CITED2 antibody (N-Term)
(1 validation)-
- Target See all CITED2 Antibodies
- CITED2 (Cbp/p300-Interacting Transactivator, with Glu/Asp-Rich Carboxy-terminal Domain, 2 (CITED2))
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Binding Specificity
- N-Term
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Reactivity
- Human, Mouse, Rat, Cow, Dog, Guinea Pig, Sheep
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This CITED2 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (IHC)
- Sequence
- HIHYGAGNMN ATSGIRHAMG PGTVNGGHPP SALAPAARFN NSQFMGPPVA
- Predicted Reactivity
- Cow: 100%, Dog: 100%, Guinea Pig: 100%, Human: 100%, Mouse: 100%, Rat: 100%, Sheep: 100%
- Characteristics
- This is a rabbit polyclonal antibody against CITED2. It was validated on Western Blot using a cell lysate as a positive control.
- Purification
- Affinity Purified
- Immunogen
- The immunogen is a synthetic peptide directed towards the N terminal region of human CITED2
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anti-Cbp/p300-Interacting Transactivator, with Glu/Asp-Rich Carboxy-terminal Domain, 2 (CITED2) antibody
CITED2 Reactivity: Human, Mouse, Rat WB Host: Rabbit Polyclonal unconjugated
anti-Cbp/p300-Interacting Transactivator, with Glu/Asp-Rich Carboxy-terminal Domain, 2 (CITED2) (AA 200-226), (C-Term) antibodyCITED2 Reactivity: Human, Mouse WB, IF Host: Rabbit Polyclonal RB23441 unconjugated
anti-Cbp/p300-Interacting Transactivator, with Glu/Asp-Rich Carboxy-terminal Domain, 2 (CITED2) (N-Term) antibodyCITED2 Reactivity: Human WB, IHC, IHC (p) Host: Rabbit Polyclonal unconjugated
anti-Cbp/p300-Interacting Transactivator, with Glu/Asp-Rich Carboxy-terminal Domain, 2 (CITED2) (N-Term) antibodyCITED2 Reactivity: Human, Mouse, Rat, Cow, Pig, Dog, Guinea Pig, Sheep WB Host: Rabbit Polyclonal unconjugated
anti-Cbp/p300-Interacting Transactivator, with Glu/Asp-Rich Carboxy-terminal Domain, 2 (CITED2) (Internal Region) antibodyCITED2 Reactivity: Human, Mouse, Rat WB, ELISA, IF, ICC Host: Rabbit Polyclonal unconjugated
anti-Cbp/p300-Interacting Transactivator, with Glu/Asp-Rich Carboxy-terminal Domain, 2 (CITED2) antibodyCITED2 Reactivity: Human, Mouse ELISA, IHC Host: Rabbit Polyclonal unconjugated
anti-Cbp/p300-Interacting Transactivator, with Glu/Asp-Rich Carboxy-terminal Domain, 2 (CITED2) (AA 1-270) antibodyCITED2 Reactivity: Human WB Host: Mouse Polyclonal unconjugated
anti-Cbp/p300-Interacting Transactivator, with Glu/Asp-Rich Carboxy-terminal Domain, 2 (CITED2) (N-Term) antibodyCITED2 Reactivity: Human, Mouse, Rat, Cow, Pig, Monkey WB Host: Rabbit Polyclonal unconjugated
anti-Cbp/p300-Interacting Transactivator, with Glu/Asp-Rich Carboxy-terminal Domain, 2 (CITED2) (AA 55-104) antibodyCITED2 Reactivity: Human, Mouse, Rat, Cow, Pig, Guinea Pig, Monkey, Bat WB Host: Rabbit Polyclonal unconjugated
anti-Cbp/p300-Interacting Transactivator, with Glu/Asp-Rich Carboxy-terminal Domain, 2 (CITED2) (AA 1-275) antibodyCITED2 Reactivity: Human WB Host: Rabbit Polyclonal unconjugated
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- Application Notes
- Optimal working dilutions should be determined experimentally by the investigator.
- Comment
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Antigen size: 270 AA
- Restrictions
- For Research Use only
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- by
- Dr. Pengpeng Bi Lab, Department of Genetics, Franklin College of Arts and Sciences, University of Georgia
- No.
- #104256
- Date
- 02/10/2020
- Antigen
- CITED2
- Lot Number
- QC11500
- Method validated
- Western Blotting
- Positive Control
HEK293 cells overexpressing murine Cited2 (NCBI protein ID: NP_034958, 269 amino acids) from a transiently transfected expression plasmid
- Negative Control
Transient transfection with empty vector
- Notes
Passed. ABIN2776451 specifically recognizes Cited2 in human HEK293 cell lysates.
