ARPC2 antibody
(1 validation)-
- Target See all ARPC2 Antibodies
- ARPC2 (Actin Related Protein 2/3 Complex, Subunit 2, 34kDa (ARPC2))
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This ARPC2 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Cross-Reactivity
- Human, Mouse, Rat, Zebrafish (Danio rerio)
- Characteristics
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Rabbit Polyclonal antibody to ARPC2 (actin related protein 2/3 complex, subunit 2, 34 kDa)
ARPC2 antibody - Purification
- Purified by antigen-affinity chromatography.
- Immunogen
- Recombinant protein encompassing a sequence within the center region of human ARPC2. The exact sequence is proprietary.
- Isotype
- IgG
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anti-Actin Related Protein 2/3 Complex, Subunit 2, 34kDa (ARPC2) (AA 1-300) antibody
ARPC2 Reactivity: Human WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated
anti-Actin Related Protein 2/3 Complex, Subunit 2, 34kDa (ARPC2) (C-Term) antibodyVerified ARPC2 Reactivity: Human WB, ELISA, IHC, IF Host: Goat Polyclonal unconjugated
anti-Actin Related Protein 2/3 Complex, Subunit 2, 34kDa (ARPC2) (AA 1-300) antibodyARPC2 Reactivity: Human WB, ELISA Host: Mouse Monoclonal 5C8 unconjugated
anti-Actin Related Protein 2/3 Complex, Subunit 2, 34kDa (ARPC2) antibodyARPC2 Reactivity: Human WB, IP, IF Host: Rabbit Monoclonal unconjugated
anti-Actin Related Protein 2/3 Complex, Subunit 2, 34kDa (ARPC2) (AA 1-250) antibodyARPC2 Reactivity: Human ELISA, IHC Host: Rabbit Polyclonal unconjugated
anti-Actin Related Protein 2/3 Complex, Subunit 2, 34kDa (ARPC2) (AA 201-300) antibodyARPC2 Reactivity: Human WB, ELISA, IF (cc), IF (p), ICC, IHC (p), IHC (fro) Host: Rabbit Polyclonal unconjugated
anti-Actin Related Protein 2/3 Complex, Subunit 2, 34kDa (ARPC2) (AA 263-291), (C-Term) antibodyARPC2 Reactivity: Human WB Host: Rabbit Polyclonal RB39330 unconjugated
anti-Actin Related Protein 2/3 Complex, Subunit 2, 34kDa (ARPC2) (N-Term) antibodyARPC2 Reactivity: Human, Mouse, Rat, Cow, Guinea Pig, Horse, Rabbit, Pig, Hamster, Monkey, Xenopus laevis WB Host: Rabbit Polyclonal unconjugated
anti-Actin Related Protein 2/3 Complex, Subunit 2, 34kDa (ARPC2) (N-Term) antibodyARPC2 Reactivity: Human, Mouse, Rat, Cow, Guinea Pig, Horse, Rabbit WB Host: Rabbit Polyclonal unconjugated
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- Application Notes
- WB: 1:500-1:5000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.
- Comment
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Positive Control: rat kidney , mouse kidney
- Restrictions
- For Research Use only
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- by
- Akoya Biosciences
- No.
- #104342
- Date
- 04/20/2021
- Antigen
- ARPC2
- Lot Number
- 39911
- Method validated
- Multiplex Immunohistochemistry
- Positive Control
FFPE normal human cortex
- Negative Control
Unlabeled control (FFPE normal human cortex)
- Notes
Passed. The anti-ARPC2 antibody ABIN2855249 produced staining in neuronal processes throughout the human cortex.
- Primary Antibody
- ABIN2855249
- Secondary Antibody
- Full Protocol
- Protocol details are described in the Akoya Biosciences CODEX® User Manual (see https://www.akoyabio.com/wp-content/uploads/2021/01/CODEX-User-Manual.pdf).
- Tissue preparation as outlined in the Akoya Biosciences CODEX® User Manual fresh-frozen tissue protocol.
- Conjugation of the anti-ARPC2 antibody ABIN2855249 to an oligo barcode used to bind oligo-conjugated fluorophore AF488.
