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|Application / Reactivity||Rat (Rattus)||Mouse (Murine)|
|ELISA||2 Antibodies||5 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||13 Antibodies||13 Antibodies|
|Immunohistochemistry (IHC)||3 Antibodies||7 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||6 Antibodies||6 Antibodies|
|Western Blotting (WB)||11 Antibodies||18 Antibodies|
|Immunocytochemistry (ICC)||1 Antibodies|
|Immunofluorescence (fixed cells) (IF/ICC)||1 Antibodies|
|Immunoprecipitation (IP)||1 Antibodies|
|Antigen||Kallikrein 5 (KLK5) Antibodies|
|Epitope||AA 278-293, C-Term Alternatives|
|Reactivity||Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This Kallikrein 5 antibody is un-conjugated Alternatives|
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Product Details anti-Kallikrein 5 AntibodyTarget Details Kallikrein 5 Application Details Handling Images
|Cross-Reactivity (Details)||No cross reactivity with other proteins.|
Rabbit IgG polyclonal antibody for Kallikrein related-peptidase 5/Protein Klk5(KLK5) detection. Tested with WB, IHC-P in Mouse,Rat.
Gene Name: kallikrein-related peptidase 5
Protein Name: Kallikrein related-peptidase 5/Protein Klk5
|Purification||Immunogen affinity purified.|
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of mouse Kallikrein 5(278-293aa NLCEFVKWIKDTMNSN), different from the related rat sequence by three amino acids.|
Target Details Kallikrein 5Product Details anti-Kallikrein 5 Antibody Application Details Handling Images back to top
|Alternative Name||KLK5 (KLK5 Antibody Abstract)|
|Background||KLK5(Kallikrein-related peptidase 5), formerly known as stratum corneum tryptic enzyme(SCTE) or KLKL2, is a serine protease expressed in the epidermis. The KLK5 gene is one of the fifteen kallikrein subfamily members which is located on 19q13.41. KLK5 has been suggested to regulate cell shedding in conjunction with KLK7 and KLK14, given its ability to degrade proteins which form the extracellular component of cell junctions in the stratum corneum. The KLK5 gene contains 5 coding exons spanning 9.3. KLK5 expression is upregulated in a breast cancer cell line in the presence of estrogens and progestins and is associated with more aggressive forms of epithelial ovarian carcinoma.|
Application DetailsProduct Details anti-Kallikrein 5 Antibody Target Details Kallikrein 5 Handling Images back to top
WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Mouse
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Rat, Predicted Species: Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.
Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).
|Restrictions||For Research Use only|
HandlingProduct Details anti-Kallikrein 5 Antibody Target Details Kallikrein 5 Application Details Images back to top
|Reconstitution||Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.|
|Buffer||Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.|
|Preservative||Thimerosal (Merthiolate), Sodium azide|
|Precaution of Use||This product contains Thimerosal (Merthiolate),Sodium azide a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||-20 °C,4 °C|
At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
|Expiry Date||12 months|
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