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Human Kallikrein 6 ELISA Kit

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Antigen
Kallikrein 6 (KLK6) ELISA Kits
  • AI849898
  • BSP
  • Bssp
  • hK6
  • Klk1b21
  • klk6
  • Klk6
  • Klk7
  • KLK8
  • Klk29
  • MSP
  • neurosin
  • Prss9
  • PRSS9
  • PRSS18
  • Prss18
  • rGK-8
  • rk8
  • SP59
Epitope
AA 22-244
Reactivity
Human
Alternatives
Kits with alternative reactivity to:
16
9
7
3
3
3
3
2
2
1
1
1
Methode Type
Sandwich ELISA
Detection Range
78-5000 pg/mL
Minimum Detection Limit
78 pg/mL
Application
ELISA
Supplier
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Catalog No. ABIN1889322
$ 369.00
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
0.32693866 ABIN414604 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cerebrospinal Fluid, Milk, Biological Fluids 0.156-10 ng/mL 0.156 ng/mL Log in to see
0.32693866 ABIN1115703 Sandwich ELISA Serum, Plasma, Biological Fluids 0.31-20 ng/mL 0.31 ng/mL Log in to see
0.32693866 ABIN1029060 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 0.313-20 ng/mL 0.313 ng/mL Log in to see
0.32693866 ABIN2708302 Sandwich ELISA Cell Culture Supernatant, Cell Lysate, Serum, Plasma, Tissue Homogenate 78-5000 pg/mL 78 pg/mL Log in to see
0.0012117105 ABIN1873375 Competition ELISA Serum, Plasma, Tissue Homogenate, Cerebrospinal Fluid, Milk, Biological Fluids 123.46-10000 pg/mL 123.46 pg/mL Log in to see
0.0012117105 ABIN367015 Sandwich ELISA 1.25-80 ng/mL 1.25 ng/mL Log in to see
0.0012117105 ABIN626518 Competition ELISA Serum, Cell Culture Supernatant, Tissue Homogenate, Plasma, Body Fluids 1.0-25 ng/mL 1 ng/mL Log in to see
0.0012117105 ABIN455645 Cell Culture Supernatant, Serum, Plasma, Tissue Homogenate, Biological Fluids 0.312-20 ng/mL 0.312 ng/mL Log in to see
0.0012117105 ABIN2703283 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum, Urine 0.6-150 ng/mL 0.6 ng/mL Log in to see
0.0012117105 ABIN1110860 Sandwich ELISA Log in to see 1
0.0012117105 ABIN2684808 Sandwich ELISA Plasma (heparin), Plasma (EDTA), Cell Culture Supernatant, Serum, Tissue Homogenate 78-5000 pg/mL 78 pg/mL Log in to see
0.0012117105 ABIN2043843 Competition ELISA Serum, Plasma, Tissue Homogenate, Cerebrospinal Fluid, Milk, Biological Fluids 123.5-10000 pg/mL 123.5 pg/mL Log in to see
0.0012117105 ABIN2535765 Competition ELISA Serum, Plasma, Tissue Homogenate, Cerebrospinal Fluid, Biological Fluids 123.46-10000 pg/mL 123.46 pg/mL Log in to see
0.0012117105 ABIN2089425 Sandwich ELISA Log in to see
0.0012117105 ABIN2948958 0.313-20 ng/mL 0.313 ng/mL Log in to see
0.0012117105 ABIN2112647 Competition ELISA Log in to see

Top referenced Kallikrein 6 ELISA Kit for ELISA

Similar Kallikrein 6 ELISA Kits

Application / Reactivity Human
ELISA 17 ELISA Kits

General

Antigen Kallikrein 6 (KLK6) ELISA Kits
Epitope AA 22-244
Reactivity Human
Kits with alternative reactivity to:
(16), (9), (7), (3), (3), (3), (3), (2), (2), (1), (1), (1)
Methode Type Sandwich ELISA
Detection Range 78-5000 pg/mL
Minimum Detection Limit 78 pg/mL
Application ELISA
Pubmed 2 references available
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Product Details Kallikrein 6 ELISA Kit