- Experimental Notes
No signal was detected in unlabeled specimens.
Specific staining of ARPC2 was observed with human FFPE cortical tissue sections with tris-EDTA antigen retrieval.
Anti-ARPC antibody ABIN2855249 was weakly compatible with staining of fresh frozen mouse tissue
- Optimal staining and signal to noise ratios were obtained if tissue was pre-treated for autofluorescence removal (see https://www.akoyabio.com/wp-content/uploads/2020/07/Customer-Demonstrated-Protocol-Autofluorescence-Quenching-Mar2020.pdf).
Validation #104342 (Multiplex Immunohistochemistry)!['Independent Validation' Badge]( "Successfully validated")
!['Independent Validation' Badge]( "Successfully validated")
Validation Images![FFPE normal human cortex tissue section labeled with anti-ARPC2 antibody ABIN2855249 (cyan; conjugated to fluorophore AF488) after EDTA antigen retrieval. MAP2, neurofilament, and GFAP were labeled with anti-MAP2 antibody ABIN125739 (green; conjugated to fluorophore ATTO 550), anti-neurofilament antibody (red; conjugated to fluorophore AF488), and anti-GFAP antibody (purple; conjugated to fluorophore AF647) respectively.]()
FFPE normal human cortex tissue section labeled with anti-ARPC2 antibody ABIN2855249 (cyan; conjugated to fluorophore AF488) after EDTA antigen retrieval. MAP2, neurofilament, and GFAP were labeled with anti-MAP2 antibody ABIN125739 (green; conjugated to fluorophore ATTO 550), anti-neurofilament antibody (red; conjugated to fluorophore AF488), and anti-GFAP antibody (purple; conjugated to fluorophore AF647) respectively.
![FFPE normal human cortex tissue section labeled with anti-ARPC2 antibody ABIN2855249 (cyan; conjugated to fluorophore AF488) after EDTA antigen retrieval. GFAP was labeled with anti-ARPC2 antibody (red; conjugated to fluorophore AF647).]()
FFPE normal human cortex tissue section labeled with anti-ARPC2 antibody ABIN2855249 (cyan; conjugated to fluorophore AF488) after EDTA antigen retrieval. GFAP was labeled with anti-ARPC2 antibody (red; conjugated to fluorophore AF647).
Full Methods -
- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- 1XPBS ( pH 7), 20 % Glycerol, 0.025 % ProClin 300
- Preservative
- ProClin
- Precaution of Use
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
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- Target
- ARPC2 (Actin Related Protein 2/3 Complex, Subunit 2, 34kDa (ARPC2))
- Alternative Name
- actin related protein 2/3 complex subunit 2 (ARPC2 Products)
- Synonyms
- arc34 antibody, p34-arc antibody, pnas-139 antibody, pro2446 antibody, Arpc2 antibody, Arc-p34 antibody, 2210023N03Rik antibody, 34kDa antibody, p34-Arc antibody, fk84a07 antibody, wu:fa22f07 antibody, wu:fk84a07 antibody, zgc:77769 antibody, ARC34 antibody, PNAS-139 antibody, actin related protein 2/3 complex subunit 2 antibody, actin related protein 2/3 complex, subunit 2, 34kDa antibody, actin related protein 2/3 complex, subunit 2 antibody, actin related protein 2/3 complex subunit 2 S homeolog antibody, ARPC2 antibody, arpc2 antibody, Arpc2 antibody, arpc2.S antibody
- Background
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This gene encodes one of seven subunits of the human Arp2/3 protein complex. The Arp2/3 protein complex has been implicated in the control of actin polymerization in cells and has been conserved through evolution. The exact role of the protein encoded by this gene, the p34 subunit, has yet to be determined. Two alternatively spliced variants have been characterized to date. Additional alternatively spliced variants have been described but their full length nature has not been determined.
Cellular Localization: Cytoplasm , cytoskeleton , Cell projection - Molecular Weight
- 34 kDa
- Gene ID
- 10109
- UniProt
- O15144
- Pathways
- RTK Signaling, Regulation of Actin Filament Polymerization
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