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Choline Acetyltransferase antibody (AA 517-732)

CHAT Reactivity: Human WB, ELISA, ICC, IHC (p), IHC (fro) Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN7440704
  • Target See all Choline Acetyltransferase (CHAT) Antibodies
    Choline Acetyltransferase (CHAT) (Choline O-Acetyltransferase (CHAT))
    Binding Specificity
    • 16
    • 15
    • 6
    • 6
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 517-732
    Reactivity
    • 70
    • 44
    • 39
    • 17
    • 5
    • 3
    • 2
    • 1
    • 1
    • 1
    Human
    Host
    • 65
    • 7
    • 5
    • 3
    • 3
    Rabbit
    Clonality
    • 67
    • 16
    Polyclonal
    Conjugate
    • 47
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This Choline Acetyltransferase antibody is un-conjugated
    Application
    • 44
    • 25
    • 24
    • 23
    • 16
    • 15
    • 13
    • 13
    • 7
    • 6
    • 5
    • 3
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
    Purpose
    Polyclonal Antibody to Choline Acetyltransferase (ChAT)
    Specificity
    The antibody is a rabbit polyclonal antibody raised against ChAT. It has been selected for its ability to recognize ChAT in immunohistochemical staining and western blotting.
    Cross-Reactivity
    Mouse, Rat
    Purification
    Antigen-specific affinity chromatography followed by Protein A affinity chromatography
    Immunogen
    Recombinant Choline Acetyltransferase (ChAT) corresdonding to Asp517~Pro732 with N-terminal His Tag
    Isotype
    IgG
  • Application Notes

    Western blotting: 0.5-2 μg/mL

    Immunohistochemistry: 5-20 μg/mL

    Immunocytochemistry: 5-20 μg/mL

    Optimal working dilutions must be determined by end user.

    Comment

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.
    Preservative
    ProClin
    Precaution of Use
    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Storage Comment
    Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
    Expiry Date
    24 months
  • Target
    Choline Acetyltransferase (CHAT) (Choline O-Acetyltransferase (CHAT))
    Alternative Name
    Choline Acetyltransferase (CHAT Products)
    Synonyms
    CHOACTASE antibody, CMS1A antibody, CMS1A2 antibody, ChoAcTase antibody, B230380D24Rik antibody, choline O-acetyltransferase antibody, choline O-acetyltransferase a antibody, choline acetyltransferase antibody, carnitine O-acetyltransferase antibody, CHAT antibody, chata antibody, Chat antibody, PGTG_00551 antibody, LOC100533322 antibody
    Background
    CMS1A, CMS1A2, CHOACTase, Choline acetylase
    Pathways
    Skeletal Muscle Fiber Development
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