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anti-Pig (Porcine) KLKB1 antibody for Western Blotting

Recommended KLKB1 Antibody (supplied by: Log in to see )

Antigen
Kallikrein B, Plasma (Fletcher Factor) 1 (KLKB1) Antibodies
  • APS
  • DKFZp459J2015
  • Kal-3
  • Kal3
  • Kalp1
  • KALP15
  • KLK3
  • Klk3
  • KLKB1
  • PPK
  • PSA
Epitope
Middle Region
22
19
9
9
3
3
2
2
1
1
Reactivity
Human, Pig (Porcine)
77
23
6
Host
Rabbit
62
22
5
1
1
Clonality
Polyclonal
Conjugate
This KLKB1 antibody is un-conjugated
6
6
6
3
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Western Blotting (WB)
67
59
11
10
6
2
2
1
1
Supplier
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Catalog No. ABIN2782312
$ 229.00
Plus shipping costs $45.00

Top referenced anti-KLKB1 antibody for Western Blotting

  • Human Polyclonal KLKB1 Primary Antibody for WB - ABIN2782312 (1 Publications): Tang, Yu, Williams, Springman, Jeffery, Sprengeler, Estevez, Sampang, Shrader, Spencer, Young, McGrath, Katz: Expression, crystallization, and three-dimensional structure of the catalytic domain of human plasma kallikrein. in The Journal of biological chemistry 2005 (PubMed)

Similar anti-KLKB1 Antibodies

Application / Reactivity Human Pig (Porcine)
Western Blotting (WB) 61 Antibodies 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 10 Antibodies
Immunohistochemistry (IHC) 7 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 1 Antibodies
Immunofluorescence (IF) 6 Antibodies
Immunoelectrophoresis (IEP) 2 Antibodies
Immunodiffusion (ID) 1 Antibodies
Immunocytochemistry (ICC) 2 Antibodies
ELISA 55 Antibodies

General

Antigen Kallikrein B, Plasma (Fletcher Factor) 1 (KLKB1) Antibodies
Epitope Middle Region
(22), (19), (9), (9), (3), (3), (2), (2), (1), (1)
Reactivity Human, Pig (Porcine)
(77), (23), (6)
Host Rabbit
(62), (22), (5), (1), (1)
Clonality
Polyclonal
Conjugate This KLKB1 antibody is un-conjugated
(6), (6), (6), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Western Blotting (WB)
(67), (59), (11), (10), (6), (2), (2), (1), (1)
Pubmed 1 reference available
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Product Details anti-KLKB1 Antibody

Target Details KLKB1 Application Details Handling References for anti-KLKB1 Antibody (ABIN2782312) Images
Sequence VLTAAHCFDG LPLQDVWRIY SGILNLSDIT KDTPFSQIKE IIIHQNYKVS
Predicted Reactivity Human : 100 %, Dog : 80 %, Horse : 80 %, Pig : 70 %
Characteristics This is a rabbit polyclonal antibody against KLKB1. It was validated on Western Blot using a cell lysate as a positive control.
Purification Protein A purified
Immunogen The immunogen is a synthetic peptide directed towards the middle region of human KLKB1

Target Details KLKB1

Product Details anti-KLKB1 Antibody Application Details Handling References for anti-KLKB1 Antibody (ABIN2782312) Images back to top
Antigen
Alternative Name KLKB1 (KLKB1 Antibody Abstract)
Background Plasma prekallikrein is a glycoprotein that participates in the surface-dependent activation of blood coagulation, fibrinolysis, kinin generation and inflammation. It is synthesized in the liver and secreted into the blood as a single polypeptide chain. Plasma prekallikrein is converted to plasma kallikrein by factor XIIa by the cleavage of an internal Arg-Ile bond. Plasma prekallikrein deficiency causes a prolonged activated partial thromboplastin time in patients.Plasma prekallikrein is a glycoprotein that participates in the surface-dependent activation of blood coagulation, fibrinolysis, kinin generation and inflammation. It is synthesized in the liver and secreted into the blood as a single polypeptide chain. Plasma prekallikrein is converted to plasma kallikrein by factor XIIa by the cleavage of an internal Arg-Ile bond. Plasma kallikrein therefore is composed of a heavy chain and a light chain held together by a disulphide bond. The heavy chain originates from the amino-terminal end of the zymogen and contains 4 tandem repeats of 90 or 91 amino acids. Each repeat harbors a novel structure called the apple domain. The heavy chain is required for the surface-dependent pro-coagulant activity of plasma kallikrein. The light chain contains the active site or catalytic domain of the enzyme and is homologous to the trypsin family of serine proteases. Plasma prekallikrein deficiency causes a prolonged activated partial thromboplastin time in patients.
Molecular Weight 28 kDa
Gene ID 3818
NCBI Accession NP_000883, NM_000892
UniProt P03952
Pathways Complement System

Application Details

Product Details anti-KLKB1 Antibody Target Details KLKB1 Handling References for anti-KLKB1 Antibody (ABIN2782312) Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 638 AA

Restrictions For Research Use only

Handling

Product Details anti-KLKB1 Antibody Target Details KLKB1 Application Details References for anti-KLKB1 Antibody (ABIN2782312) Images back to top
Format Liquid
Reconstitution Add 100 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage 4 °C,-20 °C
Storage Comment For short term use, store at 2-8 °C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-KLKB1 Antibody (ABIN2782312)

Product Details anti-KLKB1 Antibody Target Details KLKB1 Application Details Handling Images back to top
Product cited in:

Tang, Yu, Williams, Springman, Jeffery, Sprengeler, Estevez, Sampang, Shrader, Spencer, Young, McGrath, Katz: "Expression, crystallization, and three-dimensional structure of the catalytic domain of human plasma kallikrein." in: The Journal of biological chemistry, Vol. 280, Issue 49, pp. 41077-89, 2005

Images

Product Details anti-KLKB1 Antibody Target Details KLKB1 Application Details Handling References for anti-KLKB1 Antibody (ABIN2782312) back to top
Supplier Images
Western Blotting (WB) image for anti-KLKB1 antibody (Kallikrein B, Plasma (Fletcher Factor) 1) (Middle Region) (ABIN2782312) anti-Kallikrein B, Plasma (Fletcher Factor) 1 (KLKB1) (Middle Region) antibody