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|Application / Reactivity||Human|
|ELISA||12 ELISA Kits|
|Antigen||Kallikrein 10 (KLK10) ELISA Kits|
Kits with alternative reactivity to:
|Methode Type||Competition ELISA|
|Detection Range||617.28-50000 pg/mL|
|Minimum Detection Limit||617.28 pg/mL|
|13 references available|
|Supplier||Log in to see|
Product Details Kallikrein 10 ELISA KitTarget details Application Details Handling References for Kallikrein 10 Kit (ABIN415167) Images
|Purpose||The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of NES1 in Serum,Plasma,Biological Fluids|
|Sample Type||Serum, Plasma, Biological Fluids|
|Specificity||This assay has high sensitivity and excellent specificity for detection of Nesfatin 1 (NES1).|
|Cross-Reactivity (Details)||No significant cross-reactivity or interference between Nesfatin 1 (NES1) and analogues was observed.|
The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by subtracting two standard deviations - the mean optical density value of twenty zero standard replicates and calculating the corresponding concentration.
|Material not included||
Target detailsProduct Details Kallikrein 10 ELISA Kit Application Details Handling References for Kallikrein 10 Kit (ABIN415167) Images back to top
|Alternative Name||NES1 (KLK10 ELISA Kit Abstract)|
Application DetailsProduct Details Kallikrein 10 ELISA Kit Target details Handling References for Kallikrein 10 Kit (ABIN415167) Images back to top
Information on standard material:
|Sample Volume||50 μL|
|Assay Time||2.5 h|
|Plate||Pre-coated,Strips (12 x 8)|
This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific for human NES1 has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled human NES1 and unlabeled human NES1 (Standards or samples) with the pre-coated antibody specific for human NES1. After incubation the unbound conjugate is washed off.
Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of NES1 in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of NES1 in the sample.
Serum: Allow samples to clot for two hours at room temperature or overnight at 4°C before centrifugation for 20 minutes at approximately 1000 × g. Assay immediately or store samples in aliquot at -20°C or -80°C. Avoid repeated freeze/thaw cycles.
Plasma: Collect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples for 15 minutes at 1000 × g within 30 minutes of collection. Remove plasma and assay immediately or store samples in aliquot at -20°C or -80°C. Avoid repeated freeze/thaw cycles.
Biological Fluids: Centrifuge samples for 20 minutes at 1000 × g. Remove particulates and assay immediately or store samples in aliquot at -20 °C or -80 °C for later use. Avoid repeated freeze/thaw cycles.
|Calculation of Results||
This assay employs the competitive inhibition enzyme immunoassay technique, so there is an inverse correlation between NES1 concentration in the sample and the assay signal intensity. Average the duplicate readings for each standard, control, and samples. Create a standard curve on log-log or semi-log graph paper, with the log of NES1 concentration on the y-axis and absorbance on the x-axis. Draw the best fit straight line through the standard points and it can be determined by regression analysis. Using some plot software, for instance, curve expert 1.30, is also recommended. If samples have been diluted, the concentration read from the standard curve must be multiplied by the dilution factor.
In order to make the calculation easier, we plot the O.D. value of the standard (X-axis) against the log of concentration of the standard (Y-axis), although concentration is the independent variable and O.D. value is the dependent variable. The O.D. values of the standard curve may vary according to the conditions of assay performance (e.g. operator, pipetting technique, washing technique or temperature effects). Typical standard curve below is provided for reference only.
Intra-assay Precision (precision within an assay): 3 samples with low, middle and high level human NES1 were tested 20 times on one plate, respectively.
Inter-assay Precision (precision between assays): 3 samples with low, middle and high level human NES1 were tested on 3 different plates, 8 replicates in each plate.
