POLR3A antibody (N-Term)
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- Target See all POLR3A Antibodies
- POLR3A (Polymerase (RNA) III (DNA Directed) Polypeptide A, 155kDa (POLR3A))
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Binding Specificity
- N-Term
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This POLR3A antibody is un-conjugated
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Application
- Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunoprecipitation (IP)
- Cross-Reactivity
- Mouse (Murine), Chicken, Xenopus laevis, Cow (Bovine), Xenopus tropicalis
- Cross-Reactivity (Details)
- Mouse (97 %), Chicken (97 %), Xenopus laevis (94 %), Bovine (98 %), Xenopus tropicalis (94 %)
- Characteristics
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Rabbit Polyclonal antibody to POLR3A (polymerase (RNA) III (DNA directed) polypeptide A, 155 kDa)
POLR3A antibody - Purification
- Purified by antigen-affinity chromatography.
- Immunogen
- Recombinant protein encompassing a sequence within the N-terminus region of human POLR3A. The exact sequence is proprietary.
- Isotype
- IgG
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anti-Polymerase (RNA) III (DNA Directed) Polypeptide A, 155kDa (POLR3A) (AA 392-632) antibody
POLR3A Reactivity: Human ELISA, IHC, ChIP Host: Rabbit Polyclonal unconjugated
anti-Polymerase (RNA) III (DNA Directed) Polypeptide A, 155kDa (POLR3A) (N-Term) antibodyPOLR3A Reactivity: Human ELISA, WB, IHC Host: Rabbit Polyclonal unconjugated
anti-Polymerase (RNA) III (DNA Directed) Polypeptide A, 155kDa (POLR3A) (N-Term) antibodyPOLR3A Reactivity: Human, Mouse ELISA, WB, IF, ICC Host: Rabbit Polyclonal unconjugated
anti-Polymerase (RNA) III (DNA Directed) Polypeptide A, 155kDa (POLR3A) (N-Term) antibodyPOLR3A Reactivity: Human, Mouse, Monkey ELISA, WB, IHC Host: Rabbit Polyclonal unconjugated
anti-Polymerase (RNA) III (DNA Directed) Polypeptide A, 155kDa (POLR3A) (C-Term) antibodyPOLR3A Reactivity: Human, Mouse, Rat, Monkey, Dog, Rabbit, Horse WB Host: Rabbit Polyclonal unconjugated
anti-Polymerase (RNA) III (DNA Directed) Polypeptide A, 155kDa (POLR3A) (AA 1-200) antibodyPOLR3A Reactivity: Human WB, IHC Host: Rabbit Polyclonal unconjugated
anti-Polymerase (RNA) III (DNA Directed) Polypeptide A, 155kDa (POLR3A) (N-Term) antibodyPOLR3A Reactivity: Human, Mouse, Rat, Monkey, Dog, Rabbit, Cow, Chicken, Pig, Zebrafish (Danio rerio) WB, IHC Host: Rabbit Polyclonal unconjugated
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- Application Notes
- Suggested dilution Reference ICC/IF 1:100-1:1000* Immunoprecipitation 1:100-1:500* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* Immunoprecipitation1:100-1:500* Western blot1:500-1:3000*
- Comment
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Positive Control: HCT116 , SK-N-SH , IMR32 , SK-N-AS
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1.49 mg/mL
- Buffer
- 1XPBS, 20 % Glycerol ( pH 7). 0.025 % ProClin 300 was added as a preservative.
- Preservative
- ProClin
- Precaution of Use
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C
- Storage Comment
- Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
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- Target
- POLR3A (Polymerase (RNA) III (DNA Directed) Polypeptide A, 155kDa (POLR3A))
- Alternative Name
- POLR3A (POLR3A Products)
- Synonyms
- 9330175N20Rik antibody, BC053071 antibody, RPC1 antibody, RPC155 antibody, RGD1305574 antibody, ADDH antibody, HLD7 antibody, hRPC155 antibody, polymerase (RNA) III (DNA directed) polypeptide A antibody, RNA polymerase III subunit A antibody, Polr3a antibody, POLR3A antibody
- Background
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DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Forms the polymerase active center together with the second largest subunit. A single stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol III. A bridging helix emanates from RPC1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol III by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition (By similarity). Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway.
Cellular Localization: Nucleus - Molecular Weight
- 156 kDa
- Gene ID
- 11128
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