Target details Application Details Handling References for Kallikrein 6 Kit (ABIN1889322) Images
Purpose For quantitative detection of human Kallikrein-6 in cell culture supernates, cell lysates, serum, plasma(heparin, EDTA) and tissue homogenates.
Brand PicoKine™
Sample Type Cell Culture Supernatant, Cell Lysate, Serum, Plasma (heparin), Plasma (EDTA), Tissue Homogenate
Detection Method Colorimetric
Specificity Natural and recombinant human Kallikrein-6
Cross-Reactivity (Details) There is no detectable cross-reactivity with other relevant proteins.
Sensitivity <3pg/mL
Components
  • 96-well plate precoated with anti- human Kallikrein-6 antibod - 1
  • Lyophilized recombinant human Kallikrein-6 standar - 10ng/tubex2
  • Biotinylated anti- human Kallikrein-6 antibod - 130μl(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex (ABC - 130μl(dilution 1:100)
  • Sample diluent buffe - 30 ml
  • Antibody diluent buffe - 12ml
  • ABC diluent buffe - 12ml
  • TMB color developing agen - 10ml
  • TMB stop solutio - 10ml
Material not included Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
Immunogen Expression system for standard: NSO
Immunogen sequence: L22-K244

Target details

Product Details Kallikrein 6 ELISA Kit Application Details Handling References for Kallikrein 6 Kit (ABIN1889322) Images back to top
Antigen
Alternative Name KLK6 (KLK6 ELISA Kit Abstract)
Background KLK6(Kallikrein-related peptidase 6), also called KALLIKREIN 6, NEUROSIN, PROTEASE M, ZYME or PRSS9, is a protein that in humans is encoded by the KLK6 gene. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. The encoded enzyme is regulated by steroid hormones. Northern blot analysis revealed that the PRSS9 mRNA was expressed in several primary tumors and cell lines from mammary, prostate, and ovarian cancers, but was not detected in any metastases of these cancers. The KLK6 gene is mapped on 19q13.41. In tissue culture, the enzyme has been found to generate amyloidogenic fragments from the amyloid precursor protein, suggesting a potential for involvement in Alzheimer's disease. Upon cellular stress, neurosin was released from mitochondria to the cytosol, which resulted in the increase of degraded alpha-synuclein species. Neurosin may play a significant role in physiologic alpha-synuclein degradation and also in the pathogenesis of synucleinopathies.
Gene ID 5653
UniProt Q92876
Pathways Complement System

Application Details

Product Details Kallikrein 6 ELISA Kit Target details Handling References for Kallikrein 6 Kit (ABIN1889322) Images back to top
Application Notes Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
Plate Pre-coated,Strips (12 x 8)
Protocol human Kallikrein-6 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Kallikrein-6 has been precoated onto 96-well plates. Standards(NSO, L22-K244) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Kallikrein-6 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Kallikrein-6 amount of sample captured in plate.
Assay Procedure Aliquot 0.1 mL per well of the 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL, 78pg/mL human Kallikrein-6 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, cell lysates, serum, plasma(heparin, EDTA) or tissue homogenates to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each human Kallikrein-6 standard solution and each sample is measured in duplicate.
Assay Precision
  • Sample 1: n=16, Mean(pg/ml): 545, Standard deviation: 37.61, CV(%): 6.9
  • Sample 2: n=16, Mean(pg/ml): 1711, Standard deviation: 80.42, CV(%): 4.7
  • Sample 3: n=16, Mean(pg/ml): 2903, Standard deviation: 168.4, CV(%): 5.8,
  • Sample 1: n=24, Mean(pg/ml): 721, Standard deviation: 55.52, CV(%): 7.7
  • Sample 2: n=24, Mean(pg/ml): 1927, Standard deviation: 104.1, CV(%): 5.4
  • Sample 3: n=24, Mean(pg/ml): 3120, Standard deviation: 196.6, CV(%): 6.3
Restrictions For Research Use only

Handling

Product Details Kallikrein 6 ELISA Kit Target details Application Details References for Kallikrein 6 Kit (ABIN1889322) Images back to top
Handling Advice Avoid multiple freeze-thaw cycles
Storage -20 °C,4 °C
Storage Comment Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Expiry Date 12 months

References for Kallikrein 6 Kit (ABIN1889322)

Product Details Kallikrein 6 ELISA Kit Target details Application Details Handling Images back to top
Background publications

Elliott, Irwin, Diamandis: "In silico identification and Bayesian phylogenetic analysis of multiple new mammalian kallikrein gene families." in: Genomics, Vol. 88, Issue 5, pp. 591-9, 2006

Anisowicz, Sotiropoulou, Stenman, Mok, Sager: "A novel protease homolog differentially expressed in breast and ovarian cancer." in: Molecular medicine (Cambridge, Mass.), Vol. 2, Issue 5, pp. 624-36, 1997

Images

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Supplier Images
ELISA image for Kallikrein 6 ELISA Kit (KLK6) (ABIN1889322) Human Kallikrein-6 PicoKine ELISA Kit standard curve