CXCR7 antibody (Extracellular Domain)
-
- Target See all CXCR7 Antibodies
- CXCR7 (Chemokine (C-X-C Motif) Receptor 7 (CXCR7))
-
Binding Specificity
- Extracellular Domain
-
Reactivity
- Human, Mouse, Rat, Dog, Cow, Pig, Horse, Hamster, Rabbit, Bat
-
Host
- Rabbit
-
Clonality
- Polyclonal
-
Conjugate
- This CXCR7 antibody is un-conjugated
-
Application
- Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Brand
- IHC-plus™
- Specificity
- Human CXCR7. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
- Predicted Reactivity
- Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Mouse, Rat, Bovine, Bat, Dog, Hamster, Panda, Horse, Rabbit, Pig (100%) Marmoset, Elephant (95%) Opossum, Platypus (84%).
- Purification
- Immunoaffinity purified
- Immunogen
-
Synthetic 19 amino acid peptide from 2nd extracellular domain of human CXCR7. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Mouse, Rat, Bovine, Bat, Dog, Hamster, Panda, Horse, Rabbit, Pig (100%), Marmoset, Elephant (95%), Opossum, Platypus (84%).
Type of Immunogen: Synthetic peptide - Top Product
- Discover our top product CXCR7 Primary Antibody
-
anti-Chemokine (C-X-C Motif) Receptor 7 (CXCR7) antibody
KO Validated CXCR7 Reactivity: Human WB, ELISA, FACS, IHC (p), IF, IHC (fro), IP, ICC Host: Rabbit Polyclonal unconjugated
anti-Chemokine (C-X-C Motif) Receptor 7 (CXCR7) (Internal Region) antibodyCXCR7 Reactivity: Human, Mouse, Rat WB, ELISA, IF, ICC Host: Rabbit Polyclonal unconjugated
anti-Chemokine (C-X-C Motif) Receptor 7 (CXCR7) antibodyCXCR7 Reactivity: Human, Mouse, Rat IHC Host: Rabbit Polyclonal unconjugated
anti-Chemokine (C-X-C Motif) Receptor 7 (CXCR7) (AA 151-250) antibodyCXCR7 Reactivity: Human, Rat WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro), ICC Host: Rabbit Polyclonal unconjugated
anti-Chemokine (C-X-C Motif) Receptor 7 (CXCR7) (AA 330-358), (C-Term) antibodyCXCR7 Reactivity: Human WB, IHC (p) Host: Rabbit Polyclonal RB23520 unconjugated
anti-Chemokine (C-X-C Motif) Receptor 7 (CXCR7) (C-Term) antibodyCXCR7 Reactivity: Human, Mouse, Rat WB, IHC (p), IF, EIA Host: Rabbit Polyclonal unconjugated
anti-Chemokine (C-X-C Motif) Receptor 7 (CXCR7) antibodyCXCR7 Reactivity: Human FACS Host: Mouse Monoclonal 10D1-J16 unconjugated
anti-Chemokine (C-X-C Motif) Receptor 7 (CXCR7) (AA 333-362), (C-Term) antibodyCXCR7 Reactivity: Human WB, FACS Host: Rabbit Polyclonal RB24391 unconjugated
anti-Chemokine (C-X-C Motif) Receptor 7 (CXCR7) (Cytoplasmic Domain) antibodyCXCR7 Reactivity: Human, Horse WB, IHC, IHC (p) Host: Rabbit Polyclonal unconjugated
anti-Chemokine (C-X-C Motif) Receptor 7 (CXCR7) (AA 311-360) antibodyCXCR7 Reactivity: Human, Mouse, Rat WB, ELISA, IHC, IHC (p), IF Host: Rabbit Polyclonal unconjugated
-
- Application Notes
- Approved: IHC, IHC-P (10 μg/mL)
- Comment
-
Target Species of Antibody: Human
- Assay Procedure
-
The IHC-pro Immunohistochemistry Protocol
Tissue Preparation
Formalin fixation and embedding in paraffin wax
Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.
Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.
Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.
Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.
Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip - Restrictions
- For Research Use only
-
- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- PBS, less than 0.1 % sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
- Expiry Date
- 12 months
-
- Target
- CXCR7 (Chemokine (C-X-C Motif) Receptor 7 (CXCR7))
- Alternative Name
- ACKR3 / CXCR7 (CXCR7 Products)
- Synonyms
- CMKOR1 antibody, CXC-R7 antibody, CXCR-7 antibody, CXCR7 antibody, GPR159 antibody, RDC-1 antibody, RDC1 antibody, AW541270 antibody, Cmkor1 antibody, Cxcr7 antibody, Rdc1 antibody, ackr3 antibody, cxcr7 antibody, rdc1 antibody, xcxcr7 antibody, atypical chemokine receptor 3 antibody, ORF 74; similar to Kaposi's sarcoma-associated herpesvirus ORF 74 antibody, atypical chemokine receptor 3 L homeolog antibody, ACKR3 antibody, MmrVgp88 antibody, Ackr3 antibody, ackr3.L antibody
- Background
-
Name/Gene ID: ACKR3
Subfamily: Chemokine
Family: GPCR
Synonyms: ACKR3, Chemokine orphan receptor 1, CMKOR1, CXCR7, CXC-R7, GPRN1, RDC1, RDC-1, CXCR-7, G-protein coupled receptor 159, GPR159 - Gene ID
- 57007
- Pathways
- Myometrial Relaxation and Contraction, Negative Regulation of intrinsic apoptotic Signaling
-