CV (%) = SD/mean X 100
|Restrictions||For Research Use only|
HandlingProduct Details Kallikrein 10 ELISA Kit Target details Application Details References for Kallikrein 10 Kit (ABIN415167) Images back to top
|Precaution of Use||The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.|
|Handling Advice||To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.|
|Storage||4 °C/-20 °C|
|Expiry Date||6 months|
References for Kallikrein 10 Kit (ABIN415167)Product Details Kallikrein 10 ELISA Kit Target details Application Details Handling Images back to top
|Product cited in:||
Ustabaş Kahraman, Vehapoğlu, Özgen, Terzioğlu, Cesur, Dündaröz: "Correlation of Brain Neuropeptide (Nesfatin-1 and Orexin-A) Concentrations with Anthropometric and Biochemical Parameters in Malnourished Children." in: Journal of clinical research in pediatric endocrinology, Vol. 7, Issue 3, pp. 197-202, 2016
Ahmadizad, Avansar, Ebrahim, Avandi, Ghasemikaram: "The effects of short-term high-intensity interval training vs. moderate-intensity continuous training on plasma levels of nesfatin-1 and inflammatory markers." in: Hormone molecular biology and clinical investigation, Vol. 21, Issue 3, pp. 165-73, 2015
Ülger, Gül, Uslukaya, O?uz, Bozda?, Yüksel, Böyük: "New hormones to predict the severity of gallstone-induced acute pancreatitis." in: The Turkish journal of gastroenterology : the official journal of Turkish Society of Gastroenterology, Vol. 25, Issue 6, pp. 714-7, 2015
Alp, Görmüş, Güdücü, Bozkurt: "Nesfatin-1 levels and metabolic markers in polycystic ovary syndrome." in: Gynecological endocrinology : the official journal of the International Society of Gynecological Endocrinology, Vol. 31, Issue 7, pp. 543-7, 2015
Araz, Yilmazel Ucar, Dorman, Bayraktutan, Yayla, Yilmaz, Acemoglu, Halici, Akgun: "Is There a Relationship between Obstructive Sleep Apnea Syndrome Severity and Nesfatin-1?" in: Respiration; international review of thoracic diseases, Vol. 90, Issue 2, pp. 105-10, 2015
Sahin, Sahin, Ural, Cure, Senturk, Tekin, Balik, Cure, Yuce, Kirbas: "Nesfatin-1 and Vitamin D levels may be associated with systolic and diastolic blood pressure values and hearth rate in polycystic ovary syndrome." in: Bosnian journal of basic medical sciences / Udruženje basičnih mediciniskih znanosti = Association of Basic Medical Sciences, Vol. 15, Issue 3, pp. 57-63, 2015
?engül, Dilbaz, Hal?c?, Ferah, Çad?rc?, Y?lmaz: "Decreased serum nesfatin-1 levels in endometriosis." in: European journal of obstetrics, gynecology, and reproductive biology, Vol. 177, pp. 34-7, 2014
Ademoglu, Gorar, Carl?oglu, Yaz?c?, Dellal, Berberoglu, Akdeniz, Uysal, Karakurt: "Plasma nesfatin-1 levels are increased in patients with polycystic ovary syndrome." in: Journal of endocrinological investigation, Vol. 37, Issue 8, pp. 715-9, 2014
Türko?lu, Böyük, Tanr?verdi, Gündüz, Dusak, Kaplan, Gümü?: "The potential role of BMI, plasma leptin, nesfatin-1 and ghrelin levels in the early detection of pancreatic necrosis and severe acute pancreatitis: a prospective cohort study." in: International journal of surgery (London, England), Vol. 12, Issue 12, pp. 1310-3, 2014
Aslan, Celik, Celik, Turkcuoglu, Yilmaz, Karaer, Simsek, Celik, Aydin: "Cord blood nesfatin-1 and apelin-36 levels in gestational diabetes mellitus." in: Endocrine, Vol. 41, Issue 3, pp. 424-9, 2012
Gunay, Tutuncu, Aydin, Dag, Abasli: "Decreased plasma nesfatin-1 levels in patients with generalized anxiety disorder." in: Psychoneuroendocrinology, Vol. 37, Issue 12, pp. 1949-53, 2012
Ari, Ozturk, Bez, Oktar, Erduran: "High plasma nesfatin-1 level in patients with major depressive disorder." in: Progress in neuro-psychopharmacology & biological psychiatry, Vol. 35, Issue 2, pp. 497-500, 2011
Johnson, Drugan, Miller, Evans: "38" in: , Vol. 1363, Issue Nucleic acids research, pp. 28-39, 1991
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