MAPKAP Kinase 3 antibody (C-Term)
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- Target See all MAPKAP Kinase 3 (MAPKAPK3) Antibodies
- MAPKAP Kinase 3 (MAPKAPK3) (Mitogen-Activated Protein Kinase-Activated Protein Kinase 3 (MAPKAPK3))
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Binding Specificity
- C-Term
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Reactivity
- Human, Mouse, Rat
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This MAPKAP Kinase 3 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)
- Specificity
- MAPK3 Antibody detects endogenous levels of total MAPK3.
- Predicted Reactivity
- Pig,Bovine,Horse,Sheep,Rabbit,Dog,Chicken
- Purification
- The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
- Immunogen
- A synthesized peptide derived from human MAPK3, corresponding to a region within C-terminal amino acids.
- Isotype
- IgG
- Top Product
- Discover our top product MAPKAPK3 Primary Antibody
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anti-Mitogen-Activated Protein Kinase-Activated Protein Kinase 3 (MAPKAPK3) (AA 272-382) antibody
MAPKAPK3 Reactivity: Human WB, ELISA, IHC (p), PLA Host: Mouse Monoclonal 2B5 unconjugated
anti-Mitogen-Activated Protein Kinase-Activated Protein Kinase 3 (MAPKAPK3) (AA 272-382) antibodyMAPKAPK3 Reactivity: Human WB, ELISA, IP Host: Mouse Monoclonal 3F4 unconjugated
anti-Mitogen-Activated Protein Kinase-Activated Protein Kinase 3 (MAPKAPK3) (AA 1-240) antibodyMAPKAPK3 Reactivity: Human WB, IHC, ELISA Host: Rabbit Polyclonal unconjugated
anti-Mitogen-Activated Protein Kinase-Activated Protein Kinase 3 (MAPKAPK3) antibodyKO Validated MAPKAPK3 Reactivity: Human WB, IF, ICC Host: Rabbit Polyclonal unconjugated
anti-Mitogen-Activated Protein Kinase-Activated Protein Kinase 3 (MAPKAPK3) antibodyMAPKAPK3 Reactivity: Human, Mouse, Rat IHC, ELISA Host: Rabbit Polyclonal unconjugated
anti-Mitogen-Activated Protein Kinase-Activated Protein Kinase 3 (MAPKAPK3) (AA 1-382) antibodyMAPKAPK3 Reactivity: Human WB, IF Host: Rabbit Polyclonal unconjugated
anti-Mitogen-Activated Protein Kinase-Activated Protein Kinase 3 (MAPKAPK3) (AA 129-329) antibodyMAPKAPK3 Reactivity: Human WB, IHC, ICC Host: Rabbit Polyclonal unconjugated
anti-Mitogen-Activated Protein Kinase-Activated Protein Kinase 3 (MAPKAPK3) (C-Term) antibodyMAPKAPK3 Reactivity: Human, Pig WB, IHC, IF, IC Host: Rabbit Polyclonal unconjugated
anti-Mitogen-Activated Protein Kinase-Activated Protein Kinase 3 (MAPKAPK3) (AA 63-90), (N-Term) antibodyMAPKAPK3 Reactivity: Mouse WB, IHC (p) Host: Rabbit Polyclonal unconjugated
anti-Mitogen-Activated Protein Kinase-Activated Protein Kinase 3 (MAPKAPK3) (C-Term) antibodyMAPKAPK3 Reactivity: Human WB, IHC, ELISA, IF Host: Rabbit Polyclonal unconjugated
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- Application Notes
- WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C
- Storage Comment
- Store at -20 °C. Stable for 12 months from date of receipt.
- Expiry Date
- 12 months
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- Target
- MAPKAP Kinase 3 (MAPKAPK3) (Mitogen-Activated Protein Kinase-Activated Protein Kinase 3 (MAPKAPK3))
- Alternative Name
- MAPKAPK3 (MAPKAPK3 Products)
- Synonyms
- 3PK antibody, MAPKAP-K3 antibody, MAPKAP3 antibody, MAPKAPK-3 antibody, MK-3 antibody, AI874665 antibody, MK3 antibody, MapkKapk3 antibody, MAPKAPK3 antibody, mapkapk3 antibody, MGC154360 antibody, 3pk antibody, mapkap3 antibody, zgc:158342 antibody, mitogen-activated protein kinase-activated protein kinase 3 antibody, mitogen-activated protein kinase-activated protein kinase 3 S homeolog antibody, MAPKAPK3 antibody, Mapkapk3 antibody, mapkapk3.S antibody, mapkapk3 antibody
- Background
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Description: Stress-activated serine/threonine-protein kinase involved in cytokines production, endocytosis, cell migration, chromatin remodeling and transcriptional regulation. Following stress, it is phosphorylated and activated by MAP kinase p38-alpha/MAPK14, leading to phosphorylation of substrates. Phosphorylates serine in the peptide sequence, Hyd-X-R-X2-S, where Hyd is a large hydrophobic residue. MAPKAPK2 and MAPKAPK3, share the same function and substrate specificity, but MAPKAPK3 kinase activity and level in protein expression are lower compared to MAPKAPK2. Phosphorylates HSP27/HSPB1, KRT18, KRT20, RCSD1, RPS6KA3, TAB3 and TTP/ZFP36. Mediates phosphorylation of HSP27/HSPB1 in response to stress, leading to dissociate HSP27/HSPB1 from large small heat-shock protein (sHsps) oligomers and impair their chaperone activities and ability to protect against oxidative stress effectively. Involved in inflammatory response by regulating tumor necrosis factor (TNF) and IL6 production post-transcriptionally: acts by phosphorylating AU-rich elements (AREs)-binding proteins, such as TTP/ZFP36, leading to regulate the stability and translation of TNF and IL6 mRNAs. Phosphorylation of TTP/ZFP36, a major post-transcriptional regulator of TNF, promotes its binding to 14-3-3 proteins and reduces its ARE mRNA affinity leading to inhibition of dependent degradation of ARE-containing transcript. Involved in toll-like receptor signaling pathway (TLR) in dendritic cells: required for acute TLR-induced macropinocytosis by phosphorylating and activating RPS6KA3. Also acts as a modulator of Polycomb-mediated repression.
Gene: MAPKAPK3
- Molecular Weight
- 42kDa
- Gene ID
- 7867
- UniProt
- Q16644
- Pathways
- MAPK Signaling, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades
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