ATP6V1H antibody (Center)
-
- Target See all ATP6V1H Antibodies
- ATP6V1H (ATPase, H+ Transporting, Lysosomal 50/57kDa, V1 Subunit H (ATP6V1H))
-
Binding Specificity
- Center
-
Reactivity
- Human, Mouse
-
Host
- Rabbit
-
Clonality
- Polyclonal
-
Conjugate
- This ATP6V1H antibody is un-conjugated
-
Application
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Immunocytochemistry (ICC)
- Cross-Reactivity
- Chicken, Pig (Porcine), Xenopus laevis, Zebrafish (Danio rerio), Cow (Bovine), Xenopus tropicalis
- Cross-Reactivity (Details)
- Chicken (96 %), Pig (99 %), Xenopus laevis (94 %), Zebrafish (91 %), Bovine (98 %), Xenopus tropicalis (94 %)
- Characteristics
-
Rabbit Polyclonal antibody to ATP6V1H (ATPase, H+ transporting, lysosomal 50/57 kDa, V1 subunit H)
ATP6V1H antibody [N3C3] - Purification
- Purified by antigen-affinity chromatography.
- Immunogen
- Recombinant protein encompassing a sequence within the center region of human ATP6V1H. The exact sequence is proprietary.
- Isotype
- IgG
-
anti-ATPase, H+ Transporting, Lysosomal 50/57kDa, V1 Subunit H (ATP6V1H) (AA 1-483) antibody
ATP6V1H Reactivity: Human WB, IP Host: Rabbit Polyclonal unconjugated
anti-ATPase, H+ Transporting, Lysosomal 50/57kDa, V1 Subunit H (ATP6V1H) (Internal Region) antibodyATP6V1H Reactivity: Human WB, ELISA, IHC Host: Rabbit Polyclonal unconjugated
anti-ATPase, H+ Transporting, Lysosomal 50/57kDa, V1 Subunit H (ATP6V1H) antibodyATP6V1H Reactivity: Mouse, Rat WB Host: Rabbit Polyclonal unconjugated
anti-ATPase, H+ Transporting, Lysosomal 50/57kDa, V1 Subunit H (ATP6V1H) (Internal Region) antibodyATP6V1H Reactivity: Human, Mouse WB, ELISA, IHC Host: Rabbit Polyclonal unconjugated
anti-ATPase, H+ Transporting, Lysosomal 50/57kDa, V1 Subunit H (ATP6V1H) (AA 400-426), (C-Term) antibodyATP6V1H Reactivity: Human, Mouse WB Host: Rabbit Polyclonal RB25984 unconjugated
anti-ATPase, H+ Transporting, Lysosomal 50/57kDa, V1 Subunit H (ATP6V1H) (AA 1-483) antibodyATP6V1H Reactivity: Human WB Host: Mouse Polyclonal unconjugated
-
- Application Notes
- Suggested dilution Reference ICC/IF Assay-dependent dilution IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IFAssay-dependent dilution IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Western blot1:500-1:3000*
- Comment
-
Positive Control: 293T , A431 , H1299 , HeLa , Raji , mouse brain
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Concentration
- 0.56 mg/mL
- Buffer
- 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.
- Preservative
- Thimerosal (Merthiolate)
- Precaution of Use
- This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C
- Storage Comment
- Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
-
- Target
- ATP6V1H (ATPase, H+ Transporting, Lysosomal 50/57kDa, V1 Subunit H (ATP6V1H))
- Alternative Name
- ATP6V1H (ATP6V1H Products)
- Synonyms
- MSTP042 antibody, NBP1 antibody, SFD antibody, SFDalpha antibody, SFDbeta antibody, VMA13 antibody, V-ATPASE antibody, 0710001F19Rik antibody, AU022349 antibody, CGI-11 antibody, chunp6890 antibody, ATPase H+ transporting V1 subunit H antibody, ATPase, H+ transporting, lysosomal V1 subunit H antibody, ATP6V1H antibody, Atp6v1h antibody, atp6v1h antibody
- Background
-
This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c", and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This gene encodes the regulatory H subunit of the V1 domain which is required for catalysis of ATP but not the assembly of V-ATPase. Three alternatively spliced transcript variants encode two isoforms of the H subunit.
- Molecular Weight
- 56 kDa
- Gene ID
- 51606
- Pathways
- Transition Metal Ion Homeostasis, Proton Transport
-
