limit
0.78 ng/mL
Application
ELISA
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Catalog No. ABIN1889408
$ 369.00
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
0.32666028 ABIN415288 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Biological Fluids 0.15-10 ng/mL 0.15 ng/mL Log in to see
0.32666028 ABIN1114945 Sandwich ELISA Serum, Plasma, Biological Fluids 0.16-10 ng/mL 0.16 ng/mL Log in to see
0.32666028 ABIN1569751 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 0.156-10 ng/mL 0.156 ng/mL Log in to see
0.32666028 ABIN2708212 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma 780-50000 pg/mL 780 pg/mL Log in to see
0.0012121415 ABIN839042 Sandwich ELISA Serum, Plasma, Tissue Homogenate 18.75-1200 pg/mL 18.75 pg/mL Log in to see
0.0012121415 ABIN454154 Cell Culture Supernatant, Serum, Plasma, Biological Fluids 0.312-20 ng/mL 0.312 ng/mL Log in to see
0.0012121415 ABIN2684243 Sandwich ELISA Plasma (heparin), Cell Culture Supernatant, Serum 0.78-50 ng/mL 0.78 ng/mL Log in to see
0.0012121415 ABIN2533475 Sandwich ELISA Tissue Homogenate, Cell Lysate, Biological Fluids 0.15-10 ng/mL 0.15 ng/mL Log in to see
0.0012121415 ABIN2947541 0.156-10 ng/mL 0.156 ng/mL Log in to see
0.0012121415 ABIN2112425 Competition ELISA Log in to see

Similar FCN1 ELISA Kits

Application / Reactivity Human
ELISA 11 ELISA Kits

General

Antigen Ficolin (Collagen/fibrinogen Domain Containing) 1 (FCN1) ELISA Kits
Epitope AA 30-326
Reactivity Human
Kits with alternative reactivity to:
(10), (7), (5), (2), (1), (1), (1), (1)
Methode Type Sandwich ELISA
Detection Range 0.78-50 ng/mL
Minimum Detection Limit 0.78 ng/mL
Application ELISA
Supplier Log in to see

Product Details FCN1 ELISA Kit

Target details Application Details Handling Images
Purpose For quantitative detection of human Ficolin-1 in cell culture supernates, serum and plasma(heparin).
Brand PicoKine™
Sample Type Cell Culture Supernatant, Serum, Plasma (heparin)
Detection Method Colorimetric
Specificity Natural and recombinant human Ficolin-1
Cross-Reactivity (Details) There is no detectable cross-reactivity with other relevant proteins.
Sensitivity <20pg/mL
Components
  • 96-well plate precoated with anti- human Ficolin-1 antibod - 1
  • Lyophilized recombinant human Ficolin-1 standar - 50ng/tubex2
  • Biotinylated anti- human Ficolin-1 antibod - 130μl(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex (ABC - 130μl(dilution 1:100)
  • Sample diluent buffe - 30ml
  • Antibody diluent buffe - 12ml
  • ABC diluent buffe - 12ml
  • TMB color developing agen - 10ml
  • TMB stop solutio - 10ml
Material not included Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
Immunogen Expression system for standard: NSO
Immunogen sequence: A30-A326

Target details

Product Details FCN1 ELISA Kit Application Details Handling Images back to top
Antigen
Alternative Name FCN1 (FCN1 ELISA Kit Abstract)
Background Ficolin-1, and also commonly termed M-ficolin is a protein that in humans is encoded by the FCN1 gene. It is mapped to 9q34.3. The ficolin family of proteins are characterized by the presence of a leader peptide, a short N-terminal segment, followed by a collagen-like region, and a C-terminal fibrinogen-like domain. Ficolins have a crucial role in defense against pneumococcal infection through the lectin complement pathway. Ficolin 1 is predominantly expressed in the peripheral blood leukocytes, and has been postulated to function as a plasma protein with elastin-binding activity.
Gene ID 2219
UniProt O00602
Research Area Immunology
Pathways Complement System

Application Details

Product Details FCN1 ELISA Kit Target details Handling Images back to top
Application Notes Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
Plate Pre-coated,Strips (12 x 8)
Protocol human Ficolin-1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Ficolin-1 has been precoated onto 96-well plates. Standards(NSO, A30-A326) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Ficolin-1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Ficolin-1 amount of sample captured in plate.
Assay Procedure Aliquot 0.1 mL per well of the 50 ng/mL, 25 ng/mL, 12.5 ng/mL, 6.25 ng/mL, 3.12 ng/mL, 1.56 ng/mL, 0.78 ng/mL human Ficolin-1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human Ficolin-1 standard solution and each sample be measured in duplicate.
Assay Precision
  • Sample 1: n=16, Mean(ng/ml): 4.83, Standard deviation: 0.188, CV(%): 3.9
  • Sample 2: n=16, Mean(ng/ml): 13.2, Standard deviation: 0.581, CV(%): 4.4
  • Sample 3: n=16, Mean(ng/ml): 25.1, Standard deviation: 1.431, CV(%): 5.7,
  • Sample 1: n=24, Mean(ng/ml): 5.24, Standard deviation: 0.393, CV(%): 7.5
  • Sample 2: n=24, Mean(ng/ml): 12.9, Standard deviation: 0.890, CV(%): 6.9
  • Sample 3: n=24, Mean(ng/ml): 25.9, Standard deviation: 1.89, CV(%): 7.3
Restrictions For Research Use only

Handling

Product Details FCN1 ELISA Kit Target details Application Details Images back to top
Handling Advice Avoid multiple freeze-thaw cycles
Storage -20 °C,4 °C
Storage Comment Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Expiry Date 12 months

Images

Product Details FCN1 ELISA Kit Target details Application Details Handling back to top
Supplier Images
ELISA image for FCN1 ELISA Kit (Ficolin (Collagen/fibrinogen Domain Containing) 1) (ABIN1889408) Human Ficolin-1 PicoKine ELISA Kit standard curve