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CDKN1B antibody (AA 150-198)

Name: CDKN1B antibody (AA 150-198)
SKU: ABIN672621
Price: 384.62 USD
Availability: InStock

CDKN1B Reactivity: Human, Mouse, Rat WB, ELISA, FACS, IHC (p), IF (cc), IF (p), IHC (fro) Host: Rabbit Polyclonal unconjugated

Dnmt3a3 overexpression decreases fat accumulation in differentiating preadipocytes.

Formalin-fixed and paraffin embedded human ovary carcinoma labeled Anti-CDKN1B/P27kip1 Polyclonal Antibody, Unconjugated (ABIN672621) at 1:200, followed by conjugation to the secondary antibody and DAB staining

IHC

Mouse lung lysates probed with CDKN1B Polyclonal Antibody, unconjugated at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 60 minutes at 37°C.

MCF-7 cells were stained with CDKN1B Polyclonal Antibody, Unconjugated at 1:500 in PBS and incubated for two hours at 37°C followed by Goat Anti-Rabbit IgG (H+L) Cy3 conjugated secondary antibody.

IF (cc)

Formalin-fixed and paraffin embedded human ovary carcinoma labeled Anti-CDKN1B/P27kip1 Polyclonal Antibody, Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining

IHC (p)

RSC96 probed with CDKN1B Polyclonal Antibody, Unconjugated at 1:100 for 30 minutes followed by incubation with a conjugated secondary (PE Conjugated) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).

FACS

Expression of Dnmt3a spliced variants in adipose tissues and during the time-course analysis of preadipocyte differentiation.

7 images

(5 references)

Catalog No. ABIN672621

Datasheet as PDF

Target See all CDKN1B Antibodies

CDKN1B (Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B))
Binding Specificity
29

28

22

19

17

15

11

10

10

8

8

8

8

8

7

7

5

3

3

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 150-198
Reactivity
273

125

105

26

6

6

3

3

3

3

2

1

1
Human, Mouse, Rat
Host
239

49

2

1

1
Rabbit
Compatible Secondaries
Clonality
231

61
Polyclonal
Conjugate
142

24

17

16

16

14

6

6

6

6

6

6

3

2

2

2

2

2

2

2

2

2

2

2

2
This CDKN1B antibody is un-conjugated
Application
201

133

71

61

51

41

35

26

22

15

15

13

7

6

3

1

1

1
Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity

Chicken, Human, Mouse, Rat

Predicted Reactivity

Dog,Sheep,Pig

Purification

Purified by Protein A.

Immunogen

KLH conjugated synthetic peptide derived from human CDKN1B

Isotype

IgG
anti-Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B) (AA 1-198) antibody
CDKN1B Reactivity: Human WB, ELISA, IF, IHC (p), PLA Host: Mouse Monoclonal 4B4-E6 unconjugated

anti-Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B) antibody
KO Validated CDKN1B Reactivity: Human WB, IHC, IF, IHC (p), IP, ICC, IHC (fro) Host: Rabbit Polyclonal unconjugated

anti-Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B) antibody
CDKN1B Reactivity: Human, Mouse, Rat WB, IHC Host: Rabbit Polyclonal unconjugated

anti-Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B) antibody
CDKN1B Reactivity: Human IF, FACS, IHC (f) Host: Mouse Monoclonal SX53G8 unconjugated

anti-Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B) antibody
CDKN1B Reactivity: Human, Mouse, Rat IHC, IF Host: Rabbit Polyclonal unconjugated

anti-Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B) antibody
CDKN1B Reactivity: Human WB, ELISA, IHC, IF Host: Rabbit Monoclonal 1G2 unconjugated Recombinant Antibody

anti-Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B) (pThr187) antibody
CDKN1B Reactivity: Human, Mouse, Rat WB, ELISA, IHC, IF, ICC Host: Rabbit Polyclonal unconjugated

anti-Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B) (AA 1-198) antibody
CDKN1B Reactivity: Human WB, IF Host: Mouse Polyclonal unconjugated

anti-Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B) antibody
CDKN1B Reactivity: Mouse WB, IF, FACS, IHC (f) Host: Mouse Monoclonal DCS-72-F6 unconjugated

anti-Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B) (AA 1-100) antibody
CDKN1B Reactivity: Human WB, IHC, IF Host: Rabbit Polyclonal unconjugated

