LILRB4 Protein (AA 22-256) (Fc Tag)
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- Target See all LILRB4 products
- LILRB4 (Leukocyte Immunoglobulin-Like Receptor, Subfamily B (With TM and ITIM Domains), Member 4 (LILRB4))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 22-256
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This LILRB4 protein is labelled with Fc Tag.
- Purpose
- Human LILRB4/CD85k/ILT3 Protein
- Sequence
- Gln22-Arg256
- Characteristics
- Recombinant Human LILRB4/CD85k/ILT3 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Gln22-Arg256.
- Purity
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human LILRB4, hFc Tag at 0.2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Anti-LILRB4 Antibody, hFc Tag with the EC50 of 16.7ng/ml determined by ELISA. The affinity constant of 115.9 nM as determined in SPR assay (Biacore T200). See testing image for detail.
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Leukocyte Immunoglobulin-Like Receptor, Subfamily B (With TM and ITIM Domains), Member 4 (LILRB4) (AA 22-257) protein (His-Avi Tag)
LILRB4 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Leukocyte Immunoglobulin-Like Receptor, Subfamily B (With TM and ITIM Domains), Member 4 (LILRB4) (AA 22-259) protein (His tag)LILRB4 Origin: Cynomolgus Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Leukocyte Immunoglobulin-Like Receptor, Subfamily B (With TM and ITIM Domains), Member 4 (LILRB4) (AA 24-238) protein (His tag)LILRB4 Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Leukocyte Immunoglobulin-Like Receptor, Subfamily B (With TM and ITIM Domains), Member 4 (LILRB4) (AA 22-257) protein (His-Avi Tag,Biotin)LILRB4 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Leukocyte Immunoglobulin-Like Receptor, Subfamily B (With TM and ITIM Domains), Member 4 (LILRB4) (AA 1-448) protein (GST tag)LILRB4 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Leukocyte Immunoglobulin-Like Receptor, Subfamily B (With TM and ITIM Domains), Member 4 (LILRB4) (AA 22-118) protein (His-Avi Tag)LILRB4 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Leukocyte Immunoglobulin-Like Receptor, Subfamily B (With TM and ITIM Domains), Member 4 (LILRB4) (AA 22-118) protein (His-Avi Tag,Biotin)LILRB4 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Leukocyte Immunoglobulin-Like Receptor, Subfamily B (With TM and ITIM Domains), Member 4 (LILRB4) (AA 119-259) protein (His-Avi Tag,Biotin)LILRB4 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
Leukocyte Immunoglobulin-Like Receptor, Subfamily B (With TM and ITIM Domains), Member 4 (LILRB4) (AA 24-238) protein (His tag)Crystallography grade LILRB4 Origin: Mouse Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Leukocyte Immunoglobulin-Like Receptor, Subfamily B (With TM and ITIM Domains), Member 4 (LILRB4) (AA 281-448) protein (His tag)Crystallography grade LILRB4 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- LILRB4 (Leukocyte Immunoglobulin-Like Receptor, Subfamily B (With TM and ITIM Domains), Member 4 (LILRB4))
- Alternative Name
- LILRB4 (LILRB4 Products)
- Synonyms
- CD85K Protein, ILT-3 Protein, ILT3 Protein, LIR-5 Protein, LIR5 Protein, Gp49b Protein, HM18 Protein, gp49 Protein, leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains), member 4 Protein, leukocyte immunoglobulin like receptor B4 Protein, leukocyte immunoglobulin-like receptor, subfamily B, member 4A Protein, LILRB4 Protein, Lilrb4a Protein
- Background
- LILRB4,also known as CD85k and LIR-5, ILT3, is an approximately 60 kDa transmembrane glycoprotein that negatively regulates immune cell activation. Mature human ILT3 consists of a 238 amino acid (aa) extracellular domain with two Ig-like domains, a 21 aa transmembrane segment, and a 168 aa cytoplasmic domain with 3 immunoreceptor tyrosine-based inhibitory motifs (ITIM).LILRB4 is receptor for class I MHC antigens. Recognizes a broad spectrum of HLA-A, HLA-B, HLA-C and HLA-G alleles.
- Molecular Weight
- 52.6 kDa. Due to glycosylation, the protein migrates to 60-68 kDa based on Tris-Bis PAGE result.
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