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KAP1 antibody (C-Term)

Name: KAP1 antibody (C-Term)
Brand: antibodies-online
SKU: ABIN184913
Price: 469.28 USD
Availability: InStock

Verified TRIM28 Reactivity: Human ELISA, IHC, IF, FACS Host: Goat Polyclonal unconjugated

ABIN184913 (2µg/ml) staining of paraffin embedded Human Breast.

IHC

ABIN184913 (2 µg/mL) staining of HepG2 lysate (35 µg protein in RIPA buffer).

ABIN184913 (4 µg/mL) staining of paraffin embedded Human Breast.

ABIN184913 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.

ABIN184913 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.

ABIN184913 Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.

FACS

6 images

(1 reference)

Catalog No. ABIN184913

Datasheet as PDF

Target See all KAP1 (TRIM28) Antibodies

KAP1 (TRIM28) (Tripartite Motif Containing 28 (TRIM28))
Binding Specificity
29

15

11

9

8

6

6

5

5

4

2

2

2

2

2

1

1

1

1

1

1

1
C-Term
Reactivity
112

53

49

10

9

8

7

5

4

2

1

1

1
Human
Host
114

15

3
Goat
Compatible Secondaries
Clonality
107

25
Polyclonal
Conjugate
61

10

8

5

5

5

5

5

5

5

4

3

2

2

2

2

2

1
This KAP1 antibody is un-conjugated
Application
85

40

40

27

26

22

21

21

18

15

6

4

4

3

2

2

2

1

1

1
ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Purpose

KAP1 / TRIM28

Sequence

SSQELSGGPG DGP

Cross-Reactivity

Human, Mouse

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade

Verified

Immunogen

Peptide with sequence C-SSQELSGGPGDGP, from the C Terminus of the protein sequence according to NP_005753.1.

Isotype

IgG
anti-Tripartite Motif Containing 28 (TRIM28) antibody
TRIM28 Reactivity: Human WB, IF, IP, IHC (p), ICC, ChIP Host: Rabbit Polyclonal unconjugated

anti-Tripartite Motif Containing 28 (TRIM28) (N-Term) antibody
TRIM28 Reactivity: Human WB, IF, IHC (p), ICC Host: Rabbit Polyclonal unconjugated

anti-Tripartite Motif Containing 28 (TRIM28) (AA 379-524) antibody
TRIM28 Reactivity: Human WB, ELISA, IF, IHC (p), RNAi Host: Mouse Monoclonal 4E6 unconjugated

anti-Tripartite Motif Containing 28 (TRIM28) (AA 379-524) antibody
TRIM28 Reactivity: Human WB, ELISA, IHC (p) Host: Mouse Monoclonal 1D11 unconjugated

anti-Tripartite Motif Containing 28 (TRIM28) (AA 22-291) antibody
TRIM28 Reactivity: Human WB, ELISA, IHC, IP Host: Rabbit Polyclonal unconjugated

anti-Tripartite Motif Containing 28 (TRIM28) antibody
TRIM28 Reactivity: Human WB, IHC, IP, ICC Host: Mouse Monoclonal unconjugated

anti-Tripartite Motif Containing 28 (TRIM28) (AA 178-207), (N-Term) antibody
TRIM28 Reactivity: Human WB, IF, IHC (p) Host: Rabbit Polyclonal RB09760 unconjugated

anti-Tripartite Motif Containing 28 (TRIM28) antibody
TRIM28 Reactivity: Human, Mouse, Rat IHC, IF Host: Rabbit Polyclonal unconjugated

anti-Tripartite Motif Containing 28 (TRIM28) (pSer824) antibody
TRIM28 Reactivity: Human, Mouse, Rat WB, ELISA, IHC Host: Rabbit Polyclonal unconjugated

anti-Tripartite Motif Containing 28 (TRIM28) (AA 1-835) antibody
TRIM28 Reactivity: Human WB, IF Host: Mouse Polyclonal unconjugated

Application Notes

Immunohistochemistry: In paraffin embedded Human Breast shows nuclear staining in epithelial duct cells. Recommended concentration: 2-4 μg/mL.
Peptide ELISA: antibody detection limit dilution 1:2000.

Comment

Immunofluorescence: Strong expression of the protein seen in the nuclei of HeLa and U2OS cells. Recommended concentration: 10μg/ml.
Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10u

Restrictions

For Research Use only
Format

Liquid

Concentration

0.5 mg/mL

Buffer

Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice

Minimize freezing and thawing.

Storage

-20 °C

Storage Comment

Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
Yokoe, Toiyama, Okugawa, Tanaka, Ohi, Inoue, Mohri, Miki, Kusunoki: "KAP1 is associated with peritoneal carcinomatosis in gastric cancer." in: Annals of surgical oncology, Vol. 17, Issue 3, pp. 821-8, (2010) (PubMed).
Target

KAP1 (TRIM28) (Tripartite Motif Containing 28 (TRIM28))

Alternative Name

TRIM28 (TRIM28 Products)

Synonyms

KAP1 antibody, RNF96 antibody, TF1B antibody, TIF1B antibody, TRIM28 antibody, kap1 antibody, rnf96 antibody, tf1b antibody, tif1b antibody, AA408787 antibody, KAP-1 antibody, KRIP-1 antibody, Tif1b antibody, Tif1beta antibody, Kap1 antibody, Krip1 antibody, TIF1BETA antibody, tripartite motif containing 28 antibody, tripartite motif containing 28 L homeolog antibody, tripartite motif-containing 28 antibody, TRIM28 antibody, trim28.L antibody, Trim28 antibody

Background

TRIM28, KAP1, TF1B, RNF96, TIF1B, tripartite motif-containing 28, KRAB-associated protein 1, nuclear corepressor KAP-1, transcriptional intermediary factor 1-beta, FLJ29029, KRAB-associated protein 1, tripartite motif-containing 28 protein

Gene ID

10155

NCBI Accession

NP_005753

Pathways

Hedgehog Signaling, Positive Regulation of Response to DNA Damage Stimulus