- Primary Antibody
- ABIN2776451
- Secondary Antibody
- Goat anti-Rabbit IgG (H+L), Superclonal Recombinant Secondary Antibody, HRP (Invitrogen, A27036, lot 20310171)
- Full Protocol
- Grow human HEK293 cells (Cell Biolabs, RV-101) in DMEM medium(Sigma, D5796) supplemented with FBS (Gemini, A59G00J) and Pen Strep (Gibco, 15140-122, lot 1999384), at 37°C and 5% CO2 in 2mL medium on a 6 well plate to 80% confluency.
- Transfect cells with 2µg per well pMXs-Puro murine Cited2 expression plasmid using PEI (Polysciences, 23966-1) following the manufacturer´s instructions.
- Lyse 1x106 cells in 100µl per well cold RIPA (Sigma, R0278).
- Determine total protein content of the lysates using Pierce BCA Protein Assay Kit (Thermo Fisher, 23225).
- Mix 40 µg of total protein with in 10 µL 4x Laemmli Sample Buffer (Biorad, 1610747) and subsequently separate them on a denaturing 12% PAGE gel (Biorad, 1610156) in a Biorad electrophoresis chamber for 1h at 100V.
- Transfer proteins onto Immobilon-PSQ PVDF Membrane (Sigma, ISEQ00010, lot R8CA8256B) with a Western blotting system for 1h at 110V.
- Block the membrane with blocking buffer (5% non-fat milk in TBST) for 1h at RT.
- Incubation with primary rabbit anti-CITED2 antibody (antibodies-online, ABIN2776451, lot QC11500) diluted 1:200 in blocking buffer ON at 4°C.
- Wash membrane 3x for 15min with TBST.
- Incubation with Goat anti-Rabbit IgG (H+L), Superclonal Recombinant Secondary Antibody, HRP (Invitrogen, A27036, lot 20310171) diluted 1:2000 in blocking buffer for 1h at RT.
- Wash membrane 5x for 15min with TBST.
- Reveal protein bands using SuperSignal West Dura (Thermo Scientific, 34075, TG268239) on a UVP ChemStudio touch (Analytik Jena, 84997092802).
- Experimental Notes
The CITED2 antibody ABIN2776451 reveals a protein of the expected molecular weight of the antigen in lysates of human HEK293 cells expressing murine Cited2. The protein band is only visible in the positive but not the negative controls.
Validation #104256 (Western Blotting)!['Independent Validation' Badge]( "Successfully validated")
!['Independent Validation' Badge]( "Successfully validated")
Validation Images![Western blot analysis using ABIN2776451 of cell lysates from HEK293 cells transfected with an empty vector (EV) or a murine Cited2 expression plasmid (mCited2).]()
Western blot analysis using ABIN2776451 of cell lysates from HEK293 cells transfected with an empty vector (EV) or a murine Cited2 expression plasmid (mCited2).
Full Methods -
- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -20 °C
- Storage Comment
- For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
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- Target
- CITED2 (Cbp/p300-Interacting Transactivator, with Glu/Asp-Rich Carboxy-terminal Domain, 2 (CITED2))
- Alternative Name
- CITED2 (CITED2 Products)
- Synonyms
- mrg1 antibody, p35srj antibody, zgc:103418 antibody, CITED2 antibody, cite2 antibody, ASD8 antibody, MRG-1 antibody, MRG1 antibody, P35SRJ antibody, VSD2 antibody, AI835299 antibody, ER154-like antibody, Mrg1 antibody, Msg2 antibody, Cbp/p300-interacting transactivator, with Glu/Asp rich carboxy-terminal domain, 2 antibody, Cbp/p300-interacting transactivator, with Glu/Asp-rich carboxy-terminal domain, 2 antibody, Cbp/p300 interacting transactivator with Glu/Asp rich carboxy-terminal domain 2 antibody, Cbp/p300-interacting transactivator 2 antibody, Cbp/p300-interacting transactivator, with Glu/Asp rich carboxy-terminal domain, 2 L homeolog antibody, cited2 antibody, CITED2 antibody, cite2 antibody, Cited2 antibody, cited2.L antibody
- Background
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CITED2 belongs to the CITED family. It interferes with the binding of transcription factors HIF-1a and STAT2 to p300/CBP.
Alias Symbols: MRG1, P35SRJ, ASD8, VSD2
Protein Interaction Partner: UBC, ELAVL1, EP300, SMAD3, SMAD2, LHX2, HIF1A, TFAP2C, LHX3, CREBBP, TFAP2A, TBP, TFAP2B,
Protein Size: 270 - Molecular Weight
- 28 kDa
- Gene ID
- 10370
- NCBI Accession
- NM_006079, NP_006070
- UniProt
- Q99967
- Pathways
- Tube Formation
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