NAPA antibody (Center)
-
- Target See all NAPA Antibodies
- NAPA (N-Ethylmaleimide-Sensitive Factor Attachment Protein, alpha (NAPA))
-
Binding Specificity
- Center
-
Reactivity
- Human, Mouse, Rat
-
Host
- Rabbit
-
Clonality
- Polyclonal
-
Conjugate
- This NAPA antibody is un-conjugated
-
Application
- Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Cross-Reactivity
- Rhesus Monkey, Chimpanzee
- Cross-Reactivity (Details)
- Rhesus Monkey (100 %), Chimpanzee (100 %)
- Characteristics
-
Rabbit Polyclonal antibody to alpha SNAP (N-ethylmaleimide-sensitive factor attachment protein, alpha)
alpha SNAP antibody [N2C3] - Purification
- Purified by antigen-affinity chromatography.
- Immunogen
- Recombinant protein encompassing a sequence within the center region of human alpha SNAP. The exact sequence is proprietary.
- Isotype
- IgG
-
anti-N-Ethylmaleimide-Sensitive Factor Attachment Protein, alpha (NAPA) (AA 1-295) antibody
NAPA Reactivity: Human ELISA, WB, IHC, IF Host: Rabbit Polyclonal unconjugated
anti-N-Ethylmaleimide-Sensitive Factor Attachment Protein, alpha (NAPA) antibodyNAPA Reactivity: Human, Mouse, Rat ELISA, WB, IHC Host: Rabbit Polyclonal unconjugated
anti-N-Ethylmaleimide-Sensitive Factor Attachment Protein, alpha (NAPA) (AA 78-295) antibodyNAPA Reactivity: Human, Mouse, Rat, Monkey WB, IHC, ICC, IHC (p) Host: Rabbit Polyclonal unconjugated
anti-N-Ethylmaleimide-Sensitive Factor Attachment Protein, alpha (NAPA) antibodyNAPA Reactivity: Human WB Host: Rabbit Monoclonal unconjugated
anti-N-Ethylmaleimide-Sensitive Factor Attachment Protein, alpha (NAPA) (AA 1-295) antibodyNAPA Reactivity: Human WB, IHC, ICC Host: Rabbit Polyclonal unconjugated
anti-N-Ethylmaleimide-Sensitive Factor Attachment Protein, alpha (NAPA) (AA 1-295) antibody (APC)NAPA Reactivity: Human IHC, ICC, IF, FACS Host: Rabbit Polyclonal APC
-
- Application Notes
- Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Immunoprecipitation Assay-dependent dilution Western blot 1:1000-1:10000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* ImmunoprecipitationAssay-dependent dilution Western blot1:1000-1:10000*
- Comment
-
Positive Control: 293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji , mouse brain
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.
- Preservative
- Thimerosal (Merthiolate)
- Precaution of Use
- This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C
- Storage Comment
- Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
-
- Target
- NAPA (N-Ethylmaleimide-Sensitive Factor Attachment Protein, alpha (NAPA))
- Alternative Name
- alpha SNAP (NAPA Products)
- Synonyms
- SNAPA antibody, wu:fa26c04 antibody, zgc:63906 antibody, napb antibody, 1500039N14Rik antibody, AW209189 antibody, RA81 antibody, SNARE antibody, a-SNAP antibody, hyh antibody, alpha-SNAP antibody, Alpha-SNAP antibody, NSF attachment protein alpha antibody, N-ethylmaleimide-sensitive factor attachment protein, alpha b antibody, NSF attachment protein alpha L homeolog antibody, N-ethylmaleimide-sensitive factor attachment protein, alpha antibody, N-ethylmaleimide sensitive fusion protein attachment protein alpha antibody, soluble NSF attachment protein antibody, NAPA antibody, napab antibody, napa.L antibody, napa antibody, EHI_000300 antibody, Napa antibody, LOC102577508 antibody
- Background
-
The 'SNARE hypothesis' is a model explaining the process of docking and fusion of vesicles to their target membranes. According to this model, membrane proteins from the vesicle (v-SNAREs) and proteins from the target membrane (t-SNAREs) govern the specificity of vesicle targeting and docking through mutual recognition. Once the 2 classes of SNAREs bind to each other, they form a complex that recruits the general elements of the fusion apparatus, namely NSF (N-ethylmaleimide-sensitive factor) and SNAPs (soluble NSF-attachment proteins), to the site of membrane fusion, thereby forming the 20S fusion complex. Alpha- and gamma-SNAP are found in a wide range of tissues and act synergistically in intra-Golgi transport. The sequence of the predicted 295-amino acid human protein encoded by NAPA shares 37 % , 60 %, and 67 % identity with the sequences of yeast, Drosophila, and squid alpha-SNAP, respectively. Platelets contain some of the same proteins, including NSF, p115/TAP, alpha-SNAP, gamma-SNAP, and the t-SNAREs syntaxin-2 and syntaxin-4, that are used in many vesicular transport processes in other cell types. Platelet exocytosis uses a molecular mechanism similar to that used by other secretory cells, such as neurons, although the proteins used by the platelet and their modes of regulation may be quite different.
Cellular Localization: Membrane, Peripheral membrane protein - Molecular Weight
- 33 kDa
- Gene ID
- 8775
- Pathways
- Synaptic Vesicle Exocytosis, Asymmetric Protein Localization
-
