BAFF Protein (Trimer) (His-DYKDDDDK Tag)
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- Target See all BAFF (TNFSF13B) Proteins
- BAFF (TNFSF13B) (Tumor Necrosis Factor (Ligand) Superfamily, Member 13b (TNFSF13B))
- Protein Type
- Recombinant
- Protein Characteristics
- Trimer
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This BAFF protein is labelled with His-DYKDDDDK Tag.
- Purpose
- Human BAFF/TNFSF13B/CD257 Trimer Protein
- Sequence
- Thr141-Leu285
- Characteristics
- Recombinant Human BAFF/TNFSF13B/CD257 Trimer Protein is expressed from HEK293 with His tag and Flag tag at the N-Terminus.It contains Thr141-Leu285.
- Purity
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human BAFF Trimer, His Tag at 0.5μg/ml (100μl/Well). Dose response curve for Human BAFFR, hFc Tag with the EC50 of 0.73μg/ml determined by ELISA. The affinity constant of 2.68 nM as determined in SPR assay (Biacore T200). See testing image for detail.
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Tumor Necrosis Factor (Ligand) Superfamily, Member 13b (TNFSF13B) (AA 134-285) protein (Fc Tag)
TNFSF13B Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Tris-Bis PAGE,> 90 % as determined by HPLC
Tumor Necrosis Factor (Ligand) Superfamily, Member 13b (TNFSF13B) (Trimer) protein (hFc-DYKDDDDK Tag)TNFSF13B Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 94 % as determined by HPLC
Tumor Necrosis Factor (Ligand) Superfamily, Member 13b (TNFSF13B) (Trimer) protein (His-Avi Tag,Biotin)TNFSF13B Origin: Human Host: HEK-293 Cells Recombinant > 95% as determined by Tris-Bis PAGE,> 94% as determined by HPLC
Tumor Necrosis Factor (Ligand) Superfamily, Member 13b (TNFSF13B) (Trimer) protein (His-Avi Tag,DYKDDDDK Tag,Biotin)TNFSF13B Origin: Cynomolgus Host: HEK-293 Cells Recombinant > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
Tumor Necrosis Factor (Ligand) Superfamily, Member 13b (TNFSF13B) (Trimer) protein (His-Avi Tag)TNFSF13B Origin: Cynomolgus Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
Tumor Necrosis Factor (Ligand) Superfamily, Member 13b (TNFSF13B) (AA 4-285) (Active) protein (His tag)TNFSF13B Origin: Human Host: Tobacco (Nicotiana benthamiana) Recombinant > 97 % by SDS polyacrylamide gel electrophoresis and the gel was stained with Coomassie blue. WB, CC Active
Tumor Necrosis Factor (Ligand) Superfamily, Member 13b (TNFSF13B) (AA 136-285) protein (His-DYKDDDDK-Strep II Tag)TNFSF13B Origin: Human Host: HEK-293T Cells Recombinant SDS, WB, ELISA, PI
Tumor Necrosis Factor (Ligand) Superfamily, Member 13b (TNFSF13B) (AA 127-309) (Active) proteinTNFSF13B Origin: Mouse Host: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active
Tumor Necrosis Factor (Ligand) Superfamily, Member 13b (TNFSF13B) (AA 140-285) (Active) protein (Fc Tag)TNFSF13B Origin: Human Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
Tumor Necrosis Factor (Ligand) Superfamily, Member 13b (TNFSF13B) (AA 140-285) (Active) protein (His tag,DYKDDDDK Tag)TNFSF13B Origin: Human Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- BAFF (TNFSF13B) (Tumor Necrosis Factor (Ligand) Superfamily, Member 13b (TNFSF13B))
- Alternative Name
- BAFF (TNFSF13B Products)
- Synonyms
- BAFF Protein, BLYS Protein, CD257 Protein, DTL Protein, TALL-1 Protein, TALL1 Protein, THANK Protein, TNFSF20 Protein, ZTNF4 Protein, BAFF-R Protein, BAFFR Protein, BROMIX Protein, CD268 Protein, CVID4 Protein, prolixin Protein, TNFSF13B Protein, RGD1561519 Protein, RGD1560810 Protein, BLyS Protein, D8Ertd387e Protein, zTNF4 Protein, 2010006P15Rik Protein, Baffr Protein, Bcmd Protein, Bcmd-1 Protein, Bcmd1 Protein, Lvis22 Protein, zgc:172115 Protein, TNF superfamily member 13b Protein, TNF receptor superfamily member 13C Protein, tumor necrosis factor (ligand) superfamily, member 13b Protein, tumor necrosis factor receptor superfamily, member 13c Protein, tumor necrosis factor superfamily member 13b Protein, TNFSF13B Protein, TNFRSF13C Protein, Tnfsf13b Protein, Tnfrsf13c Protein, tnfsf13b Protein
- Background
- B-cell activating factor (BAFF) also known as tumor necrosis factor ligand superfamily member 13B is a protein that in humans is encoded by the TNFSF13B gene.BAFF is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for receptors TNFRSF13B/TACI, TNFRSF17/BCMA, and TNFRSF13C/BAFF-R.
- Molecular Weight
- 52.4 kDa. Due to glycosylation, the protein migrates to 53-60 kDa based on Tris-Bis PAGE result.
- Pathways
- NF-kappaB Signaling, Production of Molecular Mediator of Immune Response
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