Application Notes

WB 1:100-1000
FCM 1:20-100
IHC-P 1:100-500
IF(IHC-P) 1:50-200

Restrictions

For Research Use only
Format

Liquid

Concentration

1 μg/μL

Buffer

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage

4 °C,-20 °C

Storage Comment

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date

12 months
Abdalla, Li, Nie: "A Novel DNA Methyltransferase Dnmt3a3 Splice Variant Represses Preadipocyte Proliferation and Differentiation." in: Frontiers in genetics, Vol. 11, pp. 115, (2020) (PubMed).

Guo, Xu, Ren, Zhang, Zhang, Duan, Zhou, Sun: "Endosulfan induces apoptosis by activating the negative regulation pathway of cell cycle and death receptor pathway in spermatogenic cells." in: Toxicology research, Vol. 6, Issue 2, pp. 223-231, (2017) (PubMed).

Akizuki, Shimobaba, Matsunaga, Endo, Ikari: "Claudin-5, -7, and -18 suppress proliferation mediated by inhibition of phosphorylation of Akt in human lung squamous cell carcinoma." in: Biochimica et biophysica acta, Vol. 1864, Issue 2, pp. 293-302, (2016) (PubMed).

Gao, Tang, Ding, Wang, Qi, Wu, Guo: "Protein-Binding Function of RNA-Dependent Protein Kinase Promotes Proliferation through TRAF2/RIP1/NF-?B/c-Myc Pathway in Pancreatic ? cells." in: Molecular medicine (Cambridge, Mass.), Vol. 21, Issue 1, pp. 154-66, (2015) (PubMed).

Sun, Yang, Luo, Wang, Chen, Zhang, Wang, Li: "Thyroid hormone inhibits the proliferation of piglet Sertoli cell via PI3K signaling pathway." in: Theriogenology, (2014) (PubMed).
Target

CDKN1B (Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B))

Alternative Name

CDKN1B (CDKN1B Products)

Synonyms

CDKN4 antibody, KIP1 antibody, MEN1B antibody, MEN4 antibody, P27KIP1 antibody, cdkn1b antibody, sb:cb611 antibody, wu:fb63g10 antibody, wu:fb64g10 antibody, wu:fe18e03 antibody, CDKN1B antibody, Kip1 antibody, p27 antibody, FAM14D antibody, ISG12 antibody, ISG12A antibody, P27 antibody, Cdki1b antibody, AA408329 antibody, AI843786 antibody, p27Kip1 antibody, cdkn1bl antibody, cyclin dependent kinase inhibitor 1B antibody, cyclin-dependent kinase inhibitor 1Bb antibody, interferon alpha inducible protein 27 antibody, cyclin-dependent kinase inhibitor 1B antibody, cyclin-dependent kinase inhibitor 1Ba antibody, CDKN1B antibody, cdkn1bb antibody, IFI27 antibody, Cdkn1b antibody, cdkn1ba antibody

Background

Synonyms: KIP1, MEN4, CDKN4, MEN1B, P27KIP1, Cyclin-dependent kinase inhibitor 1B, Cyclin-dependent kinase inhibitor p27, CDKN1B
Background: Important regulator of cell cycle progression. Involved in G1 arrest. Potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. Forms a complex with cyclin type D-CDK4 complexes and is involved in the assembly, stability, and modulation of CCND1-CDK4 complex activation. Acts either as an inhibitor or an activator of cyclin type D-CDK4 complexes depending on its phosphorylation state and/or stoichometry.

Gene ID

1027

UniProt

P46527

Pathways

Cell Division Cycle, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Negative Regulation of Hormone Secretion, Sensory Perception of Sound, Mitotic G1-G1/S Phases, DNA Replication, Positive Regulation of Endopeptidase Activity, Synthesis of DNA, Autophagy