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HEXIM1 antibody (C-Term)

Name: HEXIM1 antibody (C-Term)
Brand: antibodies-online
SKU: ABIN334418
Price: 469.28 USD
Availability: InStock

Verified HEXIM1 Reactivity: Human, Mouse WB, ELISA, IF Host: Goat Polyclonal unconjugated

ABIN334418 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.

ABIN334418 Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.

4 images

(2 references)

Catalog No. ABIN334418

Datasheet as PDF

Target See all HEXIM1 Antibodies

HEXIM1 (Hexamethylene Bis-Acetamide Inducible 1 (HEXIM1))
Binding Specificity
13

8

6

6

6

6

3

2

2

2

2

2

2

1

1

1

1

1

1

1
C-Term
Reactivity
67

54

30

5

4

3

3

2

2

1

1

1

1

1
Human, Mouse
Host
65

7

1
Goat
Compatible Secondaries
Clonality
68

5
Polyclonal
Conjugate
41

5

5

4

4

3

1

1

1

1

1

1

1

1

1

1

1
This HEXIM1 antibody is un-conjugated
Application
69

34

33

13

12

11

6

3

2

2

2

1
Western Blotting (WB), ELISA, Immunofluorescence (IF)

Purpose

HEXIM1

Sequence

HRQQERAPLS KFGD

Cross-Reactivity

Human, Mouse

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade

Verified

Immunogen

Peptide with sequence C-HRQQERAPLSKFGD, from the C Terminus of the protein sequence according to NP_006451.1.

Isotype

IgG
anti-Hexamethylene Bis-Acetamide Inducible 1 (HEXIM1) antibody
HEXIM1 Reactivity: Human, Rat IHC, IF Host: Rabbit Polyclonal unconjugated

anti-Hexamethylene Bis-Acetamide Inducible 1 (HEXIM1) (AA 1-359) antibody
HEXIM1 Reactivity: Human WB, ELISA, IF Host: Mouse Monoclonal 1H5 unconjugated

anti-Hexamethylene Bis-Acetamide Inducible 1 (HEXIM1) antibody
HEXIM1 Reactivity: Human, Mouse, Rat WB, ELISA, IF, ICC Host: Rabbit Polyclonal unconjugated

anti-Hexamethylene Bis-Acetamide Inducible 1 (HEXIM1) antibody
HEXIM1 Reactivity: Human, Mouse, Rat WB, IF Host: Rabbit Polyclonal unconjugated

anti-Hexamethylene Bis-Acetamide Inducible 1 (HEXIM1) (AA 18-46), (N-Term) antibody
HEXIM1 Reactivity: Human, Mouse WB, IHC (p) Host: Rabbit Polyclonal RB33760 unconjugated

anti-Hexamethylene Bis-Acetamide Inducible 1 (HEXIM1) (AA 1-359) antibody
HEXIM1 Reactivity: Human WB, ELISA, IF, IHC (p) Host: Mouse Monoclonal 4G4 unconjugated

anti-Hexamethylene Bis-Acetamide Inducible 1 (HEXIM1) (AA 210-359) antibody
HEXIM1 Reactivity: Human ELISA, IHC Host: Rabbit Polyclonal unconjugated

anti-Hexamethylene Bis-Acetamide Inducible 1 (HEXIM1) (AA 194-222) antibody
HEXIM1 Reactivity: Mouse WB, IHC (p) Host: Rabbit Polyclonal RB29909 unconjugated

anti-Hexamethylene Bis-Acetamide Inducible 1 (HEXIM1) antibody
HEXIM1 Reactivity: Human, Mouse, Rat ELISA, IHC Host: Rabbit Polyclonal unconjugated

anti-Hexamethylene Bis-Acetamide Inducible 1 (HEXIM1) (AA 194-222) antibody
HEXIM1 Reactivity: Human, Mouse WB, IHC, IHC (p) Host: Rabbit Polyclonal unconjugated

Application Notes

Western Blot: Approx 40 kDa band observed in Human and Mouse Heart lysates (calculated MW of 40.6 kDa according to Human NP_006451.1 and 40.2 kDa according to Mouse NP_620092.1. Recommended concentration: 0.1-0.3 μg/mL. Primary incubation 1 hour at room tem
Peptide ELISA: antibody detection limit dilution 1:128000.

Comment

Immunofluorescence: Strong expression of the protein seen in the nucleus of A431 and HeLa cells. Recommended concentration: 10μg/ml.

Restrictions

For Research Use only
Format

Liquid

Concentration

0.5 mg/mL

Buffer

Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice

Minimize freezing and thawing.

Storage

-20 °C

Storage Comment

Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
Bugai, Quaresma, Friedel, Lenasi, Düster, Sibley, Fujinaga, Kukanja, Hennig, Blasius, Geyer, Ule, Dölken, Barborič: "P-TEFb Activation by RBM7 Shapes a Pro-survival Transcriptional Response to Genotoxic Stress." in: Molecular cell, Vol. 74, Issue 2, pp. 254-267.e10, (2019) (PubMed).

Faust, Li, Bacon, Jang, Weiss, Jayaraman, Newton, Krogan, DOrso, Frankel: "The HIV-1 Tat protein recruits a ubiquitin ligase to reorganize the 7SK snRNP for transcriptional activation." in: eLife, Vol. 7, (2019) (PubMed).
Target

HEXIM1 (Hexamethylene Bis-Acetamide Inducible 1 (HEXIM1))

Alternative Name

HEXIM1 (HEXIM1 Products)

Synonyms

HEXIM1 antibody, fd44h02 antibody, hexim antibody, wu:fd44h02 antibody, zgc:162976 antibody, CLP1 antibody, EDG1 antibody, HIS1 antibody, MAQ1 antibody, 7330426E13Rik antibody, CLP-1 antibody, Clp1 antibody, Clp-1 antibody, hexamethylene bisacetamide inducible 1 antibody, hexamethylene bis-acetamide inducible 1 antibody, hexamethylene bis-acetamide inducible 1 L homeolog antibody, HEXIM1 antibody, hexim1 antibody, Hexim1 antibody, hexim1.L antibody

Background

HEXIM1, hexamethylene bis-acetamide inducible 1, HGNC:24953, CLP1, HIS1, MAQ1 , HMBA-inducible, hexamethylene bisacetamide-inducible protein, hexamethylene-bis-acetamide-inducible transcript 1, hexamthylene bis-acetamide inducible 1, menage a quatre 1, ED

Gene ID

10614, 192231

NCBI Accession

NP_006451