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Thrombospondin 1 antibody (Collagen-binding Domain)

Name: Thrombospondin 1 antibody (Collagen-binding Domain)
SKU: ABIN337292
Price: 727.83 USD
Availability: InStock

THBS1 Reactivity: Human, Mouse, Rat, Cow, Pig, Horse, Sheep, Dog WB, IHC, IP, IF, FACS, IHC (p) Host: Mouse Monoclonal A6-1 unconjugated

Anti-Thrombospondin antibody IHC of human skeletal muscle.

IHC

Human Skeletal Muscle (formalin-fixed, paraffin-embedded) stained with THBS1 antibody ABIN337292 at 10 ug/ml followed by biotinylated anti-mouse IgG secondary antibody ABIN481714, alkaline phosphatase-streptavidin and chromogen.

IHC (p)

2 images

Catalog No. ABIN337292

Datasheet as PDF

Target See all Thrombospondin 1 (THBS1) Antibodies

Thrombospondin 1 (THBS1)
Binding Specificity
10

4

3

3

3

3

3

2

1

1

1

1

1

1

1

1

1

1

1

1
Collagen-binding Domain
Reactivity
47

25

18

4

1

1

1

1

1

1
Human, Mouse, Rat, Cow, Pig, Horse, Sheep, Dog
Host
69

6

2
Mouse
Compatible Secondaries
Clonality
70

7
Monoclonal
Conjugate
38

17

9

4

1

1

1

1

1

1

1

1

1
This Thrombospondin 1 antibody is un-conjugated
Application
49

30

27

15

10

7

7

3

3

2

2

2

2

2

2

2

1

1

1
Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand

IHC-plus™

Specificity

Recognizes Thrombospondin. Shows no cross-reactivity with fibronectin, fibrinogen and von Willebrand factor. Shows a mild cross-reaction with TSP 2. Inhibits TSP-collagen interaction. Antibody binding to TSP is unaffected by glycosaminoglycans (e. g. hyaluronic acid, chondroitin sulfate and heparin). Its binding is enhanced by EDTA i. e. at low concentration of Ca2+. Species cross-reactivity: Human, bovine, porcine, equine, canine, sheep, mouse and rat.

Purification

Protein G purified

Immunogen

Reduced and alkylated purified human TSP (fully denatured) from the supernatant of thrombin-activated platelets. MW of Antigen: ~450kD (non-reduced), 170-180kD (reduced). Epitope: Collagen Type V-binding domain of TSP.

Clone

A6-1

Isotype

IgG1

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THBS1 Reactivity: Human WB, IF Host: Mouse Polyclonal unconjugated

anti-Thrombospondin 1 (THBS1) (Internal Region) antibody
THBS1 Reactivity: Human, Mouse, Rat WB, ELISA, IHC, ICC, IF Host: Rabbit Polyclonal unconjugated

Application Notes

Approved: Flo, IF, IHC, IHC-P (10 μg/mL), IP, WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL. The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.

Comment

Target Species of Antibody: Human

Restrictions

For Research Use only
Format

Liquid

Concentration

Lot specific

Buffer

PBS,  pH 7.4.

Preservative

Azide free

Handling Advice

Avoid repeat freeze-thaw cycles.

Storage

4 °C,-20 °C

Storage Comment

Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
Target

Thrombospondin 1 (THBS1)

Alternative Name

Thrombospondin 1 (THBS1 Products)

Synonyms

THBS antibody, THBS-1 antibody, TSP antibody, TSP-1 antibody, TSP1 antibody, Thbs-1 antibody, tbsp1 antibody, thrombospondin-1 antibody, thbs antibody, thbs-1 antibody, tsp antibody, tsp-1 antibody, tsp1 antibody, Tsp1 antibody, THBS1 antibody, Tsp-1 antibody, wu:fc48c03 antibody, si:dkey-11e23.1 antibody, tsp2 antibody, thrombospondin 1 antibody, thrombospondin 1 S homeolog antibody, thrombospondin 1b antibody, thrombospondin 2 L homeolog antibody, THBS1 antibody, Thbs1 antibody, thbs1.S antibody, thbs1b antibody, thbs1 antibody, thbs2.L antibody

Pathways

Autophagy