NR1H3 antibody (C-Term)
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- Target See all NR1H3 Antibodies
- NR1H3 (Nuclear Receptor Subfamily 1, Group H, Member 3 (NR1H3))
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Binding Specificity
- C-Term
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Reactivity
- Human, Mouse, Rat, Cow, Xenopus laevis, Dog, Goat, Horse, Rabbit, Pig, Guinea Pig, Monkey, Zebrafish (Danio rerio), Bat, Chicken, Hamster
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This NR1H3 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Brand
- IHC-plus™
- Specificity
- Human NR1H3 / LXR Alpha
- Predicted Reactivity
- Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Goat, Pig, Guinea pig, Chicken, Zebrafish (100%).
- Purification
- Immunoaffinity purified
- Immunogen
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Synthetic peptide from C-Terminus of human NR1H3 (Q13133, NP_005684). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Goat, Hamster, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Guinea pig, Turkey, Zebra finch, Chicken, Platypus, Lizard, Xenopus, Trout, Salmon, Stickleback, Zebrafish (100%).
Type of Immunogen: Synthetic peptide - Isotype
- IgG
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anti-Nuclear Receptor Subfamily 1, Group H, Member 3 (NR1H3) (AA 1-387) antibody
NR1H3 Reactivity: Human WB, ELISA Host: Mouse Monoclonal 3E9 unconjugated
anti-Nuclear Receptor Subfamily 1, Group H, Member 3 (NR1H3) (AA 50-330) antibodyNR1H3 Reactivity: Human WB Host: Rabbit Polyclonal unconjugated
anti-Nuclear Receptor Subfamily 1, Group H, Member 3 (NR1H3) (AA 1-402) antibodyNR1H3 Reactivity: Human WB, ELISA, IHC Host: Rabbit Polyclonal unconjugated
anti-Nuclear Receptor Subfamily 1, Group H, Member 3 (NR1H3) (C-Term) antibodyNR1H3 Reactivity: Human, Mouse, Rat WB, ELISA, IHC, ICC, IF Host: Rabbit Polyclonal unconjugated
anti-Nuclear Receptor Subfamily 1, Group H, Member 3 (NR1H3) (AA 226-253) antibodyNR1H3 Reactivity: Human WB, FACS, IF Host: Rabbit Polyclonal RB23158 unconjugated
anti-Nuclear Receptor Subfamily 1, Group H, Member 3 (NR1H3) (AA 1-402) antibodyNR1H3 Reactivity: Human WB, IF Host: Mouse Polyclonal unconjugated
anti-Nuclear Receptor Subfamily 1, Group H, Member 3 (NR1H3) (AA 50-150) antibodyNR1H3 Reactivity: Human, Mouse, Rat, Monkey, Chicken, Primate WB Host: Rabbit Polyclonal unconjugated
anti-Nuclear Receptor Subfamily 1, Group H, Member 3 (NR1H3) (N-Term) antibodyNR1H3 Reactivity: Human, Mouse WB, ChIP-seq, ChIP Host: Rabbit Polyclonal unconjugated
anti-Nuclear Receptor Subfamily 1, Group H, Member 3 (NR1H3) (AA 95-434) antibodyNR1H3 Reactivity: Human WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated
anti-Nuclear Receptor Subfamily 1, Group H, Member 3 (NR1H3) (AA 93-432) antibodyNR1H3 Reactivity: Mouse WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated
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- Application Notes
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Approved: IHC, IHC-P (5 μg/mL), WB
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. ELISA titer using peptide based assay: 1:62500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. - Comment
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Target Species of Antibody: Human
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Distilled water
- Concentration
- Lot specific
- Buffer
- Lyophilized from PBS with 2 % sucrose
- Handling Advice
- Avoid repeat freeze-thaw cycles.
- Storage
- 4 °C,-20 °C
- Storage Comment
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Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
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- Target
- NR1H3 (Nuclear Receptor Subfamily 1, Group H, Member 3 (NR1H3))
- Alternative Name
- NR1H3 / LXR Alpha (NR1H3 Products)
- Synonyms
- NR1H3 antibody, LXR-a antibody, LXRA antibody, RLD-1 antibody, AU018371 antibody, LXR antibody, RLD1 antibody, Unr1 antibody, LXRalpha antibody, LXR-alpha antibody, im:7141239 antibody, wu:fd59b01 antibody, zgc:113339 antibody, zgc:158542 antibody, nuclear receptor subfamily 1 group H member 3 antibody, nuclear receptor subfamily 1, group H, member 3 antibody, NR1H3 antibody, nr1h3 antibody, Nr1h3 antibody
- Background
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Name/Gene ID: NR1H3
Subfamily: NR1 Thyroid hormone-like
Family: NHR
Synonyms: NR1H3, Liver X receptor alpha, LXRA, LXR-a, Oxysterols receptor LXR-alpha, LXR alpha, Lxralpha, RLD-1 - Gene ID
- 10062
- NCBI Accession
- NP_005684
- UniProt
- Q13133
- Pathways
- Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Nuclear Hormone Receptor Binding, Cellular Response to Molecule of Bacterial Origin, Hepatitis C
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