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EHD1 antibody (N-Term)

Verified EHD1 Reactivity: Human, Mouse WB, ELISA, IHC, IF, FACS Host: Goat Polyclonal unconjugated
Catalog No. ABIN7013871
  • Target See all EHD1 Antibodies
    EHD1 (EH-Domain Containing 1 (EHD1))
    Binding Specificity
    • 9
    • 8
    • 5
    • 5
    • 2
    • 1
    • 1
    N-Term
    Reactivity
    • 35
    • 10
    • 10
    • 3
    • 3
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    Human, Mouse
    Host
    • 35
    Goat
    Clonality
    • 34
    • 1
    Polyclonal
    Conjugate
    • 13
    • 5
    • 4
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This EHD1 antibody is un-conjugated
    Application
    • 25
    • 22
    • 4
    • 2
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)
    Purpose
    EHD1
    Sequence
    FSWVSKDARR KKEPC
    Cross-Reactivity
    Cow, Human, Mouse, Rat
    Purification
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    Grade
    Verified
  • Application Notes

    Immunohistochemistry: Paraffin embedded Human Thymus and Brain (Cortex). Recommended concentration: 3.75 μg/mL.

    Western Blot: Approx 60 kDa band observed in lysates of cell line NIH3T3 (calculated MW of 60.6 kDa according to Mouse NP_0342491) Preliminary testing also showed a band at approx 60 kDa in Human Lymph node and Rat Kidney lysate and in HeLa cell lysate. Rec

    Peptide ELISA: antibody detection limit dilution 1:32000.

    Comment

    Immunofluorescence: Strong expression of the protein seen in the membranes and cytoplasm of HeLa and U2OS cells. Recommended concentration: 10μg/ml.

    Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended c

    Restrictions
    For Research Use only
  • Buffer
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Minimize freezing and thawing.
    Storage
    -20 °C
    Storage Comment
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Target
    EHD1 (EH-Domain Containing 1 (EHD1))
    Alternative Name
    EHD1 (EHD1 Products)
    Synonyms
    AA409636 antibody, Past1 antibody, RME-1 antibody, RGD1306960 antibody, cb862 antibody, ehd1 antibody, ehd3 antibody, ehd3l antibody, wu:fb95h05 antibody, zgc:92331 antibody, MGC130676 antibody, Testilin antibody, H-PAST antibody, HPAST1 antibody, PAST antibody, PAST1 antibody, zgc:77049 antibody, EH-domain containing 1 antibody, EH-domain containing 1b antibody, EH domain containing 1 L homeolog antibody, EH domain-containing protein 1 antibody, EH domain containing 1 antibody, EH-domain containing 1a antibody, Ehd1 antibody, ehd1b antibody, ehd1.L antibody, EHD1 antibody, LOC585665 antibody, LOC100091823 antibody, ehd1a antibody
    Background
    EHD1, PAST, PAST1, H-PAST, HPAST1, EH-domain containing 1, testilin, FLJ42622, FLJ44618, OTTHUMP00000069747
    Gene ID
    10938
    Pathways
    Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development
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