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Human MBL2 ELISA Kit

Recommended MBL2 Product (supplied by: Log in to see )

Antigen
Mannose-Binding Lectin (Protein C) 2, Soluble (MBL2) ELISA Kits
  • Ab2-001
  • Ab2-011
  • cMBl
  • COLEC1
  • collectin
  • HSMBPC
  • L-MBP
  • MBL
  • MBL-C
  • MBL1
  • mbl2
  • MBL2D
  • MBP
  • MBP-C
  • MBP1
  • MBPD
  • pMBP-27
Epitope
AA 21-248
Reactivity
Human
Alternatives
Kits with alternative reactivity to:
8
8
5
3
2
1
1
1
1
1
1
1
Methode Type
Sandwich ELISA
Detection Range
312-20000 pg/mL
Minimum Detection Limit
312 pg/mL
Application
ELISA
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Catalog No. ABIN2859302
$ 369.00
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
0.0012117105 ABIN1873266 Sandwich ELISA Serum, Plasma, Biological Fluids 6.25-400 ng/mL 6.25 ng/mL Log in to see
0.0012117105 ABIN455817 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma 0.156-10 ng/mL 0.156 ng/mL Log in to see
0.0012117105 ABIN2684937 Sandwich ELISA Cell Lysate, Plasma, Serum Log in to see
0.0012117105 ABIN2536366 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 0.156-10 ng/mL 0.156 ng/mL Log in to see
0.0012117105 ABIN2091133 Sandwich ELISA Log in to see

Similar MBL2 ELISA Kits

Application / Reactivity Human
ELISA 6 ELISA Kits

General

Antigen Mannose-Binding Lectin (Protein C) 2, Soluble (MBL2) ELISA Kits
Epitope AA 21-248
Reactivity Human
Kits with alternative reactivity to:
(8), (8), (5), (3), (2), (1), (1), (1), (1), (1), (1), (1)
Methode Type Sandwich ELISA
Detection Range 312-20000 pg/mL
Minimum Detection Limit 312 pg/mL
Application ELISA
Supplier Log in to see

Product Details MBL2 ELISA Kit

Target details Application Details Handling Images
Purpose For quantitative detection of human MBP-C in cell culture supernates, serum and plasma(heparin, EDTA).
Brand PicoKine™
Sample Type Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
Detection Method Colorimetric
Specificity Natural and recombinant human MBP-C
Cross-Reactivity (Details) There is no detectable cross-reactivity with other relevant proteins.
Sensitivity <10pg/mL
Components
  • 96-well plate precoated with anti- human MBP-C antibod - 1
  • Lyophilized recombinant human MBP-C standar - 20ng/tubex2
  • Biotinylated anti- human MBP-C antibod - 130μl(dilution 1:100)
  • Avidin-Biotin-Peroxidase Complex (ABC - 130μl(dilution 1:100)
  • Sample diluent buffe - 30 ml
  • Antibody diluent buffe - 12ml
  • ABC diluent buffe - 12ml
  • TMB color developing agen - 10ml
  • TMB stop solutio - 10ml
Immunogen Expression system for standard: NSO
Immunogen sequence: E21-I248

Target details

Product Details MBL2 ELISA Kit Application Details Handling Images back to top
Antigen
Alternative Name MBL2 (MBL2 ELISA Kit Abstract)
Background MBL2, also called mannose-binding lectin (protein C) 2, soluble or Mannose-binding lectin (MBL) is a lectin that is instrumental in innate immunity. MBL2 is mapped to chromosome 10q11.2-q21. It belongs to the class of collectins in the C-type lectin superfamily, whose function appears to be pattern recognition in the first line of defense in the pre-immune host. MBL2 recognizes carbohydrate patterns, found on the surface of a large number of pathogenic micro-organisms, including bacteria, viruses, protozoa and fungi. Binding MBL2 to a micro-organism results in activation of the lectin pathway of the complement system. Another important function of MBL2 is that this molecule binds senescent and apoptotic cells and enhances engulfment of whole, intact apoptotic cells, as well as cell debris by phagocytes.
Gene ID 4153
UniProt P11226
Pathways Complement System

Application Details

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Application Notes Optimal working dilution should be determined by the investigator.
Plate Pre-coated,Strips (12 x 8)
Protocol human MBP-C ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for MBP-C has been precoated onto 96-well plates. Standards(NSO, E21-I248) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for MBP-C is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human MBP-C amount of sample captured in plate.
Assay Procedure Aliquot 0.1 mL per well of the 20000pg/mL, 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL human MBP-C standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human MBP-C standard solution and each sample be measured in duplicate.
Assay Precision
  • Sample 1: n=16, Mean(ng/ml): 2.4, Standard deviation: 0.086, CV(%): 3.6
  • Sample 2: n=16, Mean(ng/ml): 7.2, Standard deviation: 0.324, CV(%): 4.5
  • Sample 3: n=16, Mean(ng/ml): 11.5, Standard deviation: 0.61, CV(%): 5.3,
  • Sample 1: n=24, Mean(ng/ml): 3.2, Standard deviation: 0.157, CV(%): 4.9
  • Sample 2: n=24, Mean(ng/ml): 6.9, Standard deviation: 0.393, CV(%): 5.7
  • Sample 3: n=24, Mean(ng/ml): 10.7, Standard deviation: 0.738, CV(%): 6.9
Restrictions For Research Use only

Handling

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Storage -20 °C,4 °C
Storage Comment Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Expiry Date 12 months

Images

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Supplier Images
ELISA image for MBL2 ELISA Kit (Mannose-Binding Lectin (Protein C) 2, Soluble) (ABIN2859302) Human MBP-C/MBL2 PicoKine ELISA Kit standard curve