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ANAPC10 antibody (AA 1-185)

Name: ANAPC10 antibody (AA 1-185)
Brand: antibodies-online
SKU: ABIN5542270
Price: 511.5 USD
Availability: InStock

ANAPC10 Reactivity: Human WB, IHC, ELISA, ICC, FACS Host: Mouse Monoclonal 8F1D10 unconjugated

Western blot analysis using ANAPC10 mAb against human ANAPC10 (AA: 1-185) recombinant protein.

Immunofluorescence analysis of Hela cells using ANAPC10 mouse mAb.

Immunofluorescence analysis of Hela cells using ANAPC10 mouse mAb (green).

Immunohistochemical analysis of paraffin-embedded colon cancer tissues using ANAPC10 mouse mAb with DAB staining.

IHC

Black line: Control Antigen (100 ng),Purple line: Antigen (10 ng), Blue line: Antigen (50 ng), Red line:Antigen (100 ng)

ELISA

Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using ANAPC10 mouse mAb with DAB staining.

IHC

Western blot analysis using ANAPC10 mAb against HEK293 (1) and ANAPC10 (AA: 1-185)-hIgGFc transfected HEK293 (2) cell lysate.

Western blot analysis using ANAPC10 mouse mAb against Hela (1), MCF-7 (2), SK-Br-3 (3), A431 (4), HEK293 (5), A549 (6), and SPC-A-1 (7) cell lysate.

Flow cytometric analysis of Hela cells using ANAPC10 mouse mAb (green) and negative control (red).

FACS

9 images

Catalog No. ABIN5542270

Datasheet as PDF

Target See all ANAPC10 Antibodies

ANAPC10 (Anaphase Promoting Complex Subunit 10 (ANAPC10))
Binding Specificity
15

6

3

1

1

1

1
AA 1-185
Reactivity
22

19

5

2

2

2

2

2

1

1

1

1

1

1
Human
Host
34

3
Mouse
Compatible Secondaries
Clonality
35

1
Monoclonal
Conjugate
16

2

2

2

2

2

2

2

1

1

1

1

1

1

1
This ANAPC10 antibody is un-conjugated
Application
28

13

13

8

5

4

3

3

3

1

1

1

1
Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunocytochemistry (ICC), Flow Cytometry (FACS)

Purification

purified

Immunogen

Purified recombinant fragment of human ANAPC10 (AA: 1-185) expressed in E. coli.

Clone

8F1D10

Isotype

IgG1
anti-Anaphase Promoting Complex Subunit 10 (ANAPC10) (AA 1-185) antibody
ANAPC10 Reactivity: Human WB, IP Host: Rabbit Polyclonal unconjugated

anti-Anaphase Promoting Complex Subunit 10 (ANAPC10) (AA 1-185) antibody
ANAPC10 Reactivity: Human WB, IF Host: Mouse Polyclonal unconjugated

anti-Anaphase Promoting Complex Subunit 10 (ANAPC10) (N-Term) antibody
ANAPC10 Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Bat, Chicken, Hamster, Monkey, Pig WB Host: Rabbit Polyclonal unconjugated

anti-Anaphase Promoting Complex Subunit 10 (ANAPC10) (N-Term) antibody
ANAPC10 Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish (Danio rerio) WB Host: Rabbit Polyclonal unconjugated

anti-Anaphase Promoting Complex Subunit 10 (ANAPC10) (AA 31-130) antibody
ANAPC10 Reactivity: Rat WB, ELISA, IF (cc), IF (p), IHC (p), ICC, IHC (fro) Host: Rabbit Polyclonal unconjugated

anti-Anaphase Promoting Complex Subunit 10 (ANAPC10) (AA 31-130) antibody (Biotin)
ANAPC10 Reactivity: Rat WB, ELISA, IHC (p), IHC (fro) Host: Rabbit Polyclonal Biotin

anti-Anaphase Promoting Complex Subunit 10 (ANAPC10) (AA 31-130) antibody (Alexa Fluor 680)
ANAPC10 Reactivity: Rat WB, IF (cc), IF (p) Host: Rabbit Polyclonal Alexa Fluor 680

anti-Anaphase Promoting Complex Subunit 10 (ANAPC10) (AA 31-130) antibody (Alexa Fluor 750)
ANAPC10 Reactivity: Rat WB, IF (cc), IF (p) Host: Rabbit Polyclonal Alexa Fluor 750

anti-Anaphase Promoting Complex Subunit 10 (ANAPC10) (AA 31-130) antibody (Alexa Fluor 350)
ANAPC10 Reactivity: Rat WB, IF (cc), IF (p) Host: Rabbit Polyclonal Alexa Fluor 350

anti-Anaphase Promoting Complex Subunit 10 (ANAPC10) (AA 31-130) antibody (Alexa Fluor 647)
ANAPC10 Reactivity: Rat WB, IF (cc), IF (p) Host: Rabbit Polyclonal Alexa Fluor 647

Application Notes

ELISA: 1:10000, WB: 1:500 - 1:2000, IHC: 1:200 - 1:1000, ICC: 1:200 - 1:1000, FCM: 1:200 - 1:400

Restrictions

For Research Use only
Format

Liquid

Buffer

Purified antibody in PBS with 0.05 % sodium azide

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

4 °C/-20 °C

Storage Comment

4°C, -20°C for long term storage
Target

ANAPC10 (Anaphase Promoting Complex Subunit 10 (ANAPC10))

Alternative Name

ANAPC10 (ANAPC10 Products)

Background

Description: ANAPC10 is a core subunit of the anaphase-promoting complex (APC), or cyclosome, a ubiquitin protein ligase that is essential for progression through the cell cycle. APC initiates sister chromatid separation by ubiquitinating the anaphase inhibitor securin (PTTG1, MIM 604147) and triggers exit from mitosis by ubiquitinating cyclin B (CCNB1, MIM 123836), the activating subunit of cyclin-dependent kinase-1 (CDK1, MIM 116940) (summary by Wendt et al., 2001 [PubMed 11524682]).,
Aliases: DOC1, APC10

Molecular Weight

21.3 kDa

Gene ID

10393

HGNC

3417

Pathways

M Phase