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CHEK2 antibody (pThr387)

CHEK2 Reactivity: Human, Mouse, Rat WB, ELISA, IHC, IF, ICC Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN6256554
  • Target See all CHEK2 Antibodies
    CHEK2 (Checkpoint Kinase 2 (CHEK2))
    Binding Specificity
    • 30
    • 27
    • 21
    • 17
    • 16
    • 15
    • 15
    • 15
    • 9
    • 8
    • 8
    • 8
    • 8
    • 7
    • 7
    • 6
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    pThr387
    Reactivity
    • 260
    • 95
    • 81
    • 16
    • 14
    • 7
    • 6
    • 5
    • 2
    • 2
    • 2
    • 1
    • 1
    Human, Mouse, Rat
    Host
    • 241
    • 20
    • 1
    • 1
    Rabbit
    Clonality
    • 227
    • 36
    Polyclonal
    Conjugate
    • 115
    • 14
    • 12
    • 12
    • 12
    • 12
    • 12
    • 12
    • 10
    • 9
    • 7
    • 7
    • 7
    • 7
    • 7
    • 2
    • 2
    • 2
    • 1
    • 1
    This CHEK2 antibody is un-conjugated
    Application
    • 205
    • 80
    • 79
    • 71
    • 36
    • 35
    • 33
    • 28
    • 28
    • 19
    • 17
    • 5
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)
    Specificity
    Phospho-Chk2 (Thr387) Antibody detects endogenous levels of Chk2 only when phosphorylated at Threonine 387.
    Predicted Reactivity
    Pig,Zebrafish,Bovine,Horse,Sheep,Rabbit,Dog,Chicken,Xenopus
    Purification
    The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
    Immunogen
    A synthesized peptide derived from human Chk2 around the phosphorylation site of Thr387.
    Isotype
    IgG
  • Application Notes
    WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 mg/mL
    Buffer
    Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    -20 °C
    Storage Comment
    Store at -20 °C. Stable for 12 months from date of receipt.
    Expiry Date
    12 months
  • Mousa, Soliman, Alhumaydhi, Almatroudi, Al Rugaie, Allemailem, Alrumaihi, Khan, Rezk, Aljasir, Alwashmi, Aba Alkhayl, Albutti, Seleem: "Garlic Extract Alleviates Trastuzumab-Induced Hepatotoxicity in Rats Through Its Antioxidant, Anti-Inflammatory, and Antihyperlipidemic Effects." in: Journal of inflammation research, Vol. 14, pp. 6305-6316, (2021) (PubMed).

  • Target
    CHEK2 (Checkpoint Kinase 2 (CHEK2))
    Alternative Name
    CHEK2 (CHEK2 Products)
    Synonyms
    CDS1 antibody, CHK2 antibody, HuCds1 antibody, LFS2 antibody, PP1425 antibody, RAD53 antibody, hCds1 antibody, fa66f08 antibody, wu:fa66f08 antibody, zgc:55865 antibody, Cds1 antibody, HUCDS1 antibody, Rad53 antibody, Chk2 antibody, cds1 antibody, chek2 antibody, chk2 antibody, hucds1 antibody, lfs2 antibody, pp1425 antibody, rad53 antibody, checkpoint kinase 2 antibody, serine/threonine-protein kinase chk2 antibody, checkpoint kinase 2 L homeolog antibody, CHEK2 antibody, chek2 antibody, Chek2 antibody, MCYG_07308 antibody, chek2.L antibody
    Background

    Description: Serine/threonine-protein kinase which is required for checkpoint-mediated cell cycle arrest, activation of DNA repair and apoptosis in response to the presence of DNA double-strand breaks. May also negatively regulate cell cycle progression during unperturbed cell cycles. Following activation, phosphorylates numerous effectors preferentially at the consensus sequence [L-X-R-X-X-S/T]. Regulates cell cycle checkpoint arrest through phosphorylation of CDC25A, CDC25B and CDC25C, inhibiting their activity. Inhibition of CDC25 phosphatase activity leads to increased inhibitory tyrosine phosphorylation of CDK-cyclin complexes and blocks cell cycle progression. May also phosphorylate NEK6 which is involved in G2/M cell cycle arrest. Regulates DNA repair through phosphorylation of BRCA2, enhancing the association of RAD51 with chromatin which promotes DNA repair by homologous recombination. Also stimulates the transcription of genes involved in DNA repair (including BRCA2) through the phosphorylation and activation of the transcription factor FOXM1. Regulates apoptosis through the phosphorylation of p53/TP53, MDM4 and PML. Phosphorylation of p53/TP53 at 'Ser-20' by CHEK2 may alleviate inhibition by MDM2, leading to accumulation of active p53/TP53. Phosphorylation of MDM4 may also reduce degradation of p53/TP53. Also controls the transcription of pro-apoptotic genes through phosphorylation of the transcription factor E2F1. Tumor suppressor, it may also have a DNA damage-independent function in mitotic spindle assembly by phosphorylating BRCA1. Its absence may be a cause of the chromosomal instability observed in some cancer cells. Promotes the CCAR2-SIRT1 association and is required for CCAR2-mediated SIRT1 inhibition (PubMed:25361978).

    Gene: CHEK2

    Molecular Weight
    62kDa
    Gene ID
    11200
    UniProt
    O96017
    Pathways
    p53 Signaling, Apoptosis, Cell Division Cycle
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